Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus
The stress response after jasmonic acid (JA) treatment was studied in cell suspension cultures of Catharanthus roseus. The effect of JA on the primary and secondary metabolism was based on changes in profiles of fatty acids (FA) and terpenoid indole alkaloids (TIA). According to multivariate data an...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/19/7/10242 |
| _version_ | 1811258977700282368 |
|---|---|
| author | Guitele Dalia Goldhaber-Pasillas Natali Rianika Mustafa Robert Verpoorte |
| author_facet | Guitele Dalia Goldhaber-Pasillas Natali Rianika Mustafa Robert Verpoorte |
| author_sort | Guitele Dalia Goldhaber-Pasillas |
| collection | DOAJ |
| description | The stress response after jasmonic acid (JA) treatment was studied in cell suspension cultures of Catharanthus roseus. The effect of JA on the primary and secondary metabolism was based on changes in profiles of fatty acids (FA) and terpenoid indole alkaloids (TIA). According to multivariate data analyses (MVDA), three major time events were observed and characterized according to the variations of specific FA and TIA: after 0–30 min of induction FA such as C18:1, C20:0, C22:0 and C24:0 were highly induced by JA; 90–360 min after treatment was characterized by variations of C14:0 and C15:0; and 1440 min after induction JA had the largest effect on both group of metabolites were C18:1, C18:2, C18:3, C16:0, C20:0, C22:0, C24:0, catharanthine, tabersonine-like 1, serpentine, tabersonine and ajmalicine-like had the most significant variations. These results unambiguously demonstrate the profound effect of JA particularly on the accumulation of its own precursor, C18:3 and the accumulation of TIA, which can be considered as late stress response events to JA since they occurred only after 1440 min. These observations show that the early events in the JA response do not involve the de novo biosynthesis of neither its own precursor nor TIA, but is due to an already present biochemical system. |
| first_indexed | 2024-04-12T18:22:38Z |
| format | Article |
| id | doaj.art-811f3beb3e7e47118089ce3c75b02075 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T18:22:38Z |
| publishDate | 2014-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-811f3beb3e7e47118089ce3c75b020752022-12-22T03:21:22ZengMDPI AGMolecules1420-30492014-07-01197102421026010.3390/molecules190710242molecules190710242Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseusGuitele Dalia Goldhaber-Pasillas0Natali Rianika Mustafa1Robert Verpoorte2Natural Products Laboratory, Institute of Biology Leiden, Leiden University, Sylvius Laboratoria, Sylviusweg 72, 2300 RA, Leiden, The NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden University, Sylvius Laboratoria, Sylviusweg 72, 2300 RA, Leiden, The NetherlandsNatural Products Laboratory, Institute of Biology Leiden, Leiden University, Sylvius Laboratoria, Sylviusweg 72, 2300 RA, Leiden, The NetherlandsThe stress response after jasmonic acid (JA) treatment was studied in cell suspension cultures of Catharanthus roseus. The effect of JA on the primary and secondary metabolism was based on changes in profiles of fatty acids (FA) and terpenoid indole alkaloids (TIA). According to multivariate data analyses (MVDA), three major time events were observed and characterized according to the variations of specific FA and TIA: after 0–30 min of induction FA such as C18:1, C20:0, C22:0 and C24:0 were highly induced by JA; 90–360 min after treatment was characterized by variations of C14:0 and C15:0; and 1440 min after induction JA had the largest effect on both group of metabolites were C18:1, C18:2, C18:3, C16:0, C20:0, C22:0, C24:0, catharanthine, tabersonine-like 1, serpentine, tabersonine and ajmalicine-like had the most significant variations. These results unambiguously demonstrate the profound effect of JA particularly on the accumulation of its own precursor, C18:3 and the accumulation of TIA, which can be considered as late stress response events to JA since they occurred only after 1440 min. These observations show that the early events in the JA response do not involve the de novo biosynthesis of neither its own precursor nor TIA, but is due to an already present biochemical system.http://www.mdpi.com/1420-3049/19/7/10242fatty acidsterpenoid indole alkaloidsMadagascar periwinklecell suspension culturesjasmonic acidmultivariate data analysis |
| spellingShingle | Guitele Dalia Goldhaber-Pasillas Natali Rianika Mustafa Robert Verpoorte Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus Molecules fatty acids terpenoid indole alkaloids Madagascar periwinkle cell suspension cultures jasmonic acid multivariate data analysis |
| title | Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus |
| title_full | Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus |
| title_fullStr | Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus |
| title_full_unstemmed | Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus |
| title_short | Jasmonic Acid Effect on the Fatty Acid and Terpenoid Indole Alkaloid Accumulation in Cell Suspension Cultures of Catharanthus roseus |
| title_sort | jasmonic acid effect on the fatty acid and terpenoid indole alkaloid accumulation in cell suspension cultures of catharanthus roseus |
| topic | fatty acids terpenoid indole alkaloids Madagascar periwinkle cell suspension cultures jasmonic acid multivariate data analysis |
| url | http://www.mdpi.com/1420-3049/19/7/10242 |
| work_keys_str_mv | AT guiteledaliagoldhaberpasillas jasmonicacideffectonthefattyacidandterpenoidindolealkaloidaccumulationincellsuspensionculturesofcatharanthusroseus AT natalirianikamustafa jasmonicacideffectonthefattyacidandterpenoidindolealkaloidaccumulationincellsuspensionculturesofcatharanthusroseus AT robertverpoorte jasmonicacideffectonthefattyacidandterpenoidindolealkaloidaccumulationincellsuspensionculturesofcatharanthusroseus |