The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth
The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old <i>Cladosiphon okamuranus</i>. To determine its...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3274 |
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| author | Yuka Miwa Mahanama Geegana Gamage Awanthi Kouichi Soga Atsuko Tanaka Michihiro Ito Yuichiro Numata Yoichi Sato Teruko Konishi |
| author_facet | Yuka Miwa Mahanama Geegana Gamage Awanthi Kouichi Soga Atsuko Tanaka Michihiro Ito Yuichiro Numata Yoichi Sato Teruko Konishi |
| author_sort | Yuka Miwa |
| collection | DOAJ |
| description | The present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old <i>Cladosiphon okamuranus</i>. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process. |
| first_indexed | 2024-03-10T22:13:25Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:13:25Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-80f199c9b8a44a7e82ea74b010953ec72023-11-19T12:32:33ZengMDPI AGPlants2223-77472023-09-011218327410.3390/plants12183274The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during GrowthYuka Miwa0Mahanama Geegana Gamage Awanthi1Kouichi Soga2Atsuko Tanaka3Michihiro Ito4Yuichiro Numata5Yoichi Sato6Teruko Konishi7Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru, Nishihara-cho 903-0213, Okinawa, JapanUnited Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima-shi 890-0065, Kagoshima, JapanDepartment of Biology, Graduate School of Science, Osaka Metropolitan University, Sugimoto, Sumiyoshi-ku 558-8585, Osaka, JapanDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Senbaru, Nishihara-cho 903-0213, Okinawa, JapanCenter of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus, Senbaru, Nishihara-cho 903-0213, Okinawa, JapanBio-Resources Business Development Division, Riken Food Co., Ltd., Miyauchi, Tagajo-shi 985-0844, Miyagi, JapanBio-Resources Business Development Division, Riken Food Co., Ltd., Miyauchi, Tagajo-shi 985-0844, Miyagi, JapanDepartment of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Senbaru, Nishihara-cho 903-0213, Okinawa, JapanThe present study provides new insights into the growth of the brown algal cell wall by showing that cell wall polysaccharides play an important role in the process of growth, considering the physicochemical characteristic of young and old <i>Cladosiphon okamuranus</i>. To determine its structural variation in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC–I), HC-II, and cellulose, and analyzed physicochemically. Results showed that almost 80% of the total recovery cell wall from both young and old thalli was HW, and HC-I contained mainly fucoidan composed of Fucose, Glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.6~0.7 and 1.0:0.3:0.2~0.3, respectively. Fucoidan in HW was a highly sulfated matrix polysaccharide abundance in young thalli, while fucoidan in HC-I was rich in old thalli and functions as hemicellulose in land plants, crosslinking with cellulose and strengthening the cell wall. We found that HW and HC-I were particularly involved in the growth and strength of old thalli appeared to be due to the deposition of HC-I and the reduction in water content during the growth process.https://www.mdpi.com/2223-7747/12/18/3274Okinawa Mozukucell wall polysaccharidefucoidan |
| spellingShingle | Yuka Miwa Mahanama Geegana Gamage Awanthi Kouichi Soga Atsuko Tanaka Michihiro Ito Yuichiro Numata Yoichi Sato Teruko Konishi The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth Plants Okinawa Mozuku cell wall polysaccharide fucoidan |
| title | The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth |
| title_full | The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth |
| title_fullStr | The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth |
| title_full_unstemmed | The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth |
| title_short | The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth |
| title_sort | cell wall characterization of brown alga i cladosiphon okamuranus i during growth |
| topic | Okinawa Mozuku cell wall polysaccharide fucoidan |
| url | https://www.mdpi.com/2223-7747/12/18/3274 |
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