<it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark
<p>Abstract</p> <p>Background</p> <p>Malaria is a major public health threat in Africa, and traditional medicine continues to play a key role in its control especially in rural areas. A bioassay-guided fractionation was carried out in order to evaluate the anti-malarial...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-06-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/10/1/167 |
| _version_ | 1818327481863634944 |
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| author | Tane Pierre Ngemenya Moses N Wabo Hippolyte K Kowa Théodora K Zofou Denis Titanji Vincent PK |
| author_facet | Tane Pierre Ngemenya Moses N Wabo Hippolyte K Kowa Théodora K Zofou Denis Titanji Vincent PK |
| author_sort | Tane Pierre |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Malaria is a major public health threat in Africa, and traditional medicine continues to play a key role in its control especially in rural areas. A bioassay-guided fractionation was carried out in order to evaluate the anti-malarial potential and the safety of the methanol extract of the <it>Hypericum lanceolatum </it>stem bark.</p> <p>Methods</p> <p>The anti-plasmodial activity was assayed by the lactate dehydrogenase method (pLDH) against the multidrug-resistant W2mef laboratory strain, and a field isolate (SHF4) of <it>Plasmodium falciparum</it>. Cytotoxicity tests were carried out using the LLC-MK2 monkey kidney epithelial cells.</p> <p>Results</p> <p>Five compounds were isolated from the most active and least cytotoxic ethylacetate sub-extract: betulinic acid (HLT1), 2,2',5,6'-tetrahydroxybenzophenone (HLT2), 5-hydroxy-3-methoxyxanthone (HLT3), 3-hydroxy-5-methoxyxanthone (HLT4) and HLT0 (yet to be identified). Three of the tested compounds presented significant anti-plasmodial activities (with 50% inhibitory concentration, IC<sub>50 </sub>< 5 μM), with 5-hydroxy-3-methoxyxanthone exerting the highest activity, followed by HLT0 and betulinic acid. All the compounds with significant anti-plasmodial activity were non-cytotoxic, except betulinic acid which showed a 50% cytotoxic concentration, CC<sub>50 </sub>of 25 μg/mL.</p> <p>Conclusions</p> <p>These findings justify the use of <it>H. lanceolatum </it>stem bark as anti-malarial by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new drug candidates or phytomedicines for malaria.</p> |
| first_indexed | 2024-12-13T12:16:58Z |
| format | Article |
| id | doaj.art-811fe066ec9046768541114e14534c22 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-13T12:16:58Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-811fe066ec9046768541114e14534c222022-12-21T23:46:42ZengBMCMalaria Journal1475-28752011-06-0110116710.1186/1475-2875-10-167<it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem barkTane PierreNgemenya Moses NWabo Hippolyte KKowa Théodora KZofou DenisTitanji Vincent PK<p>Abstract</p> <p>Background</p> <p>Malaria is a major public health threat in Africa, and traditional medicine continues to play a key role in its control especially in rural areas. A bioassay-guided fractionation was carried out in order to evaluate the anti-malarial potential and the safety of the methanol extract of the <it>Hypericum lanceolatum </it>stem bark.</p> <p>Methods</p> <p>The anti-plasmodial activity was assayed by the lactate dehydrogenase method (pLDH) against the multidrug-resistant W2mef laboratory strain, and a field isolate (SHF4) of <it>Plasmodium falciparum</it>. Cytotoxicity tests were carried out using the LLC-MK2 monkey kidney epithelial cells.</p> <p>Results</p> <p>Five compounds were isolated from the most active and least cytotoxic ethylacetate sub-extract: betulinic acid (HLT1), 2,2',5,6'-tetrahydroxybenzophenone (HLT2), 5-hydroxy-3-methoxyxanthone (HLT3), 3-hydroxy-5-methoxyxanthone (HLT4) and HLT0 (yet to be identified). Three of the tested compounds presented significant anti-plasmodial activities (with 50% inhibitory concentration, IC<sub>50 </sub>< 5 μM), with 5-hydroxy-3-methoxyxanthone exerting the highest activity, followed by HLT0 and betulinic acid. All the compounds with significant anti-plasmodial activity were non-cytotoxic, except betulinic acid which showed a 50% cytotoxic concentration, CC<sub>50 </sub>of 25 μg/mL.</p> <p>Conclusions</p> <p>These findings justify the use of <it>H. lanceolatum </it>stem bark as anti-malarial by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new drug candidates or phytomedicines for malaria.</p>http://www.malariajournal.com/content/10/1/167 |
| spellingShingle | Tane Pierre Ngemenya Moses N Wabo Hippolyte K Kowa Théodora K Zofou Denis Titanji Vincent PK <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark Malaria Journal |
| title | <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark |
| title_full | <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark |
| title_fullStr | <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark |
| title_full_unstemmed | <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark |
| title_short | <it>Hypericum lanceolatum </it>(Hypericaceae) as a potential source of new anti-malarial agents: a bioassay-guided fractionation of the stem bark |
| title_sort | it hypericum lanceolatum it hypericaceae as a potential source of new anti malarial agents a bioassay guided fractionation of the stem bark |
| url | http://www.malariajournal.com/content/10/1/167 |
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