Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry
The control of <i>Campylobacter</i> in poultry at the pre-harvest level is critical to reducing foodborne infections with <i>Campylobacter</i> since the consumption of contaminated poultry is the most frequent cause of human campylobacteriosis. Although poultry vaccination is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/5/685 |
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| author | Byeonghwa Jeon Tunchanok Saisom Jiroj Sasipreeyajan Taradon Luangtongkum |
| author_facet | Byeonghwa Jeon Tunchanok Saisom Jiroj Sasipreeyajan Taradon Luangtongkum |
| author_sort | Byeonghwa Jeon |
| collection | DOAJ |
| description | The control of <i>Campylobacter</i> in poultry at the pre-harvest level is critical to reducing foodborne infections with <i>Campylobacter</i> since the consumption of contaminated poultry is the most frequent cause of human campylobacteriosis. Although poultry vaccination is suggested as useful intervention measures, no <i>Campylobacter</i> vaccines are currently available. To develop live-attenuated oral <i>Campylobacter</i> vaccines, in this study, we evaluated the efficacy of pre-colonization by oxidative stress defense mutants, including knockout mutants of <i>ahpC</i>, <i>katA</i>, and <i>sodB</i>, in preventing <i>Campylobacter jejuni</i> from colonizing poultry. Interestingly, when chickens were pre-colonized with <i>ΔahpC</i> and <i>ΔkatA</i> mutants, rather than the <i>ΔsodB</i> mutant, the level of <i>C. jejuni</i> colonization was significantly reduced within 35 days. Further studies demonstrated when chickens were pre-colonized with the <i>ΔahpC</i> mutant by oral challenge with a high dose (ca., 5 × 10<sup>8</sup> CFU/bird) and a low dose (ca., 5 × 10<sup>6</sup> CFU/bird), it twice reduced the level of <i>C. jejuni</i> by 3.9 log<sub>10</sub>CFU/g feces and 3 log<sub>10</sub>CFU/g feces after 42 days, respectively, compared to the untreated control. Due to a colonization defect, the <i>ΔahpC</i> mutant was removed from chickens within 42 days. After excretion from the host, moreover, the <i>ΔahpC</i> mutant cannot survive in aerobic environments because of compromised aerotolerance. Our findings suggest that the <i>ahpC</i> mutant has a great potential for on-farm application to control <i>C. jejuni</i> at the pre-harvest level. |
| first_indexed | 2024-03-10T01:40:27Z |
| format | Article |
| id | doaj.art-80ff330592c84b89ba9b7a0aab9a4e26 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T01:40:27Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-80ff330592c84b89ba9b7a0aab9a4e262023-11-23T13:25:30ZengMDPI AGVaccines2076-393X2022-04-0110568510.3390/vaccines10050685Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in PoultryByeonghwa Jeon0Tunchanok Saisom1Jiroj Sasipreeyajan2Taradon Luangtongkum3School of Public Health, University of Alberta, Edmonton, AB T6G 2R3, CanadaDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandThe control of <i>Campylobacter</i> in poultry at the pre-harvest level is critical to reducing foodborne infections with <i>Campylobacter</i> since the consumption of contaminated poultry is the most frequent cause of human campylobacteriosis. Although poultry vaccination is suggested as useful intervention measures, no <i>Campylobacter</i> vaccines are currently available. To develop live-attenuated oral <i>Campylobacter</i> vaccines, in this study, we evaluated the efficacy of pre-colonization by oxidative stress defense mutants, including knockout mutants of <i>ahpC</i>, <i>katA</i>, and <i>sodB</i>, in preventing <i>Campylobacter jejuni</i> from colonizing poultry. Interestingly, when chickens were pre-colonized with <i>ΔahpC</i> and <i>ΔkatA</i> mutants, rather than the <i>ΔsodB</i> mutant, the level of <i>C. jejuni</i> colonization was significantly reduced within 35 days. Further studies demonstrated when chickens were pre-colonized with the <i>ΔahpC</i> mutant by oral challenge with a high dose (ca., 5 × 10<sup>8</sup> CFU/bird) and a low dose (ca., 5 × 10<sup>6</sup> CFU/bird), it twice reduced the level of <i>C. jejuni</i> by 3.9 log<sub>10</sub>CFU/g feces and 3 log<sub>10</sub>CFU/g feces after 42 days, respectively, compared to the untreated control. Due to a colonization defect, the <i>ΔahpC</i> mutant was removed from chickens within 42 days. After excretion from the host, moreover, the <i>ΔahpC</i> mutant cannot survive in aerobic environments because of compromised aerotolerance. Our findings suggest that the <i>ahpC</i> mutant has a great potential for on-farm application to control <i>C. jejuni</i> at the pre-harvest level.https://www.mdpi.com/2076-393X/10/5/685<i>Campylobacter</i>live-attenuated oral vaccinespoultryfood safety |
| spellingShingle | Byeonghwa Jeon Tunchanok Saisom Jiroj Sasipreeyajan Taradon Luangtongkum Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry Vaccines <i>Campylobacter</i> live-attenuated oral vaccines poultry food safety |
| title | Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry |
| title_full | Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry |
| title_fullStr | Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry |
| title_full_unstemmed | Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry |
| title_short | Live-Attenuated Oral Vaccines to Reduce <i>Campylobacter</i> Colonization in Poultry |
| title_sort | live attenuated oral vaccines to reduce i campylobacter i colonization in poultry |
| topic | <i>Campylobacter</i> live-attenuated oral vaccines poultry food safety |
| url | https://www.mdpi.com/2076-393X/10/5/685 |
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