Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures
The article presents in retrospect the methods of cryopreservation of sperm of bulls and breeding rams. It is indicated that the first methods were proposed by I.V. Smirnov in 1947–1951, which ensured the production of viable lambs and calves. The significant contribution to the development of the m...
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| Format: | Article |
| Language: | English |
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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
2020-10-01
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| Series: | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Гжицького. Серія: Сільськогосподарські науки |
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| Online Access: | https://nvlvet.com.ua/index.php/agriculture/article/view/4042 |
| _version_ | 1811270873672318976 |
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| author | K. Hrymak |
| author_facet | K. Hrymak |
| author_sort | K. Hrymak |
| collection | DOAJ |
| description | The article presents in retrospect the methods of cryopreservation of sperm of bulls and breeding rams. It is indicated that the first methods were proposed by I.V. Smirnov in 1947–1951, which ensured the production of viable lambs and calves. The significant contribution to the development of the method of cryopreservation of bull sperm by British scientists Polge C. and Rawson L. and Japanese scientists Nagase H. and Niwa T. The methods developed by them, with minor changes, have been used by scientists and breeding specialists for many decades. Modern technologies of cryopreservation of bull sperm are represented, in particular: Kharkiv in coated granules (author FI Ostashko and others), French company IMV-R-Cassou, German company Minitub, which are currently used in most countries with developed animal husbandry. It is noted that the first attempts to freeze the sperm of breeding rams were made by E. M. Platov in 1965. It is noted that in the cryopreservation of sperm the importance of adaptation and equilibration processes is important. The role of glycerin as a cryoprotectant in freezing the semen of male farm animals has been elucidated. The optimal concentration of glycerin in the media for cryopreservation of sperm of fetuses has been established. It is noted that the basis of “temperature shock” of sperm is damage to the sperm membrane, which is caused by balancing the concentration gradient that occurs at the boundary of cell-medium division with a sharp change in temperature. A high correlation between the physiological quality of the acrosome of sperm and its fertilizing ability is indicated. Studies of the chemical composition of the sperm of breeding rams have shown that it differs from the sperm of other animal species by a slightly higher content of proteins, lipids of lactic acid and a lower concentration of minerals. Proteins contain much more amino acids. Sheep sperm do not tolerate aerobic conditions, it is proved that damage to sperm by oxygen occurs mainly after its dilution and equilibration, and not during cryopreservation. Under aerobic conditions, one amino acid is cleaved from the molecule of lecithin, which is part of the membranes of sperm, with the formation of toxic lysolecithin. The most critical zones at thawing of deep-frozen sperm are shown. It has been experimentally established that the rate of the recrystallization process can be reduced by using cryoprotectants in environments, as well as by increasing the rate of thawing. An important influence in the thawing of sperm has the selection of the optimal mode of thawing. Analysis of literature data revealed that in practical conditions in the process of freezing ‒ thawing about 50% of sperm lose activity, which indicates that currently available methods of freezing sperm of breeding rams are imperfect and need further study and improvement. |
| first_indexed | 2024-04-12T22:10:51Z |
| format | Article |
| id | doaj.art-80fc5e7a14e64571aa2c161fc85b8b0d |
| institution | Directory Open Access Journal |
| issn | 2519-2698 2707-5834 |
| language | English |
| last_indexed | 2024-04-12T22:10:51Z |
| publishDate | 2020-10-01 |
| publisher | Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv |
| record_format | Article |
| series | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Гжицького. Серія: Сільськогосподарські науки |
| spelling | doaj.art-80fc5e7a14e64571aa2c161fc85b8b0d2022-12-22T03:14:46ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivНауковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Гжицького. Серія: Сільськогосподарські науки2519-26982707-58342020-10-01229310211210.32718/nvlvet-a93184042Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperaturesK. Hrymak0Institute of Animal Biology NAAS, Lviv, UkraineThe article presents in retrospect the methods of cryopreservation of sperm of bulls and breeding rams. It is indicated that the first methods were proposed by I.V. Smirnov in 1947–1951, which ensured the production of viable lambs and calves. The significant contribution to the development of the method of cryopreservation of bull sperm by British scientists Polge C. and Rawson L. and Japanese scientists Nagase H. and Niwa T. The methods developed by them, with minor changes, have been used by scientists and breeding specialists for many decades. Modern technologies of cryopreservation of bull sperm are represented, in particular: Kharkiv in coated granules (author FI Ostashko and others), French company IMV-R-Cassou, German company Minitub, which are currently used in most countries with developed animal husbandry. It is noted that the first attempts to freeze the sperm of breeding rams were made by E. M. Platov in 1965. It is noted that in the cryopreservation of sperm the importance of adaptation and equilibration processes is important. The role of glycerin as a cryoprotectant in freezing the semen of male farm animals has been elucidated. The optimal concentration of glycerin in the media for cryopreservation of sperm of fetuses has been established. It is noted that the basis of “temperature shock” of sperm is damage to the sperm membrane, which is caused by balancing the concentration gradient that occurs at the boundary of cell-medium division with a sharp change in temperature. A high correlation between the physiological quality of the acrosome of sperm and its fertilizing ability is indicated. Studies of the chemical composition of the sperm of breeding rams have shown that it differs from the sperm of other animal species by a slightly higher content of proteins, lipids of lactic acid and a lower concentration of minerals. Proteins contain much more amino acids. Sheep sperm do not tolerate aerobic conditions, it is proved that damage to sperm by oxygen occurs mainly after its dilution and equilibration, and not during cryopreservation. Under aerobic conditions, one amino acid is cleaved from the molecule of lecithin, which is part of the membranes of sperm, with the formation of toxic lysolecithin. The most critical zones at thawing of deep-frozen sperm are shown. It has been experimentally established that the rate of the recrystallization process can be reduced by using cryoprotectants in environments, as well as by increasing the rate of thawing. An important influence in the thawing of sperm has the selection of the optimal mode of thawing. Analysis of literature data revealed that in practical conditions in the process of freezing ‒ thawing about 50% of sperm lose activity, which indicates that currently available methods of freezing sperm of breeding rams are imperfect and need further study and improvement.https://nvlvet.com.ua/index.php/agriculture/article/view/4042breeding rams, semen, cryopreservation, thawing, equilibration, cryoprotectants, fertilizing ability. |
| spellingShingle | K. Hrymak Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Гжицького. Серія: Сільськогосподарські науки breeding rams, semen, cryopreservation, thawing, equilibration, cryoprotectants, fertilizing ability. |
| title | Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures |
| title_full | Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures |
| title_fullStr | Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures |
| title_full_unstemmed | Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures |
| title_short | Cryopreservation of ram-sires semen and its qualitative changes under the influence of low temperatures |
| title_sort | cryopreservation of ram sires semen and its qualitative changes under the influence of low temperatures |
| topic | breeding rams, semen, cryopreservation, thawing, equilibration, cryoprotectants, fertilizing ability. |
| url | https://nvlvet.com.ua/index.php/agriculture/article/view/4042 |
| work_keys_str_mv | AT khrymak cryopreservationoframsiressemenanditsqualitativechangesundertheinfluenceoflowtemperatures |