Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs
Two aspects of the biosynthesis of the non-canonical terpene synthase for 2-methylisoborneol have been studied. Several 2-methylisoborneol synthases have a proline-rich N-terminal domain of unknown function. The results presented here demonstrate that this domain leads to a reduced enzyme activity,...
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2023-09-01
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| Series: | Beilstein Journal of Organic Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjoc.19.104 |
| _version_ | 1797668576641941504 |
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| author | Binbin Gu Lin-Fu Liang Jeroen S. Dickschat |
| author_facet | Binbin Gu Lin-Fu Liang Jeroen S. Dickschat |
| author_sort | Binbin Gu |
| collection | DOAJ |
| description | Two aspects of the biosynthesis of the non-canonical terpene synthase for 2-methylisoborneol have been studied. Several 2-methylisoborneol synthases have a proline-rich N-terminal domain of unknown function. The results presented here demonstrate that this domain leads to a reduced enzyme activity, in addition to its ability to increase long-term solubility of the protein. Furthermore, the substrate scope of the 2-methylisoborneol synthase was investigated through enzyme incubations with several substrate analogs, giving access to two C12 monoterpenoids. Implications on the stereochemical course of the terpene cyclisation by 2-methylisoborneol synthase are discussed. |
| first_indexed | 2024-03-11T20:31:23Z |
| format | Article |
| id | doaj.art-80e9e47c54e24626a27f0919eaeb19e1 |
| institution | Directory Open Access Journal |
| issn | 1860-5397 |
| language | English |
| last_indexed | 2024-03-11T20:31:23Z |
| publishDate | 2023-09-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Organic Chemistry |
| spelling | doaj.art-80e9e47c54e24626a27f0919eaeb19e12023-10-02T08:42:12ZengBeilstein-InstitutBeilstein Journal of Organic Chemistry1860-53972023-09-011911452145910.3762/bjoc.19.1041860-5397-19-104Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogsBinbin Gu0Lin-Fu Liang1Jeroen S. Dickschat2Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121 Bonn, Germany Two aspects of the biosynthesis of the non-canonical terpene synthase for 2-methylisoborneol have been studied. Several 2-methylisoborneol synthases have a proline-rich N-terminal domain of unknown function. The results presented here demonstrate that this domain leads to a reduced enzyme activity, in addition to its ability to increase long-term solubility of the protein. Furthermore, the substrate scope of the 2-methylisoborneol synthase was investigated through enzyme incubations with several substrate analogs, giving access to two C12 monoterpenoids. Implications on the stereochemical course of the terpene cyclisation by 2-methylisoborneol synthase are discussed.https://doi.org/10.3762/bjoc.19.104biosynthesisenzymesisotopessubstrate analogsterpenes |
| spellingShingle | Binbin Gu Lin-Fu Liang Jeroen S. Dickschat Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs Beilstein Journal of Organic Chemistry biosynthesis enzymes isotopes substrate analogs terpenes |
| title | Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs |
| title_full | Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs |
| title_fullStr | Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs |
| title_full_unstemmed | Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs |
| title_short | Functions of enzyme domains in 2-methylisoborneol biosynthesis and enzymatic synthesis of non-natural analogs |
| title_sort | functions of enzyme domains in 2 methylisoborneol biosynthesis and enzymatic synthesis of non natural analogs |
| topic | biosynthesis enzymes isotopes substrate analogs terpenes |
| url | https://doi.org/10.3762/bjoc.19.104 |
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