The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH
<p>Abstract</p> <p>Background</p> <p>The tyramine producer <it>Enterococcus durans </it>IPLA655 contains all the necessary genes for tyramine biosynthesis, grouped in the TDC cluster. This cluster includes <it>tyrS</it>, an aminoacyl-tRNA synthet...
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| Format: | Article |
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BMC
2012-02-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/23 |
| _version_ | 1818443613046046720 |
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| author | Linares Daniel M Fernández Maria Del-Río Beatriz Ladero Victor Martin Maria Alvarez Miguel A |
| author_facet | Linares Daniel M Fernández Maria Del-Río Beatriz Ladero Victor Martin Maria Alvarez Miguel A |
| author_sort | Linares Daniel M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The tyramine producer <it>Enterococcus durans </it>IPLA655 contains all the necessary genes for tyramine biosynthesis, grouped in the TDC cluster. This cluster includes <it>tyrS</it>, an aminoacyl-tRNA synthetase like gene.</p> <p>Results</p> <p>This work shows that <it>tyrS </it>was maximally transcribed in absence of tyrosine at acidic pH, showing a greater than 10-fold induction in mRNA levels over levels occurring in presence of tyrosine. Mapping of the <it>tyrS </it>transcriptional start site revealed an unusually long untranslated leader region of 322 bp, which displays the typical features of the T box transcriptional attenuation mechanism. The tyrosine concentration regulation of <it>tyrS </it>was found to be mediated by a transcription antitermination system, whereas the specific induction at acidic pH was regulated at transcription initiation level.</p> <p>Conclusions</p> <p>The expression of the <it>tyrS </it>gene present in the TDC cluster of <it>E. durans </it>is transcriptionally regulated by tyrosine concentration and extracelular pH. The regulation is mediated by both an antitermination system and the promoter itself.</p> |
| first_indexed | 2024-12-14T19:02:49Z |
| format | Article |
| id | doaj.art-811e5f57648b4e03b5e9323eaa04bf24 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T19:02:49Z |
| publishDate | 2012-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-811e5f57648b4e03b5e9323eaa04bf242022-12-21T22:50:55ZengBMCBMC Microbiology1471-21802012-02-011212310.1186/1471-2180-12-23The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pHLinares Daniel MFernández MariaDel-Río BeatrizLadero VictorMartin MariaAlvarez Miguel A<p>Abstract</p> <p>Background</p> <p>The tyramine producer <it>Enterococcus durans </it>IPLA655 contains all the necessary genes for tyramine biosynthesis, grouped in the TDC cluster. This cluster includes <it>tyrS</it>, an aminoacyl-tRNA synthetase like gene.</p> <p>Results</p> <p>This work shows that <it>tyrS </it>was maximally transcribed in absence of tyrosine at acidic pH, showing a greater than 10-fold induction in mRNA levels over levels occurring in presence of tyrosine. Mapping of the <it>tyrS </it>transcriptional start site revealed an unusually long untranslated leader region of 322 bp, which displays the typical features of the T box transcriptional attenuation mechanism. The tyrosine concentration regulation of <it>tyrS </it>was found to be mediated by a transcription antitermination system, whereas the specific induction at acidic pH was regulated at transcription initiation level.</p> <p>Conclusions</p> <p>The expression of the <it>tyrS </it>gene present in the TDC cluster of <it>E. durans </it>is transcriptionally regulated by tyrosine concentration and extracelular pH. The regulation is mediated by both an antitermination system and the promoter itself.</p>http://www.biomedcentral.com/1471-2180/12/23 |
| spellingShingle | Linares Daniel M Fernández Maria Del-Río Beatriz Ladero Victor Martin Maria Alvarez Miguel A The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH BMC Microbiology |
| title | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH |
| title_full | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH |
| title_fullStr | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH |
| title_full_unstemmed | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH |
| title_short | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of <it>Enterococcus durans </it>is transcriptionally regulated by tyrosine concentration and extracellular pH |
| title_sort | tyrosyl trna synthetase like gene located in the tyramine biosynthesis cluster of it enterococcus durans it is transcriptionally regulated by tyrosine concentration and extracellular ph |
| url | http://www.biomedcentral.com/1471-2180/12/23 |
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