Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium
The corneal epithelium, the outermost layer of the cornea, acts as a dynamic barrier preventing access to harmful agents into the intraocular space. It is subjected daily to different insults, and ultraviolet B (UV-B) irradiation represents one of the main causes of injury. In our previous study, we...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/128 |
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| author | Grazia Maugeri Agata Grazia D’Amico Salvatore Giunta Cesarina Giallongo Daniele Tibullo Claudio Bucolo Salvatore Saccone Concetta Federico Davide Scollo Antonio Longo Teresio Avitabile Giuseppe Musumeci Velia D’Agata |
| author_facet | Grazia Maugeri Agata Grazia D’Amico Salvatore Giunta Cesarina Giallongo Daniele Tibullo Claudio Bucolo Salvatore Saccone Concetta Federico Davide Scollo Antonio Longo Teresio Avitabile Giuseppe Musumeci Velia D’Agata |
| author_sort | Grazia Maugeri |
| collection | DOAJ |
| description | The corneal epithelium, the outermost layer of the cornea, acts as a dynamic barrier preventing access to harmful agents into the intraocular space. It is subjected daily to different insults, and ultraviolet B (UV-B) irradiation represents one of the main causes of injury. In our previous study, we demonstrated the beneficial effects of pituitary adenylate cyclase-activating polypeptide (PACAP) against UV-B radiation damage in the human corneal endothelium. Some of its effects are mediated through the activation of the intracellular factor, known as the activity-dependent protein (ADNP). In the present paper, we have investigated the role of ADNP and the small peptide derived from ADNP, known as NAP, in the corneal epithelium. Here, we have demonstrated, for the first time, ADNP expression in human and rabbit corneal epithelium as well as its protective effect by treating the corneal epithelial cells exposed to UV-B radiations with NAP. Our results showed that NAP treatment prevents ROS formation by reducing UV-B-irradiation-induced apoptotic cell death and JNK signalling pathway activation. Further investigations are needed to deeply investigate the possible therapeutic use of NAP to counteract corneal UV-B damage. |
| first_indexed | 2024-03-10T03:00:53Z |
| format | Article |
| id | doaj.art-80faa314bcdc46d586809c1f06952d96 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:00:53Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-80faa314bcdc46d586809c1f06952d962023-11-23T12:47:47ZengMDPI AGAntioxidants2076-39212022-01-0111112810.3390/antiox11010128Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal EpitheliumGrazia Maugeri0Agata Grazia D’Amico1Salvatore Giunta2Cesarina Giallongo3Daniele Tibullo4Claudio Bucolo5Salvatore Saccone6Concetta Federico7Davide Scollo8Antonio Longo9Teresio Avitabile10Giuseppe Musumeci11Velia D’Agata12Section of Anatomy, Histology and Movement Sciences, Department of Biomedical and Biotechnological Sciences, University of Catania, 95100 Catania, ItalyDepartment of Drug Sciences, University of Catania, 95125 Catania, ItalySection of Anatomy, Histology and Movement Sciences, Department of Biomedical and Biotechnological Sciences, University of Catania, 95100 Catania, ItalyDepartment of Medical, Surgical Sciences and Advanced Technologies G.F. Ingrassia, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, Section of Biochemistry, University of Catania, 95123 Catania, ItalyPharmacology Section, Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalySection of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, 95123 Catania, ItalySection of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, 95123 Catania, ItalyDepartment of Ophthalmology, Eye Clinic, University of Catania, 95123 Catania, ItalyCenter for Research in Ocular Pharmacology (CERFO), University of Catania, 95123 Catania, ItalyCenter for Research in Ocular Pharmacology (CERFO), University of Catania, 95123 Catania, ItalySection of Anatomy, Histology and Movement Sciences, Department of Biomedical and Biotechnological Sciences, University of Catania, 95100 Catania, ItalySection of Anatomy, Histology and Movement Sciences, Department of Biomedical and Biotechnological Sciences, University of Catania, 95100 Catania, ItalyThe corneal epithelium, the outermost layer of the cornea, acts as a dynamic barrier preventing access to harmful agents into the intraocular space. It is subjected daily to different insults, and ultraviolet B (UV-B) irradiation represents one of the main causes of injury. In our previous study, we demonstrated the beneficial effects of pituitary adenylate cyclase-activating polypeptide (PACAP) against UV-B radiation damage in the human corneal endothelium. Some of its effects are mediated through the activation of the intracellular factor, known as the activity-dependent protein (ADNP). In the present paper, we have investigated the role of ADNP and the small peptide derived from ADNP, known as NAP, in the corneal epithelium. Here, we have demonstrated, for the first time, ADNP expression in human and rabbit corneal epithelium as well as its protective effect by treating the corneal epithelial cells exposed to UV-B radiations with NAP. Our results showed that NAP treatment prevents ROS formation by reducing UV-B-irradiation-induced apoptotic cell death and JNK signalling pathway activation. Further investigations are needed to deeply investigate the possible therapeutic use of NAP to counteract corneal UV-B damage.https://www.mdpi.com/2076-3921/11/1/128corneal epitheliumUV-BADNPNAPROSapoptosis |
| spellingShingle | Grazia Maugeri Agata Grazia D’Amico Salvatore Giunta Cesarina Giallongo Daniele Tibullo Claudio Bucolo Salvatore Saccone Concetta Federico Davide Scollo Antonio Longo Teresio Avitabile Giuseppe Musumeci Velia D’Agata Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium Antioxidants corneal epithelium UV-B ADNP NAP ROS apoptosis |
| title | Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium |
| title_full | Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium |
| title_fullStr | Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium |
| title_full_unstemmed | Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium |
| title_short | Activity-Dependent Neuroprotective Protein (ADNP)-Derived Peptide (NAP) Counteracts UV-B Radiation-Induced ROS Formation in Corneal Epithelium |
| title_sort | activity dependent neuroprotective protein adnp derived peptide nap counteracts uv b radiation induced ros formation in corneal epithelium |
| topic | corneal epithelium UV-B ADNP NAP ROS apoptosis |
| url | https://www.mdpi.com/2076-3921/11/1/128 |
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