Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition
The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Myt...
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MDPI AG
2017-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/15/9/294 |
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| author | Fuyan Liu Xiaofeng Zhang Yuqiu Li Qixin Chen Fei Liu Xiqiang Zhu Li Mei Xinlei Song Xia Liu Zhigang Song Jinhua Zhang Wen Zhang Peixue Ling Fengshan Wang |
| author_facet | Fuyan Liu Xiaofeng Zhang Yuqiu Li Qixin Chen Fei Liu Xiqiang Zhu Li Mei Xinlei Song Xia Liu Zhigang Song Jinhua Zhang Wen Zhang Peixue Ling Fengshan Wang |
| author_sort | Fuyan Liu |
| collection | DOAJ |
| description | The hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction. |
| first_indexed | 2024-04-11T18:44:19Z |
| format | Article |
| id | doaj.art-811c98650da344d3b9771a699dbb689b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:44:19Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-811c98650da344d3b9771a699dbb689b2022-12-22T04:08:53ZengMDPI AGMarine Drugs1660-33972017-09-0115929410.3390/md15090294md15090294Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway InhibitionFuyan Liu0Xiaofeng Zhang1Yuqiu Li2Qixin Chen3Fei Liu4Xiqiang Zhu5Li Mei6Xinlei Song7Xia Liu8Zhigang Song9Jinhua Zhang10Wen Zhang11Peixue Ling12Fengshan Wang13Shandong Academy of Pharmaceutical Science, Jinan 250101, ChinaSchool of Life Sciences, Lanzhou University, Lanzhou 730000, ChinaShandong University of Traditional Chinese Medicine, Jinan 250355, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan 250012, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaSchool of Pharmacy, Second Military Medical University, Shanghai 200433, ChinaShandong Academy of Pharmaceutical Science, Jinan 250101, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan 250012, ChinaThe hard-shelled mussel (Mytilus coruscus) has been used as Chinese traditional medicine for thousands of years; however, to date the ingredients responsible for the various beneficial health outcomes attributed to Mytilus coruscus are still unclear. An α-d-Glucan, called MP-A, was isolated from Mytilus coruscus, and observed to exert anti-inflammatory activity in THP-1 human macrophage cells. Specifically, we showed that MP-A treatment inhibited the production of inflammatory markers, including TNF-α, NO, and PGE2, inducible NOS (iNOS), and cyclooxygenase-2 (COX-2), in LPS-activated THP-1 cells. It was also shown to enhance phagocytosis in the analyzed cells, but to severely inhibit the phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear translocation of NF-κB P65. Finally, MP-A was found to exhibit a high binding affinity for the cell surface receptor TLR4, but a low affinity for TLR2 and dectin-1, via surface plasmon resonance (SPR) analysis. The study indicates that MP-A suppresses LPS-induced TNF-α, NO and PEG2 production via TLR4/NF-κB/MAPK pathway inhibition, and suggests that MP-A may be a promising therapeutic candidate for diseases associated with TNF-α, NO, and/or PEG2 overproduction.https://www.mdpi.com/1660-3397/15/9/294THP-1 macrophagesanti-inflammatoryTLR4NF-κBMAPKSPR analysis |
| spellingShingle | Fuyan Liu Xiaofeng Zhang Yuqiu Li Qixin Chen Fei Liu Xiqiang Zhu Li Mei Xinlei Song Xia Liu Zhigang Song Jinhua Zhang Wen Zhang Peixue Ling Fengshan Wang Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition Marine Drugs THP-1 macrophages anti-inflammatory TLR4 NF-κB MAPK SPR analysis |
| title | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
| title_full | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
| title_fullStr | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
| title_full_unstemmed | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
| title_short | Anti-Inflammatory Effects of a Mytilus coruscus α-d-Glucan (MP-A) in Activated Macrophage Cells via TLR4/NF-κB/MAPK Pathway Inhibition |
| title_sort | anti inflammatory effects of a mytilus coruscus α d glucan mp a in activated macrophage cells via tlr4 nf κb mapk pathway inhibition |
| topic | THP-1 macrophages anti-inflammatory TLR4 NF-κB MAPK SPR analysis |
| url | https://www.mdpi.com/1660-3397/15/9/294 |
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