Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology
Discarded eggshells and wet soybean dregs are rich sources of calcium and nitrogen. This study aimed to establish a novel optimized method for the utilization of agricultural wastes. Eggshells and soybean dregs were the primary raw materials used in this study. Bacillus subtilis, Lactobacillus plant...
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The editorial department of Science and Technology of Food Industry
2023-05-01
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Series: | Shipin gongye ke-ji |
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Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070321 |
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author | Shaoshu CHEN Liting DONG Fengteng XIA Yujie LIU Can LUO Yicheng LI Zheng WANG |
author_facet | Shaoshu CHEN Liting DONG Fengteng XIA Yujie LIU Can LUO Yicheng LI Zheng WANG |
author_sort | Shaoshu CHEN |
collection | DOAJ |
description | Discarded eggshells and wet soybean dregs are rich sources of calcium and nitrogen. This study aimed to establish a novel optimized method for the utilization of agricultural wastes. Eggshells and soybean dregs were the primary raw materials used in this study. Bacillus subtilis, Lactobacillus plantarum, and Enterococcus faecalis were the probiotic strains selected for mixed fermentation. Harman’s single-factor test based on the assessment of calcium lactate content in the fermented forage was used to screen the following process parameters: Ratio of soybean dregs to eggshells, solid to water ratio, addition of glucose, inoculation amount, ratio of probiotic strains, fermentation temperature and duration. Optimum fermentation conditions were determined using response surface methodology. The results revealed that the conditions required for optimum fermentation were as follows: Soybean dregs to eggshells ratio of 90:10, solid to water ratio of 1:2.5, addition of 15.6% glucose, inoculation amount of 14.7%, Bacillus subtilis, Lactobacillus plantarum and Enterococcus faecalis in the ratio of 1:1:1 (v/v), and fermentation duration of 6 days at 37 °C. The amount of calcium lactate in the fermented forage was 13.58%. Free amino acid and calcium lactate concentrations in the fermented forage were significantly higher (P<0.05) in comparison to their concentrations in the raw materials. A significant decrease in pH and concentrations of crude fat and soluble protein in the fermented forage was also observed (P<0.05). In conclusion, this study established a novel optimized method for the production of high calcium feed from eggshells and soybean dregs using probiotic fermentation. |
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spelling | doaj.art-8114f7fdb82144c19dbb8a0b18a12d3f2023-08-24T06:01:46ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-05-0144920020610.13386/j.issn1002-0306.20220703212022070321-9Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface MethodologyShaoshu CHEN0Liting DONG1Fengteng XIA2Yujie LIU3Can LUO4Yicheng LI5Zheng WANG6College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaHunan Imagination Agricultural Science and Technology, Zhangjiajie 427000, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaDiscarded eggshells and wet soybean dregs are rich sources of calcium and nitrogen. This study aimed to establish a novel optimized method for the utilization of agricultural wastes. Eggshells and soybean dregs were the primary raw materials used in this study. Bacillus subtilis, Lactobacillus plantarum, and Enterococcus faecalis were the probiotic strains selected for mixed fermentation. Harman’s single-factor test based on the assessment of calcium lactate content in the fermented forage was used to screen the following process parameters: Ratio of soybean dregs to eggshells, solid to water ratio, addition of glucose, inoculation amount, ratio of probiotic strains, fermentation temperature and duration. Optimum fermentation conditions were determined using response surface methodology. The results revealed that the conditions required for optimum fermentation were as follows: Soybean dregs to eggshells ratio of 90:10, solid to water ratio of 1:2.5, addition of 15.6% glucose, inoculation amount of 14.7%, Bacillus subtilis, Lactobacillus plantarum and Enterococcus faecalis in the ratio of 1:1:1 (v/v), and fermentation duration of 6 days at 37 °C. The amount of calcium lactate in the fermented forage was 13.58%. Free amino acid and calcium lactate concentrations in the fermented forage were significantly higher (P<0.05) in comparison to their concentrations in the raw materials. A significant decrease in pH and concentrations of crude fat and soluble protein in the fermented forage was also observed (P<0.05). In conclusion, this study established a novel optimized method for the production of high calcium feed from eggshells and soybean dregs using probiotic fermentation.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070321soybean dregseggshellssolid fermentationcalcium lactatehigh calcium feed |
spellingShingle | Shaoshu CHEN Liting DONG Fengteng XIA Yujie LIU Can LUO Yicheng LI Zheng WANG Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology Shipin gongye ke-ji soybean dregs eggshells solid fermentation calcium lactate high calcium feed |
title | Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology |
title_full | Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology |
title_fullStr | Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology |
title_full_unstemmed | Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology |
title_short | Optimized the Fermentation Process of Eggshell and Soybean Dregs to Produce High Calcium Feed by Response Surface Methodology |
title_sort | optimized the fermentation process of eggshell and soybean dregs to produce high calcium feed by response surface methodology |
topic | soybean dregs eggshells solid fermentation calcium lactate high calcium feed |
url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070321 |
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