Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model
PurposeThe aim of this study was to evaluate changes in body weight, liver weight, blood glucose, liver injury markers, pro-inflammatory factors and oxidative stress marker levels in obese mice with HFD induced NAFLD after semaglutide use.Patients and methodsThe 24 C57BL6J mice were randomly divided...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1046130/full |
| _version_ | 1811178398047797248 |
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| author | Shu Niu Shu Niu Shuchun Chen Shuchun Chen Xing Chen Qingjuan Ren Lin Yue Xiaoyu Pan Huiying Zhao Zelin Li Xiaoyi Chen |
| author_facet | Shu Niu Shu Niu Shuchun Chen Shuchun Chen Xing Chen Qingjuan Ren Lin Yue Xiaoyu Pan Huiying Zhao Zelin Li Xiaoyi Chen |
| author_sort | Shu Niu |
| collection | DOAJ |
| description | PurposeThe aim of this study was to evaluate changes in body weight, liver weight, blood glucose, liver injury markers, pro-inflammatory factors and oxidative stress marker levels in obese mice with HFD induced NAFLD after semaglutide use.Patients and methodsThe 24 C57BL6J mice were randomly divided into three groups (NCD, HFD and Sema) for the assessment of metabolic status, inflammatory factor and oxidative stress marker levels, liver histopathology in mice. Liver metabolomics was determined by liquid chromatography/mass spectrometry (LC-MS) method.ResultsThe mice body weight, liver weight, blood glucose, TG, TCHO, LDL and pro-inflammatory factors were significantly reduced after semaglutide. Meanwhile, semaglutide increased the SOD level. Semaglutide treatment significantly improved the pathological changes such as hepatocyte steatosis, balloon degeneration and lymphoid foci by HE. It also significantly reduced lipid droplet by Oil Red O. The mitochondria were swollen, the volume increased, the cristae were partially broken and reduced, the intramembrane matrix was partially dissolved, and the mitophagy structure was visible in the visual field. There were 6 metabolites down-regulated and 2 metabolites significantly up-regulated after semaglutide treatment.ConclusionsSemaglutide can reduce blood glucose level and liver fat accumulation and play an anti-inflammatory role in advanced NAFLD that due to the effects of HFD. |
| first_indexed | 2024-04-11T06:18:37Z |
| format | Article |
| id | doaj.art-80f3ece7ce5b46b6b8a4c24d0add1f39 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T06:18:37Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-80f3ece7ce5b46b6b8a4c24d0add1f392022-12-22T04:40:59ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.10461301046130Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse modelShu Niu0Shu Niu1Shuchun Chen2Shuchun Chen3Xing Chen4Qingjuan Ren5Lin Yue6Xiaoyu Pan7Huiying Zhao8Zelin Li9Xiaoyi Chen10Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, ChinaDepartment of Endocrine, Shijiazhuang People’s Hospital, Shijiazhuang, Hebei, ChinaDepartment of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, ChinaDepartment of Internal Medical, Hebei General Hospital, Shijiazhuang, Hebei, ChinaDepartment of Internal Medical, Hebei General Hospital, Shijiazhuang, Hebei, ChinaDepartment of Endocrine, Shijiazhuang People’s Hospital, Shijiazhuang, Hebei, ChinaDepartment of Endocrine, The Third Hospital of Shijiazhuang, Shijiazhuang, Hebei, ChinaDepartment of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, ChinaDepartment of Endocrine, Shijiazhuang People’s Hospital, Shijiazhuang, Hebei, ChinaDepartment of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, ChinaDepartment of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, ChinaPurposeThe aim of this study was to evaluate changes in body weight, liver weight, blood glucose, liver injury markers, pro-inflammatory factors and oxidative stress marker levels in obese mice with HFD induced NAFLD after semaglutide use.Patients and methodsThe 24 C57BL6J mice were randomly divided into three groups (NCD, HFD and Sema) for the assessment of metabolic status, inflammatory factor and oxidative stress marker levels, liver histopathology in mice. Liver metabolomics was determined by liquid chromatography/mass spectrometry (LC-MS) method.ResultsThe mice body weight, liver weight, blood glucose, TG, TCHO, LDL and pro-inflammatory factors were significantly reduced after semaglutide. Meanwhile, semaglutide increased the SOD level. Semaglutide treatment significantly improved the pathological changes such as hepatocyte steatosis, balloon degeneration and lymphoid foci by HE. It also significantly reduced lipid droplet by Oil Red O. The mitochondria were swollen, the volume increased, the cristae were partially broken and reduced, the intramembrane matrix was partially dissolved, and the mitophagy structure was visible in the visual field. There were 6 metabolites down-regulated and 2 metabolites significantly up-regulated after semaglutide treatment.ConclusionsSemaglutide can reduce blood glucose level and liver fat accumulation and play an anti-inflammatory role in advanced NAFLD that due to the effects of HFD.https://www.frontiersin.org/articles/10.3389/fendo.2022.1046130/fullsemaglutidehepaticNAFLDhepatic fibrosismetabolomics |
| spellingShingle | Shu Niu Shu Niu Shuchun Chen Shuchun Chen Xing Chen Qingjuan Ren Lin Yue Xiaoyu Pan Huiying Zhao Zelin Li Xiaoyi Chen Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model Frontiers in Endocrinology semaglutide hepatic NAFLD hepatic fibrosis metabolomics |
| title | Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model |
| title_full | Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model |
| title_fullStr | Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model |
| title_full_unstemmed | Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model |
| title_short | Semaglutide ameliorates metabolism and hepatic outcomes in an NAFLD mouse model |
| title_sort | semaglutide ameliorates metabolism and hepatic outcomes in an nafld mouse model |
| topic | semaglutide hepatic NAFLD hepatic fibrosis metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1046130/full |
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