Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species.
Bacterial biofilms are among the most abundant multicellular structures on Earth and play essential roles in a wide range of ecological, medical, and industrial processes. However, general principles that govern the emergence of biofilm architecture across different species remain unknown. Here, we...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-10-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3001846 |
| _version_ | 1817976048510304256 |
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| author | Hannah Jeckel Francisco Díaz-Pascual Dominic J Skinner Boya Song Eva Jiménez-Siebert Kerstin Strenger Eric Jelli Sanika Vaidya Jörn Dunkel Knut Drescher |
| author_facet | Hannah Jeckel Francisco Díaz-Pascual Dominic J Skinner Boya Song Eva Jiménez-Siebert Kerstin Strenger Eric Jelli Sanika Vaidya Jörn Dunkel Knut Drescher |
| author_sort | Hannah Jeckel |
| collection | DOAJ |
| description | Bacterial biofilms are among the most abundant multicellular structures on Earth and play essential roles in a wide range of ecological, medical, and industrial processes. However, general principles that govern the emergence of biofilm architecture across different species remain unknown. Here, we combine experiments, simulations, and statistical analysis to identify shared biophysical mechanisms that determine early biofilm architecture development at the single-cell level, for the species Vibrio cholerae, Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa grown as microcolonies in flow chambers. Our data-driven analysis reveals that despite the many molecular differences between these species, the biofilm architecture differences can be described by only 2 control parameters: cellular aspect ratio and cell density. Further experiments using single-species mutants for which the cell aspect ratio and the cell density are systematically varied, and mechanistic simulations show that tuning these 2 control parameters reproduces biofilm architectures of different species. Altogether, our results show that biofilm microcolony architecture is determined by mechanical cell-cell interactions, which are conserved across different species. |
| first_indexed | 2024-04-13T21:58:20Z |
| format | Article |
| id | doaj.art-811345c31cd64032806077176d0f2a31 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-04-13T21:58:20Z |
| publishDate | 2022-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-811345c31cd64032806077176d0f2a312022-12-22T02:28:11ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852022-10-012010e300184610.1371/journal.pbio.3001846Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species.Hannah JeckelFrancisco Díaz-PascualDominic J SkinnerBoya SongEva Jiménez-SiebertKerstin StrengerEric JelliSanika VaidyaJörn DunkelKnut DrescherBacterial biofilms are among the most abundant multicellular structures on Earth and play essential roles in a wide range of ecological, medical, and industrial processes. However, general principles that govern the emergence of biofilm architecture across different species remain unknown. Here, we combine experiments, simulations, and statistical analysis to identify shared biophysical mechanisms that determine early biofilm architecture development at the single-cell level, for the species Vibrio cholerae, Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa grown as microcolonies in flow chambers. Our data-driven analysis reveals that despite the many molecular differences between these species, the biofilm architecture differences can be described by only 2 control parameters: cellular aspect ratio and cell density. Further experiments using single-species mutants for which the cell aspect ratio and the cell density are systematically varied, and mechanistic simulations show that tuning these 2 control parameters reproduces biofilm architectures of different species. Altogether, our results show that biofilm microcolony architecture is determined by mechanical cell-cell interactions, which are conserved across different species.https://doi.org/10.1371/journal.pbio.3001846 |
| spellingShingle | Hannah Jeckel Francisco Díaz-Pascual Dominic J Skinner Boya Song Eva Jiménez-Siebert Kerstin Strenger Eric Jelli Sanika Vaidya Jörn Dunkel Knut Drescher Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. PLoS Biology |
| title | Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. |
| title_full | Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. |
| title_fullStr | Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. |
| title_full_unstemmed | Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. |
| title_short | Shared biophysical mechanisms determine early biofilm architecture development across different bacterial species. |
| title_sort | shared biophysical mechanisms determine early biofilm architecture development across different bacterial species |
| url | https://doi.org/10.1371/journal.pbio.3001846 |
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