Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis
Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for el...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2016-01-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 |
| _version_ | 1818890358722920448 |
|---|---|
| author | Masaharu Tsuji |
| author_facet | Masaharu Tsuji |
| author_sort | Masaharu Tsuji |
| collection | DOAJ |
| description | Microbes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions. |
| first_indexed | 2024-12-19T17:23:39Z |
| format | Article |
| id | doaj.art-80ee18881baf4eed9b71acde4d944153 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-19T17:23:39Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-80ee18881baf4eed9b71acde4d9441532022-12-21T20:12:37ZengThe Royal SocietyRoyal Society Open Science2054-57032016-01-013710.1098/rsos.160106160106Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopisMasaharu TsujiMicrobes growing at subzero temperatures encounter numerous growth constraints. However, fungi that inhabit cold environments can grow and decompose organic compounds under subzero temperatures. Thus, understanding the cold-adaptation strategies of fungi under extreme environments is critical for elucidating polar-region ecosystems. Here, I report that two strains of the Antarctic basidiomycetous yeast Mrakia blollopis exhibited distinct growth characteristics under subzero conditions: SK-4 grew efficiently, whereas TKG1-2 did not. I analysed the metabolite responses elicited by cold stress in these two M. blollopis strains by using capillary electrophoresis–time-of-flight mass spectrometry. M. blollopis SK-4, which grew well under subzero temperatures, accumulated high levels of TCA-cycle metabolites, lactic acid, aromatic amino acids and polyamines in response to cold shock. Polyamines are recognized to function in cell-growth and developmental processes, and aromatic amino acids are also known to improve cell growth at low temperatures. By contrast, in TKG1-2, which did not grow efficiently, cold stress strongly induced the metabolites of the TCA cycle, but other metabolites were not highly accumulated in the cell. Thus, these differences in metabolite responses could contribute to the distinct abilities of SK-4 and TKG1-2 cells to grow under subzero temperature conditions.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106cold stressbasidiomycetous yeastmetabolite responsecapillary electrophoresis–time-of-flight mass spectrometrymrakia blollopis |
| spellingShingle | Masaharu Tsuji Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis Royal Society Open Science cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis |
| title | Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
| title_full | Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
| title_fullStr | Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
| title_full_unstemmed | Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
| title_short | Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis |
| title_sort | cold stress responses in the antarctic basidiomycetous yeast mrakia blollopis |
| topic | cold stress basidiomycetous yeast metabolite response capillary electrophoresis–time-of-flight mass spectrometry mrakia blollopis |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.160106 |
| work_keys_str_mv | AT masaharutsuji coldstressresponsesintheantarcticbasidiomycetousyeastmrakiablollopis |