Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation
Bacterial keratitis is a widespread disease in which bacteria have infected the cornea. This can lead to blindness and should be treated immediately after diagnosis. However, antibiotics do not reach pathogens inside the cornea. Therefore, it is investigated whether UVB irradiation might be a feasib...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-09-01
|
| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2023-1012 |
| _version_ | 1827780170554015744 |
|---|---|
| author | Fischer Simone Fehler Nicole Hessling Martin |
| author_facet | Fischer Simone Fehler Nicole Hessling Martin |
| author_sort | Fischer Simone |
| collection | DOAJ |
| description | Bacterial keratitis is a widespread disease in which bacteria have infected the cornea. This can lead to blindness and should be treated immediately after diagnosis. However, antibiotics do not reach pathogens inside the cornea. Therefore, it is investigated whether UVB irradiation might be a feasible therapy that reaches and inactivates bacteria even deep inside the cornea. Thus, the cornea transmission of porcine eyes was measured to reveal a high UVB penetration. As Escherichia coli (E. coli) is one of the relevant pathogens, E. coli suspensions were irradiated by a 311 nm UVB lamp, to explore the antimicrobial impact of the UVB radiation. Finally, as a worst-case scenario, it was investigated whether E. coli behind the cornea was also inactivated. A cornea transmission of 36.4 ± 5.9 % was observed at 311 nm and an irradiation dosage of 1.9 J/cm2 was found for a three log reduction of E. coli in suspension. The reduction of E. coli by 311 nm irradiation even behind the cornea could be confirmed. Therefore, by this approach, it should be possible to reach bacteria even deep inside the cornea by means of UVB irradiation as a fast keratitis therapy. |
| first_indexed | 2024-03-11T15:00:53Z |
| format | Article |
| id | doaj.art-80e5228cb4084056a4b0f692dd76dd74 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-03-11T15:00:53Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-80e5228cb4084056a4b0f692dd76dd742023-10-30T07:58:11ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042023-09-0191454810.1515/cdbme-2023-1012Feasibility study for a possible therapy of bacterial keratitis by UVB irradiationFischer Simone0Fehler NicoleHessling MartinInstitute of Medical Engineering and Mechatronics, Ulm University of Applied Sciences, Ulm, GermanyBacterial keratitis is a widespread disease in which bacteria have infected the cornea. This can lead to blindness and should be treated immediately after diagnosis. However, antibiotics do not reach pathogens inside the cornea. Therefore, it is investigated whether UVB irradiation might be a feasible therapy that reaches and inactivates bacteria even deep inside the cornea. Thus, the cornea transmission of porcine eyes was measured to reveal a high UVB penetration. As Escherichia coli (E. coli) is one of the relevant pathogens, E. coli suspensions were irradiated by a 311 nm UVB lamp, to explore the antimicrobial impact of the UVB radiation. Finally, as a worst-case scenario, it was investigated whether E. coli behind the cornea was also inactivated. A cornea transmission of 36.4 ± 5.9 % was observed at 311 nm and an irradiation dosage of 1.9 J/cm2 was found for a three log reduction of E. coli in suspension. The reduction of E. coli by 311 nm irradiation even behind the cornea could be confirmed. Therefore, by this approach, it should be possible to reach bacteria even deep inside the cornea by means of UVB irradiation as a fast keratitis therapy.https://doi.org/10.1515/cdbme-2023-1012bacterial keratitisporcine corneasescherichia coli311 nmuvb |
| spellingShingle | Fischer Simone Fehler Nicole Hessling Martin Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation Current Directions in Biomedical Engineering bacterial keratitis porcine corneas escherichia coli 311 nm uvb |
| title | Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation |
| title_full | Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation |
| title_fullStr | Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation |
| title_full_unstemmed | Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation |
| title_short | Feasibility study for a possible therapy of bacterial keratitis by UVB irradiation |
| title_sort | feasibility study for a possible therapy of bacterial keratitis by uvb irradiation |
| topic | bacterial keratitis porcine corneas escherichia coli 311 nm uvb |
| url | https://doi.org/10.1515/cdbme-2023-1012 |
| work_keys_str_mv | AT fischersimone feasibilitystudyforapossibletherapyofbacterialkeratitisbyuvbirradiation AT fehlernicole feasibilitystudyforapossibletherapyofbacterialkeratitisbyuvbirradiation AT hesslingmartin feasibilitystudyforapossibletherapyofbacterialkeratitisbyuvbirradiation |