Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy
Chitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407) as gelling agents for oral drug delivery. The performance interaction with mucin of chitosan-composed gel (F1) and PVP-composed gel (F2) was compared using attenuated t...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/7/1142 |
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| author | Prangtip Uthaiwat Aroonsri Priprem Ploenthip Puthongking Jureerut Daduang Chatchanok Nukulkit Sirinart Chio-Srichan Patcharee Boonsiri Suthasinee Thapphasaraphong |
| author_facet | Prangtip Uthaiwat Aroonsri Priprem Ploenthip Puthongking Jureerut Daduang Chatchanok Nukulkit Sirinart Chio-Srichan Patcharee Boonsiri Suthasinee Thapphasaraphong |
| author_sort | Prangtip Uthaiwat |
| collection | DOAJ |
| description | Chitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407) as gelling agents for oral drug delivery. The performance interaction with mucin of chitosan-composed gel (F1) and PVP-composed gel (F2) was compared using attenuated total reflectance–Fourier-transform infrared (ATR-FTIR) spectroscopy at controlled temperatures of 25 and 37 °C for 1 and 5 min. F1 containing niosome-entrapped melatonin or its derivatives was investigated for mucoadhesive interaction on mucosa by ATR-FTIR spectroscopy under the same conditions. The results showed that F1-treated mucin gave a significantly lower amide I/amide II ratio than untreated mucin and F2-treated mucin did within 1 min, suggesting improved rapid affinity between mucin and chitosan. The spectra of mucosa treated with F1 incorporating niosomes of melatonin or its derivatives showed peak shifts at C=O (amide I), N-H (amide II), and carbohydrate regions and an associated decrease in the amide I/amide II ratio and increase in the carbohydrate/amide II ratio. These results indicated electrostatic interaction and hydrogen bonding between chitosan and mucin on the mucosa. In conclusion, the molecular interaction between gels and mucin/mucosa detected at amide I and amide II of proteins and the carbohydrate region could lead to an improved mucoadhesive property of the gel on the mucosa. |
| first_indexed | 2024-03-10T12:38:43Z |
| format | Article |
| id | doaj.art-810cfcb468914fe7a769bcedc2d9a82e |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T12:38:43Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-810cfcb468914fe7a769bcedc2d9a82e2023-11-21T14:04:16ZengMDPI AGPolymers2073-43602021-04-01137114210.3390/polym13071142Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR SpectroscopyPrangtip Uthaiwat0Aroonsri Priprem1Ploenthip Puthongking2Jureerut Daduang3Chatchanok Nukulkit4Sirinart Chio-Srichan5Patcharee Boonsiri6Suthasinee Thapphasaraphong7Graduate School, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Pharmacy, Mahasarakham University, Maha Sarakham 44150, ThailandMelatonin Research Group, Khon Kaen University, Khon Kaen 40002, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Thai Traditional Medicine, Rajamangala University of Technology Isan Sakon Nakhon Campus, Sakon Nakhon 47160, ThailandSynchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, ThailandDepartment of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandMelatonin Research Group, Khon Kaen University, Khon Kaen 40002, ThailandChitosan or polyvinyl pyrrolidone (PVP) were used in combination with hydroxypropyl methylcellulose (HPMC) and poloxamer 407 (P407) as gelling agents for oral drug delivery. The performance interaction with mucin of chitosan-composed gel (F1) and PVP-composed gel (F2) was compared using attenuated total reflectance–Fourier-transform infrared (ATR-FTIR) spectroscopy at controlled temperatures of 25 and 37 °C for 1 and 5 min. F1 containing niosome-entrapped melatonin or its derivatives was investigated for mucoadhesive interaction on mucosa by ATR-FTIR spectroscopy under the same conditions. The results showed that F1-treated mucin gave a significantly lower amide I/amide II ratio than untreated mucin and F2-treated mucin did within 1 min, suggesting improved rapid affinity between mucin and chitosan. The spectra of mucosa treated with F1 incorporating niosomes of melatonin or its derivatives showed peak shifts at C=O (amide I), N-H (amide II), and carbohydrate regions and an associated decrease in the amide I/amide II ratio and increase in the carbohydrate/amide II ratio. These results indicated electrostatic interaction and hydrogen bonding between chitosan and mucin on the mucosa. In conclusion, the molecular interaction between gels and mucin/mucosa detected at amide I and amide II of proteins and the carbohydrate region could lead to an improved mucoadhesive property of the gel on the mucosa.https://www.mdpi.com/2073-4360/13/7/1142gel base effectmucosaATR-FTIR spectroscopymelatonin niosome gel |
| spellingShingle | Prangtip Uthaiwat Aroonsri Priprem Ploenthip Puthongking Jureerut Daduang Chatchanok Nukulkit Sirinart Chio-Srichan Patcharee Boonsiri Suthasinee Thapphasaraphong Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy Polymers gel base effect mucosa ATR-FTIR spectroscopy melatonin niosome gel |
| title | Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy |
| title_full | Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy |
| title_fullStr | Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy |
| title_full_unstemmed | Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy |
| title_short | Characteristic Evaluation of Gel Formulation Containing Niosomes of Melatonin or Its Derivative and Mucoadhesive Properties Using ATR-FTIR Spectroscopy |
| title_sort | characteristic evaluation of gel formulation containing niosomes of melatonin or its derivative and mucoadhesive properties using atr ftir spectroscopy |
| topic | gel base effect mucosa ATR-FTIR spectroscopy melatonin niosome gel |
| url | https://www.mdpi.com/2073-4360/13/7/1142 |
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