Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L.
Objective: The contents of main active ingredients, antioxidant and cholate binding ability of different ethanol extracts from Psoralea corylifolia L. were explored based on the mode of correlation of “components-efficacy”. Methods: Psoralea corylifolia L. was extracted with anhydrous ethanol, 75% e...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-07-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010218 |
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| author | Xinyang JIANG Jinyue LIANG Jitong SHI Lanling LIU Shanshan ZHANG Pan ZHAO Peizheng YAN Dongsheng ZHAO |
| author_facet | Xinyang JIANG Jinyue LIANG Jitong SHI Lanling LIU Shanshan ZHANG Pan ZHAO Peizheng YAN Dongsheng ZHAO |
| author_sort | Xinyang JIANG |
| collection | DOAJ |
| description | Objective: The contents of main active ingredients, antioxidant and cholate binding ability of different ethanol extracts from Psoralea corylifolia L. were explored based on the mode of correlation of “components-efficacy”. Methods: Psoralea corylifolia L. was extracted with anhydrous ethanol, 75% ethanol, 50% ethanol and water. The contents of total flavonoids and total polyphenols in Psoralea corylifolia L. were determined. The antioxidant and lipid-lowering activities in vitro of Psoralea corylifolia L. were evaluated by three antioxidant systems and the binding ability of cholate salts, respectively, and the correlation analysis was conducted. Results: The contents of total flavonoids and total polyphenols in anhydrous ethanol extract were the highest, (50.317±0.018) mg/g and (3.860±0.045) mg/g, respectively. The 50% ethanol extract showed the best ability to scavenge ABTS＋· and DPPH·, the IC50 values were 0.76 and 1.63 mg/mL, respectively. The anhydrous ethanol extract showed the strongest reducing ability, the A0.5 value was 2.63 mg/mL. The water extract showed the strongest binding capacity to sodium cholate, the IC50 value was 0.67 mg/mL. The 50% ethanol extract showed the strongest binding capacity to sodium taurocholate and sodium glycinate, the IC50 values were 1.26 and 0.41 mg/mL, respectively. The correlation results showed that the content of total flavonoids of different ethanol extracts from Psoralea corylifolia L. had a significant positive correlation with DPPH· clearance rate and sodium taurocholate binding rate (P<0.05). And the content of total flavonoids and total polyphenols had a very significant positive correlation with reduction ability (P<0.01). While the content of total polyphenols had a significant negative correlation with sodium cholate binding rate (P<0.05). Conclusion: The 50% ethanol extract of Psoralea corylifolia L. has high content of active ingredients and the strongest antioxidant and cholate binding ability. It can be used as a potential important source of natural antioxidant and lipid-lowering active substances. |
| first_indexed | 2024-04-13T09:11:25Z |
| format | Article |
| id | doaj.art-80e48619c7cc4300abc62b79901aea9c |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:11:25Z |
| publishDate | 2022-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-80e48619c7cc4300abc62b79901aea9c2022-12-22T02:52:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-01431338138810.13386/j.issn1002-0306.20220102182022010218-13Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L.Xinyang JIANG0Jinyue LIANG1Jitong SHI2Lanling LIU3Shanshan ZHANG4Pan ZHAO5Peizheng YAN6Dongsheng ZHAO7College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaExperimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaObjective: The contents of main active ingredients, antioxidant and cholate binding ability of different ethanol extracts from Psoralea corylifolia L. were explored based on the mode of correlation of “components-efficacy”. Methods: Psoralea corylifolia L. was extracted with anhydrous ethanol, 75% ethanol, 50% ethanol and water. The contents of total flavonoids and total polyphenols in Psoralea corylifolia L. were determined. The antioxidant and lipid-lowering activities in vitro of Psoralea corylifolia L. were evaluated by three antioxidant systems and the binding ability of cholate salts, respectively, and the correlation analysis was conducted. Results: The contents of total flavonoids and total polyphenols in anhydrous ethanol extract were the highest, (50.317±0.018) mg/g and (3.860±0.045) mg/g, respectively. The 50% ethanol extract showed the best ability to scavenge ABTS＋· and DPPH·, the IC50 values were 0.76 and 1.63 mg/mL, respectively. The anhydrous ethanol extract showed the strongest reducing ability, the A0.5 value was 2.63 mg/mL. The water extract showed the strongest binding capacity to sodium cholate, the IC50 value was 0.67 mg/mL. The 50% ethanol extract showed the strongest binding capacity to sodium taurocholate and sodium glycinate, the IC50 values were 1.26 and 0.41 mg/mL, respectively. The correlation results showed that the content of total flavonoids of different ethanol extracts from Psoralea corylifolia L. had a significant positive correlation with DPPH· clearance rate and sodium taurocholate binding rate (P<0.05). And the content of total flavonoids and total polyphenols had a very significant positive correlation with reduction ability (P<0.01). While the content of total polyphenols had a significant negative correlation with sodium cholate binding rate (P<0.05). Conclusion: The 50% ethanol extract of Psoralea corylifolia L. has high content of active ingredients and the strongest antioxidant and cholate binding ability. It can be used as a potential important source of natural antioxidant and lipid-lowering active substances.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010218psoralea corylifolia l.ethanol extractsantioxidantcholatecorrelation analysishypolipidemic |
| spellingShingle | Xinyang JIANG Jinyue LIANG Jitong SHI Lanling LIU Shanshan ZHANG Pan ZHAO Peizheng YAN Dongsheng ZHAO Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. Shipin gongye ke-ji psoralea corylifolia l. ethanol extracts antioxidant cholate correlation analysis hypolipidemic |
| title | Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. |
| title_full | Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. |
| title_fullStr | Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. |
| title_full_unstemmed | Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. |
| title_short | Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L. |
| title_sort | correlation analysis between the active ingredients and the antioxidant or cholate binding ability of ethanol extracts from psoralea corylifolia l |
| topic | psoralea corylifolia l. ethanol extracts antioxidant cholate correlation analysis hypolipidemic |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022010218 |
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