Sensitive imaging of intact microvessels in vivo with synchrotron radiation
Early stages of diseases, including stroke, hypertension, angiogenesis of tumours, spinal cord injuries, etc., are closely associated with the lesions of microvasculature. Rodent models of human vascular diseases are extensively used for the preclinical investigation of the disease evolution and the...
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| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2020-09-01
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| Series: | IUCrJ |
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| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S2052252520008234 |
| _version_ | 1818085615546138624 |
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| author | Feixiang Wang Panting Zhou Ke Li Muyassar Mamtilahun Yaohui Tang Guohao Du Biao Deng Honglan Xie Guoyuan Yang Tiqiao Xiao |
| author_facet | Feixiang Wang Panting Zhou Ke Li Muyassar Mamtilahun Yaohui Tang Guohao Du Biao Deng Honglan Xie Guoyuan Yang Tiqiao Xiao |
| author_sort | Feixiang Wang |
| collection | DOAJ |
| description | Early stages of diseases, including stroke, hypertension, angiogenesis of tumours, spinal cord injuries, etc., are closely associated with the lesions of microvasculature. Rodent models of human vascular diseases are extensively used for the preclinical investigation of the disease evolution and therapy with synchrotron radiation. Therefore, non-invasive and in vivo X-ray imaging with high sensitivity and clarity is desperately needed to visualize the microvessels in live-animal models. Contrast agent is essential for the in vivo X-ray imaging of vessels and angiomatous tissue. Because of the non-rigid motion of adjacent tissues, the short circulation time and the intermittent flow of contrast agents in vessels, it is a great challenge for the traditional X-ray imaging methods to achieve well defined images of microvessels in vivo. In this article, move contrast X-ray imaging (MCXI) based on high-brightness synchrotron radiation is developed to overcome the intrinsic defects in conventional methods. Experiments with live rodents demonstrate the practicability of the MCXI method for sensitive and intact imaging of microvessels in vivo. |
| first_indexed | 2024-12-10T20:12:36Z |
| format | Article |
| id | doaj.art-80ed3a4e3d274a60b7329afe5d697786 |
| institution | Directory Open Access Journal |
| issn | 2052-2525 |
| language | English |
| last_indexed | 2024-12-10T20:12:36Z |
| publishDate | 2020-09-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | IUCrJ |
| spelling | doaj.art-80ed3a4e3d274a60b7329afe5d6977862022-12-22T01:35:16ZengInternational Union of CrystallographyIUCrJ2052-25252020-09-017579380210.1107/S2052252520008234hf5942Sensitive imaging of intact microvessels in vivo with synchrotron radiationFeixiang Wang0Panting Zhou1Ke Li2Muyassar Mamtilahun3Yaohui Tang4Guohao Du5Biao Deng6Honglan Xie7Guoyuan Yang8Tiqiao Xiao9Shanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of ChinaNeuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of ChinaNeuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of ChinaNeuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of ChinaShanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of ChinaShanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of ChinaShanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of ChinaNeuroscience and Neuroengineering Research Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of ChinaShanghai Synchrotron Radiation Facility/Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, People's Republic of ChinaEarly stages of diseases, including stroke, hypertension, angiogenesis of tumours, spinal cord injuries, etc., are closely associated with the lesions of microvasculature. Rodent models of human vascular diseases are extensively used for the preclinical investigation of the disease evolution and therapy with synchrotron radiation. Therefore, non-invasive and in vivo X-ray imaging with high sensitivity and clarity is desperately needed to visualize the microvessels in live-animal models. Contrast agent is essential for the in vivo X-ray imaging of vessels and angiomatous tissue. Because of the non-rigid motion of adjacent tissues, the short circulation time and the intermittent flow of contrast agents in vessels, it is a great challenge for the traditional X-ray imaging methods to achieve well defined images of microvessels in vivo. In this article, move contrast X-ray imaging (MCXI) based on high-brightness synchrotron radiation is developed to overcome the intrinsic defects in conventional methods. Experiments with live rodents demonstrate the practicability of the MCXI method for sensitive and intact imaging of microvessels in vivo.http://scripts.iucr.org/cgi-bin/paper?S2052252520008234microvesselsvascular diseasesmove contrast x-ray imagingin vivo imagingx-ray angiographycontrast agents |
| spellingShingle | Feixiang Wang Panting Zhou Ke Li Muyassar Mamtilahun Yaohui Tang Guohao Du Biao Deng Honglan Xie Guoyuan Yang Tiqiao Xiao Sensitive imaging of intact microvessels in vivo with synchrotron radiation IUCrJ microvessels vascular diseases move contrast x-ray imaging in vivo imaging x-ray angiography contrast agents |
| title | Sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| title_full | Sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| title_fullStr | Sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| title_full_unstemmed | Sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| title_short | Sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| title_sort | sensitive imaging of intact microvessels in vivo with synchrotron radiation |
| topic | microvessels vascular diseases move contrast x-ray imaging in vivo imaging x-ray angiography contrast agents |
| url | http://scripts.iucr.org/cgi-bin/paper?S2052252520008234 |
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