Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis
Drug-resistant tuberculosis (TB) is one of the most lethal diseases, and it is imperative to exploit an advanced drug formulation for its effective treatment. This work aims to develop a mannose receptor-targeted bioadhesive chitosan nanoparticles for effective drug-resistant tuberculosis treatment....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016420302437 |
| _version_ | 1818449874067128320 |
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| author | Datta Maroti Pawde Matte Kasi Viswanadh Abhishesh Kumar Mehata Roshan Sonkar Narendra Suruchi Poddar Ankita Sanjay Burande Abhishek Jha Kiran Yellappa Vajanthri Sanjeev Kumar Mahto V.N. Azger Dustakeer Madaswamy S. Muthu |
| author_facet | Datta Maroti Pawde Matte Kasi Viswanadh Abhishesh Kumar Mehata Roshan Sonkar Narendra Suruchi Poddar Ankita Sanjay Burande Abhishek Jha Kiran Yellappa Vajanthri Sanjeev Kumar Mahto V.N. Azger Dustakeer Madaswamy S. Muthu |
| author_sort | Datta Maroti Pawde |
| collection | DOAJ |
| description | Drug-resistant tuberculosis (TB) is one of the most lethal diseases, and it is imperative to exploit an advanced drug formulation for its effective treatment. This work aims to develop a mannose receptor-targeted bioadhesive chitosan nanoparticles for effective drug-resistant tuberculosis treatment. The clofazimine loaded chitosan nanoparticles were formulated; their size, charge, polydispersity (PDI), surface morphology, entrapment efficiency (EE) and in-vitro release pattern were established. Also, cellular uptake study on C2C12 cell lines and anti-mycobacterial activity against H37Rv (a standard strain of Mycobacterium tuberculosis) were evaluated. The particle sizes of formulated chitosan nanoparticles were in the range of 132–184 nm and EE was also found to be between 73 and 95%. The functionalization of bioadhesive chitosan nanoparticles with mannose was confirmed by infrared spectroscopy (FTIR). The uptake studies on the C2C12 cell lines showed that mannosylated nanoparticles were more efficiently internalized when compared to non-targeted nanoparticles. Further, luciferase reporter phage (LRP) assay against H37Rv strain showed that clofazimine nanoparticles were found to be 49.5 times superior in terms of inhibition and anti-mycobacterial activity than free clofazimine. This excellent activity might be attributed to enhanced drug delivery with a promising bioadhesion property of chitosan-based nanoparticles. |
| first_indexed | 2024-12-14T20:42:20Z |
| format | Article |
| id | doaj.art-8111eb418b5b40a4a34a3d59680330a6 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-14T20:42:20Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-8111eb418b5b40a4a34a3d59680330a62022-12-21T22:48:13ZengElsevierSaudi Pharmaceutical Journal1319-01642020-12-01281216161625Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosisDatta Maroti Pawde0Matte Kasi Viswanadh1Abhishesh Kumar Mehata2Roshan Sonkar3 Narendra4Suruchi Poddar5Ankita Sanjay Burande6Abhishek Jha7Kiran Yellappa Vajanthri8Sanjeev Kumar Mahto9V.N. Azger Dustakeer10Madaswamy S. Muthu11Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaSchool of Biomedical Engineering, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, IndiaSchool of Biomedical Engineering, Indian Institute of Technology (BHU), Varanasi 221005, IndiaSchool of Biomedical Engineering, Indian Institute of Technology (BHU), Varanasi 221005, IndiaDepartment of Bacteriology, National Institute for Research in Tuberculosis, Chetpet, Chennai 600031, IndiaDepartment of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India; Corresponding author.Drug-resistant tuberculosis (TB) is one of the most lethal diseases, and it is imperative to exploit an advanced drug formulation for its effective treatment. This work aims to develop a mannose receptor-targeted bioadhesive chitosan nanoparticles for effective drug-resistant tuberculosis treatment. The clofazimine loaded chitosan nanoparticles were formulated; their size, charge, polydispersity (PDI), surface morphology, entrapment efficiency (EE) and in-vitro release pattern were established. Also, cellular uptake study on C2C12 cell lines and anti-mycobacterial activity against H37Rv (a standard strain of Mycobacterium tuberculosis) were evaluated. The particle sizes of formulated chitosan nanoparticles were in the range of 132–184 nm and EE was also found to be between 73 and 95%. The functionalization of bioadhesive chitosan nanoparticles with mannose was confirmed by infrared spectroscopy (FTIR). The uptake studies on the C2C12 cell lines showed that mannosylated nanoparticles were more efficiently internalized when compared to non-targeted nanoparticles. Further, luciferase reporter phage (LRP) assay against H37Rv strain showed that clofazimine nanoparticles were found to be 49.5 times superior in terms of inhibition and anti-mycobacterial activity than free clofazimine. This excellent activity might be attributed to enhanced drug delivery with a promising bioadhesion property of chitosan-based nanoparticles.http://www.sciencedirect.com/science/article/pii/S1319016420302437ChitosanClofazimineMannose receptorNanoparticlesTuberculosis |
| spellingShingle | Datta Maroti Pawde Matte Kasi Viswanadh Abhishesh Kumar Mehata Roshan Sonkar Narendra Suruchi Poddar Ankita Sanjay Burande Abhishek Jha Kiran Yellappa Vajanthri Sanjeev Kumar Mahto V.N. Azger Dustakeer Madaswamy S. Muthu Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis Saudi Pharmaceutical Journal Chitosan Clofazimine Mannose receptor Nanoparticles Tuberculosis |
| title | Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| title_full | Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| title_fullStr | Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| title_full_unstemmed | Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| title_short | Mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| title_sort | mannose receptor targeted bioadhesive chitosan nanoparticles of clofazimine for effective therapy of tuberculosis |
| topic | Chitosan Clofazimine Mannose receptor Nanoparticles Tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S1319016420302437 |
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