Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis)
Abstract The Heilongjiang brown frog (Rana amurensis) is widely used in traditional Chinese medicine. In particular, the oviduct and skin have been developed into various health products. However, limited numbers of complete genomes of amphibian species have been reported, excluding the Heilongjiang...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24631-6 |
| _version_ | 1797985557901475840 |
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| author | Wanyu Li Yue Lan Lei Wang Lewei He Ruixiang Tang Megan Price Bisong Yue Zhenxin Fan |
| author_facet | Wanyu Li Yue Lan Lei Wang Lewei He Ruixiang Tang Megan Price Bisong Yue Zhenxin Fan |
| author_sort | Wanyu Li |
| collection | DOAJ |
| description | Abstract The Heilongjiang brown frog (Rana amurensis) is widely used in traditional Chinese medicine. In particular, the oviduct and skin have been developed into various health products. However, limited numbers of complete genomes of amphibian species have been reported, excluding the Heilongjiang brown frog. Here, the transcriptomes of 45 samples from the liver, spleen, heart, ovaries, thigh muscles, skin, oviduct, stomach and intestine of five Heilongjiang brown frog were reassembled and analyzed. A total of 1,085,532 unigenes with an average length of 676.6 bp and N50 of 722 bp were obtained. Comparative transcriptomics of different tissues detected tissue-specific expression. There were 3248 differentially expressed genes (DEGs) in the ovary, and the number of unique DEGs between the ovary and spleen was the largest. The results of DEGs enrichment showed there were many pathways and items related to protein synthesis and metabolism in the oviduct. The DEGs of the skin were enriched with many bacterial defense items, indicating that there were a large number of antimicrobial peptides in the skin. Thus, these were suitable as biological sources for the development and extraction of antimicrobial peptides. Through the assembly of transcriptome sequencing data and functional annotation of the Heilongjiang brown frog genome, this study provides reference materials for further exploring and utilizing functional gene resources of frogs and lays a foundation for medical research and the development of new products. |
| first_indexed | 2024-04-11T07:19:57Z |
| format | Article |
| id | doaj.art-810e1d0b42f840e08bad2edaab72ee30 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:19:57Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-810e1d0b42f840e08bad2edaab72ee302022-12-22T04:37:48ZengNature PortfolioScientific Reports2045-23222022-12-011211910.1038/s41598-022-24631-6Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis)Wanyu Li0Yue Lan1Lei Wang2Lewei He3Ruixiang Tang4Megan Price5Bisong Yue6Zhenxin Fan7Key Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversitySichuan Key Laboratory of Conservation Biology On Endangered Wildlife, College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityKey Laboratory of Bioresources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan UniversityAbstract The Heilongjiang brown frog (Rana amurensis) is widely used in traditional Chinese medicine. In particular, the oviduct and skin have been developed into various health products. However, limited numbers of complete genomes of amphibian species have been reported, excluding the Heilongjiang brown frog. Here, the transcriptomes of 45 samples from the liver, spleen, heart, ovaries, thigh muscles, skin, oviduct, stomach and intestine of five Heilongjiang brown frog were reassembled and analyzed. A total of 1,085,532 unigenes with an average length of 676.6 bp and N50 of 722 bp were obtained. Comparative transcriptomics of different tissues detected tissue-specific expression. There were 3248 differentially expressed genes (DEGs) in the ovary, and the number of unique DEGs between the ovary and spleen was the largest. The results of DEGs enrichment showed there were many pathways and items related to protein synthesis and metabolism in the oviduct. The DEGs of the skin were enriched with many bacterial defense items, indicating that there were a large number of antimicrobial peptides in the skin. Thus, these were suitable as biological sources for the development and extraction of antimicrobial peptides. Through the assembly of transcriptome sequencing data and functional annotation of the Heilongjiang brown frog genome, this study provides reference materials for further exploring and utilizing functional gene resources of frogs and lays a foundation for medical research and the development of new products.https://doi.org/10.1038/s41598-022-24631-6 |
| spellingShingle | Wanyu Li Yue Lan Lei Wang Lewei He Ruixiang Tang Megan Price Bisong Yue Zhenxin Fan Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) Scientific Reports |
| title | Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) |
| title_full | Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) |
| title_fullStr | Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) |
| title_full_unstemmed | Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) |
| title_short | Comparative transcriptomes of nine tissues for the Heilongjiang brown frog (Rana amurensis) |
| title_sort | comparative transcriptomes of nine tissues for the heilongjiang brown frog rana amurensis |
| url | https://doi.org/10.1038/s41598-022-24631-6 |
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