Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors
Oxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed reg...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/844 |
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| author | Salah Gariballa Abderrahim Nemmar Ozaz Elzaki Nur Elena Zaaba Javed Yasin |
| author_facet | Salah Gariballa Abderrahim Nemmar Ozaz Elzaki Nur Elena Zaaba Javed Yasin |
| author_sort | Salah Gariballa |
| collection | DOAJ |
| description | Oxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed regarding clinical, anthropometric, and physical activity data at baseline and at 6 months. Blood and urine samples were taken for the measurements of oxidative markers in urine ((glutathione (GSH), thiobarbituric acid reactive substances (TBARS), pteridine, 8-isoprostane and 8-hydroxy-2′-deoxyguanosine (8-OH-dG)), metabolic and inflammatory markers, and related biochemical variables in the blood. Univariate and multiple regression analyses were used to assess the association between oxidative markers and other clinical prognostic indicators. Results: Overall, 168 participants with a complete 6-month follow-up with a mean (±SD) age of 41 ± 12 (119 (71%) females) were included in the study. In multiple regression analysis, log-transformed urinary pteridine levels were significantly correlated with log-transformed urinary GSH, 8-isoprostane, and TBARS after adjusting for urinary creatinine at both baseline and follow-up. Significant correlations were also found between oxidative damage markers and cardiovascular disease risk factors, including systolic blood pressure, HbA1c, plasma glucose, us-C-reactive proteins, total cholesterol, and HDL. Higher TBARS levels were found in males and diabetic subjects, with lower GSH in diabetic hypertensive and obese subjects, but the latter result did not reach statistical significance. We found nonsignificantly higher TBARS, 8-isoprostane, and pteridine levels in smokers compared to those in nonsmokers. All measured urinary oxidative damage markers levels were higher in obese subjects compared with normal-weight subjects, but results did not reach statistical significance. Conclusion: we found significant associations between urinary oxidative damage and metabolic risk factors, and higher levels of urinary oxidative damage markers in diabetic, hypertensive, smoker, and male subjects. |
| first_indexed | 2024-03-10T03:26:24Z |
| format | Article |
| id | doaj.art-80f032d35d1b44b9b80e90181be42cde |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-80f032d35d1b44b9b80e90181be42cde2023-11-23T09:50:39ZengMDPI AGAntioxidants2076-39212022-04-0111584410.3390/antiox11050844Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk FactorsSalah Gariballa0Abderrahim Nemmar1Ozaz Elzaki2Nur Elena Zaaba3Javed Yasin4Internal Medicine, Department of Physiology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab EmiratesInternal Medicine, Department of Physiology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab EmiratesInternal Medicine, Department of Physiology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab EmiratesInternal Medicine, Department of Physiology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab EmiratesInternal Medicine, Department of Physiology, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab EmiratesOxidative damage and inflammation are possible mechanisms linking obesity to diabetes and related complications. This study investigates the levels of oxidative damage markers in the urine of community free-living subjects with increased prevalence of obesity. Methods: Participants were assessed regarding clinical, anthropometric, and physical activity data at baseline and at 6 months. Blood and urine samples were taken for the measurements of oxidative markers in urine ((glutathione (GSH), thiobarbituric acid reactive substances (TBARS), pteridine, 8-isoprostane and 8-hydroxy-2′-deoxyguanosine (8-OH-dG)), metabolic and inflammatory markers, and related biochemical variables in the blood. Univariate and multiple regression analyses were used to assess the association between oxidative markers and other clinical prognostic indicators. Results: Overall, 168 participants with a complete 6-month follow-up with a mean (±SD) age of 41 ± 12 (119 (71%) females) were included in the study. In multiple regression analysis, log-transformed urinary pteridine levels were significantly correlated with log-transformed urinary GSH, 8-isoprostane, and TBARS after adjusting for urinary creatinine at both baseline and follow-up. Significant correlations were also found between oxidative damage markers and cardiovascular disease risk factors, including systolic blood pressure, HbA1c, plasma glucose, us-C-reactive proteins, total cholesterol, and HDL. Higher TBARS levels were found in males and diabetic subjects, with lower GSH in diabetic hypertensive and obese subjects, but the latter result did not reach statistical significance. We found nonsignificantly higher TBARS, 8-isoprostane, and pteridine levels in smokers compared to those in nonsmokers. All measured urinary oxidative damage markers levels were higher in obese subjects compared with normal-weight subjects, but results did not reach statistical significance. Conclusion: we found significant associations between urinary oxidative damage and metabolic risk factors, and higher levels of urinary oxidative damage markers in diabetic, hypertensive, smoker, and male subjects.https://www.mdpi.com/2076-3921/11/5/844urinary oxidative damage markersantioxidantsobesitydiabeteshypertension |
| spellingShingle | Salah Gariballa Abderrahim Nemmar Ozaz Elzaki Nur Elena Zaaba Javed Yasin Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors Antioxidants urinary oxidative damage markers antioxidants obesity diabetes hypertension |
| title | Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors |
| title_full | Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors |
| title_fullStr | Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors |
| title_full_unstemmed | Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors |
| title_short | Urinary Oxidative Damage Markers and Their Association with Obesity-Related Metabolic Risk Factors |
| title_sort | urinary oxidative damage markers and their association with obesity related metabolic risk factors |
| topic | urinary oxidative damage markers antioxidants obesity diabetes hypertension |
| url | https://www.mdpi.com/2076-3921/11/5/844 |
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