Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study
Abstract Background Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell in...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-017-2442-5 |
| _version_ | 1811206529819344896 |
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| author | Jingge Zhao Takashi Matsuba Xiaoyan Zhang Susan Leano Chie Nakajima Haorile Chagan-Yasutan Elizabeth Freda Telan Yasuhiko Suzuki Toshio Hattori |
| author_facet | Jingge Zhao Takashi Matsuba Xiaoyan Zhang Susan Leano Chie Nakajima Haorile Chagan-Yasutan Elizabeth Freda Telan Yasuhiko Suzuki Toshio Hattori |
| author_sort | Jingge Zhao |
| collection | DOAJ |
| description | Abstract Background Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses. However, the immune response to Rv0679c in humans remains unknown. Therefore, we aimed to investigate the anti-Rv0679c immune response in TB patients from the endemic and non-endemic regions of the Beijing MTB genotype. Methods We analyzed the Rv0679c-specific antibody responses in 84 subjects from the endemic region of the Beijing genotype MTB in China, including 45 pulmonary TB patients (C-PTB) and 39 healthy controls (C-HC), and 81 subjects from the Philippines (the endemic region of the non-Beijing genotype), including 51 pulmonary TB patients (P-PTB) and 30 healthy controls (P-HC). Anti-tuberculous-glycolipid (TBGL) antigen was used as the control antibody. Results TBGL IgG titers were higher in both C-PTB and P-PTB than those in their corresponding HC (C-PTB median 4.2, P-PTB median 11.2; C-PTB vs. P-PTB, p > 0.05), suggesting immune response comparability in PTB from two different countries. C-PTB showed a higher response compared to C-HC for anti-K-type IgG (53.3%) than anti-N-type IgG (6.67%); this response was not observed in P-PTB (both N-type and K-type 9.80%). Conclusion Dimorphic antigen Rv0679c was found to be associated with distinct immune response patterns, indicating the role of Beijing/non-Beijing genotype of MTB in stimulating specific responses in TB patients from the endemic region of Beijing MTB. Meanwhile, reactions to Rv0679c in patients and HC from non-endemic regions of the Beijing MTB may be caused by the response to the common epitope of Rv0679c N/K-type. |
| first_indexed | 2024-04-12T03:48:39Z |
| format | Article |
| id | doaj.art-80e6212170f442629aaa63d3d653f8ec |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-12T03:48:39Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-80e6212170f442629aaa63d3d653f8ec2022-12-22T03:49:03ZengBMCBMC Infectious Diseases1471-23342017-05-0117111310.1186/s12879-017-2442-5Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control studyJingge Zhao0Takashi Matsuba1Xiaoyan Zhang2Susan Leano3Chie Nakajima4Haorile Chagan-Yasutan5Elizabeth Freda Telan6Yasuhiko Suzuki7Toshio Hattori8Laboratory of Disaster Medicine, International Research Institute of Disaster Science, Tohoku UniversityDivision of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori UniversityShanghai Public Health Clinical Center, Fudan UniveristySTD AIDS Cooperative Central Laboratory, San Lazaro HospitalDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis ControlLaboratory of Disaster Medicine, International Research Institute of Disaster Science, Tohoku UniversitySTD AIDS Cooperative Central Laboratory, San Lazaro HospitalDivision of Global Epidemiology, Hokkaido University Research Center for Zoonosis ControlGraduate School of Health Science Studies, Kibi International UniversityAbstract Background Strains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses. However, the immune response to Rv0679c in humans remains unknown. Therefore, we aimed to investigate the anti-Rv0679c immune response in TB patients from the endemic and non-endemic regions of the Beijing MTB genotype. Methods We analyzed the Rv0679c-specific antibody responses in 84 subjects from the endemic region of the Beijing genotype MTB in China, including 45 pulmonary TB patients (C-PTB) and 39 healthy controls (C-HC), and 81 subjects from the Philippines (the endemic region of the non-Beijing genotype), including 51 pulmonary TB patients (P-PTB) and 30 healthy controls (P-HC). Anti-tuberculous-glycolipid (TBGL) antigen was used as the control antibody. Results TBGL IgG titers were higher in both C-PTB and P-PTB than those in their corresponding HC (C-PTB median 4.2, P-PTB median 11.2; C-PTB vs. P-PTB, p > 0.05), suggesting immune response comparability in PTB from two different countries. C-PTB showed a higher response compared to C-HC for anti-K-type IgG (53.3%) than anti-N-type IgG (6.67%); this response was not observed in P-PTB (both N-type and K-type 9.80%). Conclusion Dimorphic antigen Rv0679c was found to be associated with distinct immune response patterns, indicating the role of Beijing/non-Beijing genotype of MTB in stimulating specific responses in TB patients from the endemic region of Beijing MTB. Meanwhile, reactions to Rv0679c in patients and HC from non-endemic regions of the Beijing MTB may be caused by the response to the common epitope of Rv0679c N/K-type.http://link.springer.com/article/10.1186/s12879-017-2442-5Mycobacterium tuberculosisDimorphic antigenRv0679cBeijing genotypePhilippinesImmunoglobulin G |
| spellingShingle | Jingge Zhao Takashi Matsuba Xiaoyan Zhang Susan Leano Chie Nakajima Haorile Chagan-Yasutan Elizabeth Freda Telan Yasuhiko Suzuki Toshio Hattori Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study BMC Infectious Diseases Mycobacterium tuberculosis Dimorphic antigen Rv0679c Beijing genotype Philippines Immunoglobulin G |
| title | Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study |
| title_full | Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study |
| title_fullStr | Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study |
| title_full_unstemmed | Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study |
| title_short | Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study |
| title_sort | comparison of antibody responses against mycobacterium tuberculosis antigen rv0679c in tuberculosis patients from the endemic and non endemic regions of the beijing genotype a case control study |
| topic | Mycobacterium tuberculosis Dimorphic antigen Rv0679c Beijing genotype Philippines Immunoglobulin G |
| url | http://link.springer.com/article/10.1186/s12879-017-2442-5 |
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