Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions

Ultrasound-assisted solvent extraction (UAE) was applied to extract underutilized Madhuca longifolia seed oil. The effect of extraction time, temperature, solvent type, solvent/sample ratio, and amplitude on the oil yield and recovery were investigated. Approximately 56.97% of oil yield and 99.54% o...

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Main Authors: R.C.N. Thilakarathna, Lee Fong Siow, Teck-Kim Tang, Eng-Seng Chan, Yee-Ying Lee
Format: Article
Language:English
Published: Elsevier 2023-01-01
Series:Ultrasonics Sonochemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1350417722003765
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author R.C.N. Thilakarathna
Lee Fong Siow
Teck-Kim Tang
Eng-Seng Chan
Yee-Ying Lee
author_facet R.C.N. Thilakarathna
Lee Fong Siow
Teck-Kim Tang
Eng-Seng Chan
Yee-Ying Lee
author_sort R.C.N. Thilakarathna
collection DOAJ
description Ultrasound-assisted solvent extraction (UAE) was applied to extract underutilized Madhuca longifolia seed oil. The effect of extraction time, temperature, solvent type, solvent/sample ratio, and amplitude on the oil yield and recovery were investigated. Approximately 56.97% of oil yield and 99.54% of oil recovery were attained using mild conditions of 35 min, 35 °C, 40% amplitude, isopropanol to acetone (1:1), and solvent to sample (20 mL/g). UAE oil yield and recovery were comparable with Soxhlet extraction (SXE) whilst mechanical pressing (ME) yielded < 50% of UAE recovery. UAE does not affect the fatty acids composition (46% C18:1; 22% C16:0; 21% C18:0, 10% C18:2), and triacylglycerol profile (23% POO, 17% POS, 16% SOO, and 14% POP). Interestingly, UAE extracted oil conferred remarkably (P < 0.05) higher antioxidant capacity (IC50 of DPPH 106.60 mg/mL and ABTS 39.80 mg/mL) than SXE (IC50 of DPPH 810.40 mg/mL and ABTS 757.43 mg/mL) or ME (IC50 of DPPH 622.38 mg/mL and ABTS 392.87 mg/mL).
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spelling doaj.art-80fe0c390a4048bebf6a783bf52d2ba32023-01-11T04:27:58ZengElsevierUltrasonics Sonochemistry1350-41772023-01-0192106280Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractionsR.C.N. Thilakarathna0Lee Fong Siow1Teck-Kim Tang2Eng-Seng Chan3Yee-Ying Lee4School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, MalaysiaSchool of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, MalaysiaInstitute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, MalaysiaChemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia; Monash-Industry Plant Oils Research Laboratory, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, MalaysiaSchool of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia; Monash-Industry Plant Oils Research Laboratory, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia; Corresponding author at: School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia.Ultrasound-assisted solvent extraction (UAE) was applied to extract underutilized Madhuca longifolia seed oil. The effect of extraction time, temperature, solvent type, solvent/sample ratio, and amplitude on the oil yield and recovery were investigated. Approximately 56.97% of oil yield and 99.54% of oil recovery were attained using mild conditions of 35 min, 35 °C, 40% amplitude, isopropanol to acetone (1:1), and solvent to sample (20 mL/g). UAE oil yield and recovery were comparable with Soxhlet extraction (SXE) whilst mechanical pressing (ME) yielded < 50% of UAE recovery. UAE does not affect the fatty acids composition (46% C18:1; 22% C16:0; 21% C18:0, 10% C18:2), and triacylglycerol profile (23% POO, 17% POS, 16% SOO, and 14% POP). Interestingly, UAE extracted oil conferred remarkably (P < 0.05) higher antioxidant capacity (IC50 of DPPH 106.60 mg/mL and ABTS 39.80 mg/mL) than SXE (IC50 of DPPH 810.40 mg/mL and ABTS 757.43 mg/mL) or ME (IC50 of DPPH 622.38 mg/mL and ABTS 392.87 mg/mL).http://www.sciencedirect.com/science/article/pii/S1350417722003765Advanced oil extractionMahua oilUltrasoundUnderutilizedAntioxidantSeed
spellingShingle R.C.N. Thilakarathna
Lee Fong Siow
Teck-Kim Tang
Eng-Seng Chan
Yee-Ying Lee
Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
Ultrasonics Sonochemistry
Advanced oil extraction
Mahua oil
Ultrasound
Underutilized
Antioxidant
Seed
title Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
title_full Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
title_fullStr Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
title_full_unstemmed Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
title_short Physicochemical and antioxidative properties of ultrasound-assisted extraction of mahua (Madhuca longifolia) seed oil in comparison with conventional Soxhlet and mechanical extractions
title_sort physicochemical and antioxidative properties of ultrasound assisted extraction of mahua madhuca longifolia seed oil in comparison with conventional soxhlet and mechanical extractions
topic Advanced oil extraction
Mahua oil
Ultrasound
Underutilized
Antioxidant
Seed
url http://www.sciencedirect.com/science/article/pii/S1350417722003765
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