Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice
Purpose. To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome P450 reductase (CPR). Methods. The inhibitory effects of star fruit juice (0.5 to 5%, v/v) against the activities of seven CYP isoforms includin...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2007-10-01
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| Series: | Journal of Pharmacy & Pharmaceutical Sciences |
| Online Access: | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/776 |
| _version_ | 1797725475588538368 |
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| author | Jiang-Wei Zhang Yong Liu Jie Cheng Wei Li Hong Ma Hong-Tao Liu Jie Sun Li-Ming Wang Yu-Qi He Yun Wang Zheng-Tao Wang Ling Yang |
| author_facet | Jiang-Wei Zhang Yong Liu Jie Cheng Wei Li Hong Ma Hong-Tao Liu Jie Sun Li-Ming Wang Yu-Qi He Yun Wang Zheng-Tao Wang Ling Yang |
| author_sort | Jiang-Wei Zhang |
| collection | DOAJ |
| description | Purpose. To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome P450 reductase (CPR). Methods. The inhibitory effects of star fruit juice (0.5 to 5%, v/v) against the activities of seven CYP isoforms including CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4 and CPR were examined in human liver microsomes. To identify time-dependent inhibition, star fruit juice (2.5%, v/v) was preincubated with microsomes and a NADPH-generating system for 0-15 min, and then the extent of inhibition towards seven CYP isoforms were examined. Results. Star fruit juice (5.0%, v/v) was found to inhibit all the activities of CYP isoforms tested by more than 70%. Based on the half inhibition values (%, v/v), the inhibitory effects towards different CYP isoforms were in the following order: CYP2A6 (0.9) > CYP1A2 (1.4) > CYP2D6 (1.6) > CYP2E1 (2.0) > CYP2C8 (2.2) > CYP2C9 (3.0) > CYP3A4 (3.2). Time-dependent inhibition was not observed towards any of the tested CYP isoforms. In addition, star fruit juice was found not to inhibit the activity of CPR. Conclusions. Star fruit juice inhibited the seven CYP isoforms tested, with the strongest inhibitory effect against CYP2A6 and the least towards CYP3A4. |
| first_indexed | 2024-03-12T10:31:48Z |
| format | Article |
| id | doaj.art-80fc001a504f4ce5a04ff6b4d138eb77 |
| institution | Directory Open Access Journal |
| issn | 1482-1826 |
| language | English |
| last_indexed | 2024-03-12T10:31:48Z |
| publishDate | 2007-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmaceutical Sciences |
| spelling | doaj.art-80fc001a504f4ce5a04ff6b4d138eb772023-09-02T09:10:00ZengFrontiers Media S.A.Journal of Pharmacy & Pharmaceutical Sciences1482-18262007-10-0110410.18433/J30593Inhibition of Human Liver Cytochrome P450 by Star Fruit JuiceJiang-Wei Zhang0Yong Liu1Jie Cheng2Wei Li3Hong Ma4Hong-Tao Liu5Jie Sun6Li-Ming Wang7Yu-Qi He8Yun Wang9Zheng-Tao Wang10Ling Yang11Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaLaboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, ChinaThe Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaThe Second Affiliated Hospital of Dalian Medical University, Dalian, ChinaUniversity of Traditional Chinese Medicine, Shanghai, ChinaUniversity of Traditional Chinese Medicine, Shanghai, ChinaUniversity of Traditional Chinese Medicine, Shanghai, ChinaInstitute of Chemical PhysicsPurpose. To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome P450 reductase (CPR). Methods. The inhibitory effects of star fruit juice (0.5 to 5%, v/v) against the activities of seven CYP isoforms including CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4 and CPR were examined in human liver microsomes. To identify time-dependent inhibition, star fruit juice (2.5%, v/v) was preincubated with microsomes and a NADPH-generating system for 0-15 min, and then the extent of inhibition towards seven CYP isoforms were examined. Results. Star fruit juice (5.0%, v/v) was found to inhibit all the activities of CYP isoforms tested by more than 70%. Based on the half inhibition values (%, v/v), the inhibitory effects towards different CYP isoforms were in the following order: CYP2A6 (0.9) > CYP1A2 (1.4) > CYP2D6 (1.6) > CYP2E1 (2.0) > CYP2C8 (2.2) > CYP2C9 (3.0) > CYP3A4 (3.2). Time-dependent inhibition was not observed towards any of the tested CYP isoforms. In addition, star fruit juice was found not to inhibit the activity of CPR. Conclusions. Star fruit juice inhibited the seven CYP isoforms tested, with the strongest inhibitory effect against CYP2A6 and the least towards CYP3A4.https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/776 |
| spellingShingle | Jiang-Wei Zhang Yong Liu Jie Cheng Wei Li Hong Ma Hong-Tao Liu Jie Sun Li-Ming Wang Yu-Qi He Yun Wang Zheng-Tao Wang Ling Yang Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice Journal of Pharmacy & Pharmaceutical Sciences |
| title | Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice |
| title_full | Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice |
| title_fullStr | Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice |
| title_full_unstemmed | Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice |
| title_short | Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice |
| title_sort | inhibition of human liver cytochrome p450 by star fruit juice |
| url | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/776 |
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