A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis
Stearoyl-CoA desaturase (SCD) catalyzes the de novo biosynthesis of oleate and palmitoleate, which are the major fatty acids found in triglycerides, cholesteryl esters, and phospholipids. A high carbohydrate (lipogenic) diet induces lipogenic gene expression by sterol regulatory element binding prot...
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| Format: | Article |
| Language: | English |
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Elsevier
2001-07-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520315893 |
| _version_ | 1818602484919173120 |
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| author | Makoto Miyazaki Young-Cheul Kim James M. Ntambi |
| author_facet | Makoto Miyazaki Young-Cheul Kim James M. Ntambi |
| author_sort | Makoto Miyazaki |
| collection | DOAJ |
| description | Stearoyl-CoA desaturase (SCD) catalyzes the de novo biosynthesis of oleate and palmitoleate, which are the major fatty acids found in triglycerides, cholesteryl esters, and phospholipids. A high carbohydrate (lipogenic) diet induces lipogenic gene expression by sterol regulatory element binding protein 1 (SREBP-1c)-mediated gene transcription, leading to an increase in the synthesis of triglycerides. The lipogenic diet fed to mice with a null mutation in the SCD1 gene (SCD −/−) fails to induce the synthesis of triglycerides in liver, despite the induction of expression of SREBP-1 and its target genes, fatty acid synthase and glycerol-3-phosphate acyltransferase. The lipogenic diet led to a decrease in the levels of triglyceride, but an increase in the level of cholesteryl esters of saturated fatty acids. Feeding a lipogenic diet supplemented with high levels of oleate to the SCD −/− mice resulted in incorporation of oleate in the liver of SCD −/− mice, but failed to restore triglycerides to the levels in the normal mouse.Triglyceride synthesis, as measured by the incorporation of [3H]glycerol, was dramatically reduced in the liver of SCD −/− mouse fed a lipogenic diet compared with the normal mouse. These observations demonstrate that induction of triglyceride synthesis is highly dependent on SCD1 gene expression.—Miyazaki, M., Y-C. Kim, and J. M. Ntambi. A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis. J. Lipid Res. 2001. 42: 1018–1024. |
| first_indexed | 2024-12-16T13:08:01Z |
| format | Article |
| id | doaj.art-80fe351a25934454be452fa31b72c1c6 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T13:08:01Z |
| publishDate | 2001-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-80fe351a25934454be452fa31b72c1c62022-12-21T22:30:41ZengElsevierJournal of Lipid Research0022-22752001-07-0142710181024A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesisMakoto Miyazaki0Young-Cheul Kim1James M. Ntambi2Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706To whom correspondence should be addressed; Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706; Department of Nutritional Sciences, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706Stearoyl-CoA desaturase (SCD) catalyzes the de novo biosynthesis of oleate and palmitoleate, which are the major fatty acids found in triglycerides, cholesteryl esters, and phospholipids. A high carbohydrate (lipogenic) diet induces lipogenic gene expression by sterol regulatory element binding protein 1 (SREBP-1c)-mediated gene transcription, leading to an increase in the synthesis of triglycerides. The lipogenic diet fed to mice with a null mutation in the SCD1 gene (SCD −/−) fails to induce the synthesis of triglycerides in liver, despite the induction of expression of SREBP-1 and its target genes, fatty acid synthase and glycerol-3-phosphate acyltransferase. The lipogenic diet led to a decrease in the levels of triglyceride, but an increase in the level of cholesteryl esters of saturated fatty acids. Feeding a lipogenic diet supplemented with high levels of oleate to the SCD −/− mice resulted in incorporation of oleate in the liver of SCD −/− mice, but failed to restore triglycerides to the levels in the normal mouse.Triglyceride synthesis, as measured by the incorporation of [3H]glycerol, was dramatically reduced in the liver of SCD −/− mouse fed a lipogenic diet compared with the normal mouse. These observations demonstrate that induction of triglyceride synthesis is highly dependent on SCD1 gene expression.—Miyazaki, M., Y-C. Kim, and J. M. Ntambi. A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis. J. Lipid Res. 2001. 42: 1018–1024.http://www.sciencedirect.com/science/article/pii/S0022227520315893triglyceridescholesteryl estersgene expressionSREBP |
| spellingShingle | Makoto Miyazaki Young-Cheul Kim James M. Ntambi A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis Journal of Lipid Research triglycerides cholesteryl esters gene expression SREBP |
| title | A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| title_full | A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| title_fullStr | A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| title_full_unstemmed | A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| title_short | A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| title_sort | lipogenic diet in mice with a disruption of the stearoyl coa desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis |
| topic | triglycerides cholesteryl esters gene expression SREBP |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520315893 |
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