Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran
Tylenchulus semipenetrans Cobb, 1913 is among some of the most economically important plant-parasitic nematodes in the world. The nematode has been identified as the causal agent of slow decline. Most studies estimate the yield losses, due to T. semipenetrans, to range from 10% to 30%, depending on...
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University of Belgrade - Faculty of Agriculture, Belgrade
2023-01-01
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| Series: | Journal of Agricultural Sciences (Belgrade) |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2023/1450-81092303301A.pdf |
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| author | Asadi Seyedeh Zohreh Jamali Salar Ghadamyari Mohammad Motaghitalab Vahid |
| author_facet | Asadi Seyedeh Zohreh Jamali Salar Ghadamyari Mohammad Motaghitalab Vahid |
| author_sort | Asadi Seyedeh Zohreh |
| collection | DOAJ |
| description | Tylenchulus semipenetrans Cobb, 1913 is among some of the most economically important plant-parasitic nematodes in the world. The nematode has been identified as the causal agent of slow decline. Most studies estimate the yield losses, due to T. semipenetrans, to range from 10% to 30%, depending on the level of infection, aggressiveness of the nematode population, soil characteristics, susceptibility of the rootstock, presence of other pathogens and grove management practices. In order to identify the citrus nematode in northern Iran, soil and root samples were collected from infected trees. The second-stage juveniles were isolated from the soils by the tray method. Eggs and females were extracted from roots by the centrifugal-flotation technique. Morphological observations and molecular evidence confirmed this population as T. semipenetrans. A phylogenetic tree of T. semipenetrans populations was reconstructed based on 28S rRNA gene sequences using RAxML. Morphologically, there is a slight difference between the studied population and the reported populations of T. semipenetrans from pomegranate and banana orchards in southern Iran. Phylogenetic analysis showed the close relationship of the T. semipenetrans population from northern Iran with other populations of this species. Based on molecular analysis, Tylenchulus was identified as a monophyletic group. The phylogenetic position and measurements of T. semipenetrans were provided. |
| first_indexed | 2024-03-09T00:49:40Z |
| format | Article |
| id | doaj.art-811f47273917420b835ed1f5b84ed2ec |
| institution | Directory Open Access Journal |
| issn | 1450-8109 2406-0968 |
| language | English |
| last_indexed | 2024-03-09T00:49:40Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade - Faculty of Agriculture, Belgrade |
| record_format | Article |
| series | Journal of Agricultural Sciences (Belgrade) |
| spelling | doaj.art-811f47273917420b835ed1f5b84ed2ec2023-12-11T17:15:43ZengUniversity of Belgrade - Faculty of Agriculture, BelgradeJournal of Agricultural Sciences (Belgrade)1450-81092406-09682023-01-0168330131410.2298/JAS2303301A1450-81092303301AMorphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern IranAsadi Seyedeh Zohreh0Jamali Salar1Ghadamyari Mohammad2Motaghitalab Vahid3University of Guilan, Faculty of Agricultural Sciences, Department of Plant Protection, Rasht, IranUniversity of Guilan, Faculty of Agricultural Sciences, Department of Plant Protection, Rasht, IranUniversity of Guilan, Faculty of Agricultural Sciences, Department of Plant Protection, Rasht, IranUniversity of Guilan, Faculty of Engineering Sciences, Department of Textile Engineering, Rasht, IranTylenchulus semipenetrans Cobb, 1913 is among some of the most economically important plant-parasitic nematodes in the world. The nematode has been identified as the causal agent of slow decline. Most studies estimate the yield losses, due to T. semipenetrans, to range from 10% to 30%, depending on the level of infection, aggressiveness of the nematode population, soil characteristics, susceptibility of the rootstock, presence of other pathogens and grove management practices. In order to identify the citrus nematode in northern Iran, soil and root samples were collected from infected trees. The second-stage juveniles were isolated from the soils by the tray method. Eggs and females were extracted from roots by the centrifugal-flotation technique. Morphological observations and molecular evidence confirmed this population as T. semipenetrans. A phylogenetic tree of T. semipenetrans populations was reconstructed based on 28S rRNA gene sequences using RAxML. Morphologically, there is a slight difference between the studied population and the reported populations of T. semipenetrans from pomegranate and banana orchards in southern Iran. Phylogenetic analysis showed the close relationship of the T. semipenetrans population from northern Iran with other populations of this species. Based on molecular analysis, Tylenchulus was identified as a monophyletic group. The phylogenetic position and measurements of T. semipenetrans were provided.https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2023/1450-81092303301A.pdfcitrus sinensis28s rrnairanphylogenyslow decline |
| spellingShingle | Asadi Seyedeh Zohreh Jamali Salar Ghadamyari Mohammad Motaghitalab Vahid Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran Journal of Agricultural Sciences (Belgrade) citrus sinensis 28s rrna iran phylogeny slow decline |
| title | Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran |
| title_full | Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran |
| title_fullStr | Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran |
| title_full_unstemmed | Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran |
| title_short | Morphology and molecular characterization of Tylenchulus semipenetrans from Citrus orchards in northern Iran |
| title_sort | morphology and molecular characterization of tylenchulus semipenetrans from citrus orchards in northern iran |
| topic | citrus sinensis 28s rrna iran phylogeny slow decline |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2023/1450-81092303301A.pdf |
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