Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em>
Fungi constituting the <i>Entomophthora muscae</i> species complex (members of subphylum <i>Entomophthoromycotina</i>, phylum <i>Zoopagamycota</i>) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public...
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MDPI AG
2019-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/11/4/351 |
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| author | Max L. Nibert Humberto J. Debat Austin R. Manny Igor V. Grigoriev Henrik H. De Fine Licht |
| author_facet | Max L. Nibert Humberto J. Debat Austin R. Manny Igor V. Grigoriev Henrik H. De Fine Licht |
| author_sort | Max L. Nibert |
| collection | DOAJ |
| description | Fungi constituting the <i>Entomophthora muscae</i> species complex (members of subphylum <i>Entomophthoromycotina</i>, phylum <i>Zoopagamycota</i>) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different <i>E. muscae</i> isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum <i>Glomeromyoctina</i>, phylum <i>Mucoromycota</i>, phylum <i>Basidiomycota</i> and phylum <i>Ascomycota</i>) as well as in plants. <i>E. muscae</i> is the first fungus from phylum <i>Zoopagomycota</i>, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from <i>E. muscae</i> but also in mitochondrial core-gene coding sequences newly assembled from <i>E. muscae</i> transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses. |
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| id | doaj.art-80e7bc9b7b5744218b50440367f5a5fc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-13T18:17:04Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-80e7bc9b7b5744218b50440367f5a5fc2022-12-21T23:35:49ZengMDPI AGViruses1999-49152019-04-0111435110.3390/v11040351v11040351Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em>Max L. Nibert0Humberto J. Debat1Austin R. Manny2Igor V. Grigoriev3Henrik H. De Fine Licht4Department of Microbiology and Program in Virology, Harvard Medical School, Boston, MA 02115, USAInstituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Córdoba X5020ICA, ArgentinaDepartment of Microbiology and Program in Virology, Harvard Medical School, Boston, MA 02115, USAU.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USADepartment of Plant and Environmental Sciences, University of Copenhagen, DK-1871 Frederiksberg, DenmarkFungi constituting the <i>Entomophthora muscae</i> species complex (members of subphylum <i>Entomophthoromycotina</i>, phylum <i>Zoopagamycota</i>) commonly kill their insect hosts and manipulate host behaviors in the process. In this study, we made use of public transcriptome data to identify and characterize eight new species of mitoviruses associated with several different <i>E. muscae</i> isolates. Mitoviruses are simple RNA viruses that replicate in host mitochondria and are frequently found in more phylogenetically apical fungi (members of subphylum <i>Glomeromyoctina</i>, phylum <i>Mucoromycota</i>, phylum <i>Basidiomycota</i> and phylum <i>Ascomycota</i>) as well as in plants. <i>E. muscae</i> is the first fungus from phylum <i>Zoopagomycota</i>, and thereby the most phylogenetically basal fungus, found to harbor mitoviruses to date. Multiple UGA (Trp) codons are found not only in each of the new mitovirus sequences from <i>E. muscae</i> but also in mitochondrial core-gene coding sequences newly assembled from <i>E. muscae</i> transcriptome data, suggesting that UGA (Trp) is not a rarely used codon in the mitochondria of this fungus. The presence of mitoviruses in these basal fungi has possible implications for the evolution of these viruses.https://www.mdpi.com/1999-4915/11/4/351database mining<i>Entomophthora</i><i>Entomophthoromycotina</i>fungal virusmitochondrionmycovirusvirus discovery<i>Mitovirus</i><i>Narnaviridae</i> |
| spellingShingle | Max L. Nibert Humberto J. Debat Austin R. Manny Igor V. Grigoriev Henrik H. De Fine Licht Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> Viruses database mining <i>Entomophthora</i> <i>Entomophthoromycotina</i> fungal virus mitochondrion mycovirus virus discovery <i>Mitovirus</i> <i>Narnaviridae</i> |
| title | Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> |
| title_full | Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> |
| title_fullStr | Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> |
| title_full_unstemmed | Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> |
| title_short | Mitovirus and Mitochondrial Coding Sequences from Basal Fungus <em>Entomophthora muscae</em> |
| title_sort | mitovirus and mitochondrial coding sequences from basal fungus em entomophthora muscae em |
| topic | database mining <i>Entomophthora</i> <i>Entomophthoromycotina</i> fungal virus mitochondrion mycovirus virus discovery <i>Mitovirus</i> <i>Narnaviridae</i> |
| url | https://www.mdpi.com/1999-4915/11/4/351 |
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