Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats
Background: Differentiation of transient ischaemic attack (TIA) from ischaemic stroke within the thrombolysis time window is difficult. Although TIA may be diagnosed within this window, the latest imaging technologies are complex and costly. Serum markers, which are non-invasive, rapid and economic,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fnagi.2018.00024/full |
| _version_ | 1819038386508267520 |
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| author | Dong-Bin Li Jing-Li Liu Wei Wang Xiu-Mei Luo Xia Zhou Jin-Pin Li Xiao-Li Cao Xiao-Hong Long Jia-Gui Chen Chao Qin |
| author_facet | Dong-Bin Li Jing-Li Liu Wei Wang Xiu-Mei Luo Xia Zhou Jin-Pin Li Xiao-Li Cao Xiao-Hong Long Jia-Gui Chen Chao Qin |
| author_sort | Dong-Bin Li |
| collection | DOAJ |
| description | Background: Differentiation of transient ischaemic attack (TIA) from ischaemic stroke within the thrombolysis time window is difficult. Although TIA may be diagnosed within this window, the latest imaging technologies are complex and costly. Serum markers, which are non-invasive, rapid and economic, are used for diagnosis and prognosis of various diseases. Exosome-derived miRNA markers for TIA are unknown.Methods: We examined focal brain ischaemia produced by occlusion of the middle cerebral artery (MCAo) for 5 min, 10 min, and 2 h in rats. Exosomal miRNAs with consistent trends in cerebrospinal fluid (CSF) and plasma were identified by deep sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). The areas under the curve (AUC) of the receiver operating characteristic (ROC) curve were used to evaluate the diagnostic accuracy of these miRNAs for TIA in rats.Results: Rno-miR-122-5p and rno-miR-300-3p were selected. Plasma exosomal rno-miR-122-5p was significantly downregulated in 10 min ischaemic rats compared with control and 5 min plasma. Plasma exosomal rno-miR-300-3p was significantly upregulated in 5 min ischaemic rats compared with control, 10 min and 2 h rats. Plasma and CSF levels of these miRNAs were correlated. ROC analysis showed high AUC values for rno-miR-122-5p (0.960) and rno-miR-300-3p (0.970) in the 10 and 5 min rats, respectively, compared with controls.Conclusions: Plasma exosomal rno-miR-122-5p and rno-miR-300-3p may be blood-based TIA biomarkers. |
| first_indexed | 2024-12-21T08:36:29Z |
| format | Article |
| id | doaj.art-8125ee55b2fc49c784c771a9a5ffcf6c |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-12-21T08:36:29Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-8125ee55b2fc49c784c771a9a5ffcf6c2022-12-21T19:10:03ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652018-02-011010.3389/fnagi.2018.00024325218Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in RatsDong-Bin Li0Jing-Li Liu1Wei Wang2Xiu-Mei Luo3Xia Zhou4Jin-Pin Li5Xiao-Li Cao6Xiao-Hong Long7Jia-Gui Chen8Chao Qin9Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaPublic Health School of Guangxi Medical University, Nanning, ChinaDepartment of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, ChinaBackground: Differentiation of transient ischaemic attack (TIA) from ischaemic stroke within the thrombolysis time window is difficult. Although TIA may be diagnosed within this window, the latest imaging technologies are complex and costly. Serum markers, which are non-invasive, rapid and economic, are used for diagnosis and prognosis of various diseases. Exosome-derived miRNA markers for TIA are unknown.Methods: We examined focal brain ischaemia produced by occlusion of the middle cerebral artery (MCAo) for 5 min, 10 min, and 2 h in rats. Exosomal miRNAs with consistent trends in cerebrospinal fluid (CSF) and plasma were identified by deep sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). The areas under the curve (AUC) of the receiver operating characteristic (ROC) curve were used to evaluate the diagnostic accuracy of these miRNAs for TIA in rats.Results: Rno-miR-122-5p and rno-miR-300-3p were selected. Plasma exosomal rno-miR-122-5p was significantly downregulated in 10 min ischaemic rats compared with control and 5 min plasma. Plasma exosomal rno-miR-300-3p was significantly upregulated in 5 min ischaemic rats compared with control, 10 min and 2 h rats. Plasma and CSF levels of these miRNAs were correlated. ROC analysis showed high AUC values for rno-miR-122-5p (0.960) and rno-miR-300-3p (0.970) in the 10 and 5 min rats, respectively, compared with controls.Conclusions: Plasma exosomal rno-miR-122-5p and rno-miR-300-3p may be blood-based TIA biomarkers.http://journal.frontiersin.org/article/10.3389/fnagi.2018.00024/fulltransient ischaemic attackplasma exosomesbiomarkerrno-miR-122-5prno-miR-300-3p |
| spellingShingle | Dong-Bin Li Jing-Li Liu Wei Wang Xiu-Mei Luo Xia Zhou Jin-Pin Li Xiao-Li Cao Xiao-Hong Long Jia-Gui Chen Chao Qin Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats Frontiers in Aging Neuroscience transient ischaemic attack plasma exosomes biomarker rno-miR-122-5p rno-miR-300-3p |
| title | Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats |
| title_full | Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats |
| title_fullStr | Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats |
| title_full_unstemmed | Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats |
| title_short | Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats |
| title_sort | plasma exosomal mirna 122 5p and mir 300 3p as potential markers for transient ischaemic attack in rats |
| topic | transient ischaemic attack plasma exosomes biomarker rno-miR-122-5p rno-miR-300-3p |
| url | http://journal.frontiersin.org/article/10.3389/fnagi.2018.00024/full |
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