Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients
The World Health Organization in 2017 listed carbapenem resistant Enterobacteriaceae (CRE) with critical priority for research. A research to assess carbapenem resistant Escherichia coli (CREc) in coliuria among the outpatients and inpatients of a tertiary health institution was carried out using co...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | New Microbes and New Infections |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297522000713 |
| _version_ | 1811221734236356608 |
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| author | A.A. Adegoke W.E. Ikott A.I. Okoh |
| author_facet | A.A. Adegoke W.E. Ikott A.I. Okoh |
| author_sort | A.A. Adegoke |
| collection | DOAJ |
| description | The World Health Organization in 2017 listed carbapenem resistant Enterobacteriaceae (CRE) with critical priority for research. A research to assess carbapenem resistant Escherichia coli (CREc) in coliuria among the outpatients and inpatients of a tertiary health institution was carried out using conventional methods, polymerase chain reaction, Sanger sequencing, and bioinformatics. There were 39 positive coliuria cases from the urine samples collected from a total of 126 patients with various genitourinary diseases. The E. coli enumeration (log10 CFU/mL) revealed that 82.1% (n = 32) of the samples showed significant coliuria, 12.8% (n = 5) showed non-significant coliuria while 5.1% (n = 2) showed indeterminate coliuria even when repeated. Significantly higher numbers (p > 0.05) of the sampled inpatients yielded positive coliuria (57.9%) than the outpatients. Though there were significantly more (P > 0.05) urology female patients (n = 77) than male (n = 49), coliuria was more prevalent in sampled male patients (34.9%) than female (28.6%). Highest prevalence of coliuria was observed among the age range (18–30) years. Selected CREc that was sequenced and the sequences submitted to GenBank of National Center for Biotechnological Information (NCBI) were Escherichia coli AYO-WINI111 and Escherichia coli AYO-WINI112 with accession number MT735391 and MT735392, respectively. High resistance was observed against ertapenem (53%), imipenem (62%), meropenem (48%), and doripenem (47%), while 7%–22% of the isolates showed phenotypic intermediate carbapenem resistance. Critically dangerous CREc are harboured by large number urology patients in the study area, depicting the need for more attention in the management of the condition, as CREc are close to achieving totally antibiotic resistance. |
| first_indexed | 2024-04-12T08:04:28Z |
| format | Article |
| id | doaj.art-81081916faf44b888f99e019ea0aed5c |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-04-12T08:04:28Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj.art-81081916faf44b888f99e019ea0aed5c2022-12-22T03:41:12ZengElsevierNew Microbes and New Infections2052-29752022-07-0148101019Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patientsA.A. Adegoke0W.E. Ikott1A.I. Okoh2Department of Microbiology, University of Uyo, Uyo, Nigeria; Adjunct Researcher, Faculty of Health Sciences, Durban University of Technology (DUT), Durban, South Africa; TETFUND Centre of Excellence for Artificial Intelligence, University of Uyo, Uyo, Nigeria; Institute for Water and Wastewater Technology (IWWT), Durban University of Technology (DUT), Durban, South Africa; Corresponding author. A.A. Adegoke, Department of Microbiology, University of Uyo, Uyo, NigeriaDepartment of Microbiology, University of Uyo, Uyo, NigeriaDepartment of Environmental Health Sciences, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates; SAMRC Microbial Water Quality Monitoring Centre, University of for Hare, Alice, South AfricaThe World Health Organization in 2017 listed carbapenem resistant Enterobacteriaceae (CRE) with critical priority for research. A research to assess carbapenem resistant Escherichia coli (CREc) in coliuria among the outpatients and inpatients of a tertiary health institution was carried out using conventional methods, polymerase chain reaction, Sanger sequencing, and bioinformatics. There were 39 positive coliuria cases from the urine samples collected from a total of 126 patients with various genitourinary diseases. The E. coli enumeration (log10 CFU/mL) revealed that 82.1% (n = 32) of the samples showed significant coliuria, 12.8% (n = 5) showed non-significant coliuria while 5.1% (n = 2) showed indeterminate coliuria even when repeated. Significantly higher numbers (p > 0.05) of the sampled inpatients yielded positive coliuria (57.9%) than the outpatients. Though there were significantly more (P > 0.05) urology female patients (n = 77) than male (n = 49), coliuria was more prevalent in sampled male patients (34.9%) than female (28.6%). Highest prevalence of coliuria was observed among the age range (18–30) years. Selected CREc that was sequenced and the sequences submitted to GenBank of National Center for Biotechnological Information (NCBI) were Escherichia coli AYO-WINI111 and Escherichia coli AYO-WINI112 with accession number MT735391 and MT735392, respectively. High resistance was observed against ertapenem (53%), imipenem (62%), meropenem (48%), and doripenem (47%), while 7%–22% of the isolates showed phenotypic intermediate carbapenem resistance. Critically dangerous CREc are harboured by large number urology patients in the study area, depicting the need for more attention in the management of the condition, as CREc are close to achieving totally antibiotic resistance.http://www.sciencedirect.com/science/article/pii/S2052297522000713Carbapenem-resistant enterobacteriaceaecoliuriaimipenemmeropenem resistance |
| spellingShingle | A.A. Adegoke W.E. Ikott A.I. Okoh Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients New Microbes and New Infections Carbapenem-resistant enterobacteriaceae coliuria imipenem meropenem resistance |
| title | Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| title_full | Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| title_fullStr | Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| title_full_unstemmed | Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| title_short | Carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| title_sort | carbapenem resistance associated with coliuria among outpatient and hospitalised urology patients |
| topic | Carbapenem-resistant enterobacteriaceae coliuria imipenem meropenem resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2052297522000713 |
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