Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers
Purpose Fish waste is a protein-rich source that can be used as a value-added product in the formulation of organic liquid fertilizers. This study carried out to investigate the utilizing potential of fruit wastes of Ananas comosus and Carica papaya that contain bromelain and papain as major proteas...
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| Format: | Article |
| Language: | English |
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Islamic Azad University-Isfahan (Khorasgan) Branch- Iran
2021-06-01
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| Series: | International Journal of Recycling of Organic Waste in Agriculture |
| Subjects: | |
| Online Access: | http://ijrowa.khuisf.ac.ir/article_680065_2216ea75001635a383e6712542c0354f.pdf |
| _version_ | 1797329803733368832 |
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| author | RHAA Ranasinghe BTSDP Kannagara RMCS Ratnayake |
| author_facet | RHAA Ranasinghe BTSDP Kannagara RMCS Ratnayake |
| author_sort | RHAA Ranasinghe |
| collection | DOAJ |
| description | Purpose Fish waste is a protein-rich source that can be used as a value-added product in the formulation of organic liquid fertilizers. This study carried out to investigate the utilizing potential of fruit wastes of Ananas comosus and Carica papaya that contain bromelain and papain as major proteases in order to hydrolyze fish waste proteins to fulfill plant nitrogen requirements. Method Proteases were extracted from A. comosus (leaves, crown, unripe fruit pulp, ripe fruit peels and pulp) and C. papaya (leaves, ripe fruit peels and unripe fruit peels). The optimum temperature and pH for the enzymatic activities were determined. Four liquid organic fertilizers were produced with hydrolyzed fish waste that enriched by adding Gliricidia sepium, Chromolaena odorata, Tithonia diversifolia, Mikania scandens and coconut husk-ash. Fertilizers were tested on the growth of Basella alba comparing with a standard fertilizer. Results Ananas comosus ripe fruit peels and the mixture of (A. comosus + C. papaya) showed the highest enzyme activities (0.33±0.02 and 0.36±0.01 U mL-1 enzyme respectively) at 55 oC and 70 oC. The optimum pH for all the studied extracts was 7.5 at 37 oC. The highest plant fresh and dry weights were recorded in the foliar-applied fertilizer produced by hydrolyzing the fish waste using A. comosus and C. papaya, showing no significant differences to the standard fertilizer. Conclusion Hydrolysis of fish waste using the fruit wastes of A. comosus and C. papaya could be effectively used as an organic fertilizer for the growth of B. alba that leads towards sustainable waste management. |
| first_indexed | 2024-03-08T07:10:44Z |
| format | Article |
| id | doaj.art-80ea86e727404d9db9f6d1d7861f2716 |
| institution | Directory Open Access Journal |
| issn | 2195-3228 2251-7715 |
| language | English |
| last_indexed | 2024-03-08T07:10:44Z |
| publishDate | 2021-06-01 |
| publisher | Islamic Azad University-Isfahan (Khorasgan) Branch- Iran |
| record_format | Article |
| series | International Journal of Recycling of Organic Waste in Agriculture |
| spelling | doaj.art-80ea86e727404d9db9f6d1d7861f27162024-02-03T03:07:25ZengIslamic Azad University-Isfahan (Khorasgan) Branch- IranInternational Journal of Recycling of Organic Waste in Agriculture2195-32282251-77152021-06-0110212914310.30486/ijrowa.2021.1891960.1034680065Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizersRHAA Ranasinghe0BTSDP Kannagara1RMCS Ratnayake2Department of Plant and Molecular Biology, Faculty of Science, University of Kelaniya, Sri LankaDepartment of Plant and Molecular Biology, Faculty of Science, University of Kelaniya, Sri LankaDepartment of Plant and Molecular Biology, Faculty of Science, University of Kelaniya, Sri LankaPurpose Fish waste is a protein-rich source that can be used as a value-added product in the formulation of organic liquid fertilizers. This study carried out to investigate the utilizing potential of fruit wastes of Ananas comosus and Carica papaya that contain bromelain and papain as major proteases in order to hydrolyze fish waste proteins to fulfill plant nitrogen requirements. Method Proteases were extracted from A. comosus (leaves, crown, unripe fruit pulp, ripe fruit peels and pulp) and C. papaya (leaves, ripe fruit peels and unripe fruit peels). The optimum temperature and pH for the enzymatic activities were determined. Four liquid organic fertilizers were produced with hydrolyzed fish waste that enriched by adding Gliricidia sepium, Chromolaena odorata, Tithonia diversifolia, Mikania scandens and coconut husk-ash. Fertilizers were tested on the growth of Basella alba comparing with a standard fertilizer. Results Ananas comosus ripe fruit peels and the mixture of (A. comosus + C. papaya) showed the highest enzyme activities (0.33±0.02 and 0.36±0.01 U mL-1 enzyme respectively) at 55 oC and 70 oC. The optimum pH for all the studied extracts was 7.5 at 37 oC. The highest plant fresh and dry weights were recorded in the foliar-applied fertilizer produced by hydrolyzing the fish waste using A. comosus and C. papaya, showing no significant differences to the standard fertilizer. Conclusion Hydrolysis of fish waste using the fruit wastes of A. comosus and C. papaya could be effectively used as an organic fertilizer for the growth of B. alba that leads towards sustainable waste management.http://ijrowa.khuisf.ac.ir/article_680065_2216ea75001635a383e6712542c0354f.pdfananas comosuscarica papayafish wastesplant-derived proteasesorganic liquid fertilizers |
| spellingShingle | RHAA Ranasinghe BTSDP Kannagara RMCS Ratnayake Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers International Journal of Recycling of Organic Waste in Agriculture ananas comosus carica papaya fish wastes plant-derived proteases organic liquid fertilizers |
| title | Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers |
| title_full | Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers |
| title_fullStr | Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers |
| title_full_unstemmed | Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers |
| title_short | Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers |
| title_sort | hydrolysis of fish waste using fruit wastes of ananas comosus and carica papaya for the formulation of liquid fertilizers |
| topic | ananas comosus carica papaya fish wastes plant-derived proteases organic liquid fertilizers |
| url | http://ijrowa.khuisf.ac.ir/article_680065_2216ea75001635a383e6712542c0354f.pdf |
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