Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice
Background & aim: The formation of skin scars is inevitable following surgery, trauma and burns. Wound healing is a complex process that occurs during the competition of collagen production and collapse mechanisms. Various factors such as diabetes can interfere this process with multiple mecha...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2021-02-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/article-1-2529-en.html |
| _version_ | 1818018862146256896 |
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| author | A Taheri Akardi MM Fattahi SH epehrtaj Z Ghahremani SH Pabdar Z Shayan |
| author_facet | A Taheri Akardi MM Fattahi SH epehrtaj Z Ghahremani SH Pabdar Z Shayan |
| author_sort | A Taheri Akardi |
| collection | DOAJ |
| description | Background & aim: The formation of skin scars is inevitable following surgery, trauma and burns. Wound healing is a complex process that occurs during the competition of collagen production and collapse mechanisms. Various factors such as diabetes can interfere this process with multiple mechanisms. There are also ways to speed up the process of repair and prevent wound formation which is the use of light. The present study examines the effect of polarizing light (bioptron) on the process of wound healing and prevention of wound formation in diabetic and non-diabetic rats.
Methods: A total of 52 rats were studied in four groups of 13 with and without diabetes and with and without using light bioptron: First group: without diabetes, without light; group 2: without diabetes, with light; group 3: with diabetes, without light; group 4: with diabetes, with light. At first, the wound was formed about 2 cm in rats and sutured. Then grouping wound was dressed with normal saline daily in two groups with and without diabetes and the other group was exposed to bipotron light at a distance of 10 cm for 10 minutes with a power of 2.4 joule cm2. Then the scars were scanned by using the Stony Brook Scar Scale for objective measurements of the wound over a period of 21 days. In the end, the rats were sacrificed and the wounds were sent to the lab for histological examination. The incidence of wound was studied based on histological examination on necrosis parameters, fibroblastic proliferation, collagen accumulation, granulation tissue maturation, epithelialization and neovascularization.
Results: There was no significant difference between the two groups in terms of SBSES scores on days 7, 14 and 21. There was no difference in histopathological characteristics except for non-diabetic rats that bioptron were reduced fibroblast proliferation(pvalue<0.001) and decreased neovascularization(pvalue<0.001).
Conclusion: Despite the lack of significant differences between groups, using of polarized light can be corrected by reducing the proliferation of fibroblasts in the wound healing and reducing neovascularization to improve the appearance of the scar and can be used as a safe method. |
| first_indexed | 2024-04-14T07:44:20Z |
| format | Article |
| id | doaj.art-80fff700db19404ca85a09170c47f2b7 |
| institution | Directory Open Access Journal |
| issn | 1728-6514 1728-6514 |
| language | fas |
| last_indexed | 2024-04-14T07:44:20Z |
| publishDate | 2021-02-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-80fff700db19404ca85a09170c47f2b72022-12-22T02:05:23ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65141728-65142021-02-01251111Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic MiceA Taheri Akardi0MM Fattahi1SH epehrtaj2Z Ghahremani3SH Pabdar4Z Shayan5 Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Surgery Groups, Shiraz University of Medical Sciences, Shiraz, Iran Surgery Groups, Shiraz University of Medical Sciences, Shiraz, Iran Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Background & aim: The formation of skin scars is inevitable following surgery, trauma and burns. Wound healing is a complex process that occurs during the competition of collagen production and collapse mechanisms. Various factors such as diabetes can interfere this process with multiple mechanisms. There are also ways to speed up the process of repair and prevent wound formation which is the use of light. The present study examines the effect of polarizing light (bioptron) on the process of wound healing and prevention of wound formation in diabetic and non-diabetic rats. Methods: A total of 52 rats were studied in four groups of 13 with and without diabetes and with and without using light bioptron: First group: without diabetes, without light; group 2: without diabetes, with light; group 3: with diabetes, without light; group 4: with diabetes, with light. At first, the wound was formed about 2 cm in rats and sutured. Then grouping wound was dressed with normal saline daily in two groups with and without diabetes and the other group was exposed to bipotron light at a distance of 10 cm for 10 minutes with a power of 2.4 joule cm2. Then the scars were scanned by using the Stony Brook Scar Scale for objective measurements of the wound over a period of 21 days. In the end, the rats were sacrificed and the wounds were sent to the lab for histological examination. The incidence of wound was studied based on histological examination on necrosis parameters, fibroblastic proliferation, collagen accumulation, granulation tissue maturation, epithelialization and neovascularization. Results: There was no significant difference between the two groups in terms of SBSES scores on days 7, 14 and 21. There was no difference in histopathological characteristics except for non-diabetic rats that bioptron were reduced fibroblast proliferation(pvalue<0.001) and decreased neovascularization(pvalue<0.001). Conclusion: Despite the lack of significant differences between groups, using of polarized light can be corrected by reducing the proliferation of fibroblasts in the wound healing and reducing neovascularization to improve the appearance of the scar and can be used as a safe method.http://armaghanj.yums.ac.ir/article-1-2529-en.htmlwound healingcicatrixlightphototherapylow-level light therapy |
| spellingShingle | A Taheri Akardi MM Fattahi SH epehrtaj Z Ghahremani SH Pabdar Z Shayan Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice Armaghane Danesh Bimonthly Journal wound healing cicatrix light phototherapy low-level light therapy |
| title | Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice |
| title_full | Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice |
| title_fullStr | Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice |
| title_full_unstemmed | Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice |
| title_short | Investigation of the Effect of Bioptron Light in Preventing the Formation of Scars in Diabetic and Non-Diabetic Mice |
| title_sort | investigation of the effect of bioptron light in preventing the formation of scars in diabetic and non diabetic mice |
| topic | wound healing cicatrix light phototherapy low-level light therapy |
| url | http://armaghanj.yums.ac.ir/article-1-2529-en.html |
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