Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa
We report here the generation of the human iPSC line INMi005-A from a patient with non-syndromic autosomal recessive retinitis pigmentosa caused by compound heterozygous mutations in the USH2A gene. The reprogramming of primary human dermal fibroblasts was performed using the non-integrative Sendai...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122000873 |
| _version_ | 1818288527945760768 |
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| author | Carla Sanjurjo-Soriano Nejla Erkilic Christel Vache Gregor Dubois Anne-Françoise Roux Isabelle Meunier Vasiliki Kalatzis |
| author_facet | Carla Sanjurjo-Soriano Nejla Erkilic Christel Vache Gregor Dubois Anne-Françoise Roux Isabelle Meunier Vasiliki Kalatzis |
| author_sort | Carla Sanjurjo-Soriano |
| collection | DOAJ |
| description | We report here the generation of the human iPSC line INMi005-A from a patient with non-syndromic autosomal recessive retinitis pigmentosa caused by compound heterozygous mutations in the USH2A gene. The reprogramming of primary human dermal fibroblasts was performed using the non-integrative Sendai virus method and the OSKM transcription factor cocktail. The generated INMi005-A iPSC line is pluripotent and genetically stable, and will represent a valuable tool for understanding the pathophysiology associated with USH2A mutations. |
| first_indexed | 2024-12-13T01:57:48Z |
| format | Article |
| id | doaj.art-80ebaca8f2e7443683df77b81e1672fa |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-13T01:57:48Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-80ebaca8f2e7443683df77b81e1672fa2022-12-22T00:03:20ZengElsevierStem Cell Research1873-50612022-04-0160102738Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosaCarla Sanjurjo-Soriano0Nejla Erkilic1Christel Vache2Gregor Dubois3Anne-Françoise Roux4Isabelle Meunier5Vasiliki Kalatzis6Institute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, France; National Reference Centre for Inherited Sensory Diseases, Univ Montpellier, CHU, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, France; Molecular Genetics Laboratory, Univ Montpellier, CHU Montpellier, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, France; Molecular Genetics Laboratory, Univ Montpellier, CHU Montpellier, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, France; National Reference Centre for Inherited Sensory Diseases, Univ Montpellier, CHU, Montpellier, FranceInstitute for Neurosciences of Montpellier (INM), Univ Montpellier, Inserm, Montpellier, France; Corresponding author at: INM, Inserm U1298, Hopital St Eloi BP 74103, 80 Avenue Augustin Fliche, 34091 Montpellier, France.We report here the generation of the human iPSC line INMi005-A from a patient with non-syndromic autosomal recessive retinitis pigmentosa caused by compound heterozygous mutations in the USH2A gene. The reprogramming of primary human dermal fibroblasts was performed using the non-integrative Sendai virus method and the OSKM transcription factor cocktail. The generated INMi005-A iPSC line is pluripotent and genetically stable, and will represent a valuable tool for understanding the pathophysiology associated with USH2A mutations.http://www.sciencedirect.com/science/article/pii/S1873506122000873 |
| spellingShingle | Carla Sanjurjo-Soriano Nejla Erkilic Christel Vache Gregor Dubois Anne-Françoise Roux Isabelle Meunier Vasiliki Kalatzis Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa Stem Cell Research |
| title | Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa |
| title_full | Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa |
| title_fullStr | Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa |
| title_full_unstemmed | Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa |
| title_short | Generation of a human iPSC line, INMi005-A, from a patient with non-syndromic USH2A-associated retinitis pigmentosa |
| title_sort | generation of a human ipsc line inmi005 a from a patient with non syndromic ush2a associated retinitis pigmentosa |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122000873 |
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