Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media

Abstract Optical imaging in scattering media is important to many fields but remains challenging. Recent methods have focused on imaging through thin scattering layers or thicker scattering media with prior knowledge of the sample, but this still limits practical applications. Here, we report an ima...

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Main Authors: Yaoyao Shi, Wei Sheng, Yangyang Fu, Youwen Liu
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-43674-5
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author Yaoyao Shi
Wei Sheng
Yangyang Fu
Youwen Liu
author_facet Yaoyao Shi
Wei Sheng
Yangyang Fu
Youwen Liu
author_sort Yaoyao Shi
collection DOAJ
description Abstract Optical imaging in scattering media is important to many fields but remains challenging. Recent methods have focused on imaging through thin scattering layers or thicker scattering media with prior knowledge of the sample, but this still limits practical applications. Here, we report an imaging method named ‘speckle kinetography’ that enables high-resolution imaging in unknown scattering media with thicknesses up to about 6 transport mean free paths. Speckle kinetography non-invasively records a series of incoherent speckle images accompanied by object motion and the inherently retained object information is extracted through an overlapping speckle correlation algorithm to construct the object’s autocorrelation for imaging. Under single-colour light-emitting diode, white light, and fluorescence illumination, we experimentally demonstrate 1 μm resolution imaging and tracking of objects moving in scattering samples, while reducing the requirements for prior knowledge. We anticipate this method will enable imaging in currently inaccessible scenarios.
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spelling doaj.art-810f54fbdb524768845b42b62b03e40e2023-11-26T13:45:33ZengNature PortfolioNature Communications2041-17232023-11-011411810.1038/s41467-023-43674-5Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering mediaYaoyao Shi0Wei Sheng1Yangyang Fu2Youwen Liu3College of Physics, Nanjing University of Aeronautics and AstronauticsCollege of Physics, Nanjing University of Aeronautics and AstronauticsCollege of Physics, Nanjing University of Aeronautics and AstronauticsCollege of Physics, Nanjing University of Aeronautics and AstronauticsAbstract Optical imaging in scattering media is important to many fields but remains challenging. Recent methods have focused on imaging through thin scattering layers or thicker scattering media with prior knowledge of the sample, but this still limits practical applications. Here, we report an imaging method named ‘speckle kinetography’ that enables high-resolution imaging in unknown scattering media with thicknesses up to about 6 transport mean free paths. Speckle kinetography non-invasively records a series of incoherent speckle images accompanied by object motion and the inherently retained object information is extracted through an overlapping speckle correlation algorithm to construct the object’s autocorrelation for imaging. Under single-colour light-emitting diode, white light, and fluorescence illumination, we experimentally demonstrate 1 μm resolution imaging and tracking of objects moving in scattering samples, while reducing the requirements for prior knowledge. We anticipate this method will enable imaging in currently inaccessible scenarios.https://doi.org/10.1038/s41467-023-43674-5
spellingShingle Yaoyao Shi
Wei Sheng
Yangyang Fu
Youwen Liu
Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
Nature Communications
title Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
title_full Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
title_fullStr Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
title_full_unstemmed Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
title_short Overlapping speckle correlation algorithm for high-resolution imaging and tracking of objects in unknown scattering media
title_sort overlapping speckle correlation algorithm for high resolution imaging and tracking of objects in unknown scattering media
url https://doi.org/10.1038/s41467-023-43674-5
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AT youwenliu overlappingspecklecorrelationalgorithmforhighresolutionimagingandtrackingofobjectsinunknownscatteringmedia