Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of...
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MDPI AG
2015-04-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/6/2/192 |
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| author | Jana Lisková Lucie Bačaková Agata L. Skwarczyńska Olga Musial Vitaliy Bliznuk Karel De Schamphelaere Zofia Modrzejewska Timothy E.L. Douglas |
| author_facet | Jana Lisková Lucie Bačaková Agata L. Skwarczyńska Olga Musial Vitaliy Bliznuk Karel De Schamphelaere Zofia Modrzejewska Timothy E.L. Douglas |
| author_sort | Jana Lisková |
| collection | DOAJ |
| description | Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit. |
| first_indexed | 2024-12-10T06:53:53Z |
| format | Article |
| id | doaj.art-8128a6d903ec4f8fa2b2931c588b0d65 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-12-10T06:53:53Z |
| publishDate | 2015-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-8128a6d903ec4f8fa2b2931c588b0d652022-12-22T01:58:28ZengMDPI AGJournal of Functional Biomaterials2079-49832015-04-016219220310.3390/jfb6020192jfb6020192Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration ApplicationsJana Lisková0Lucie Bačaková1Agata L. Skwarczyńska2Olga Musial3Vitaliy Bliznuk4Karel De Schamphelaere5Zofia Modrzejewska6Timothy E.L. Douglas7Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech RepublicDepartment of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Prague, Czech RepublicFaculty of Civil Environmental Engineering and Architecture, Rzeszow University of Technology, ul. Poznanska 2, Rzeszow 35-084, PolandPolymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, Ghent University, Krijgslaan 281 S4, Gent 9000, BelgiumDepartment Material Science & Engineering, Ghent University, Technologiepark 903, Zwijnaarde 9052, BelgiumLaboratory for Environmental and Aquatic Ecology, Environmental Toxicology Unit (GhEnToxLab), Faculty of Bioscience Engineering, Ghent University, Jozef Plateaustraat 22, Gent 9000, BelgiumDepartment of Environmental Systems Engineering, Faculty of Process and Environmental Engineering, Technical University of Łódź, ul. Wólczańska 213, Łódź 90-924, PolandPolymer Chemistry and Biomaterials (PBM) Group, Department of Organic Chemistry, Ghent University, Krijgslaan 281 S4, Gent 9000, BelgiumThermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.http://www.mdpi.com/2079-4983/6/2/192chitosanhydrogelcytocompatibilitymagnesiummineralization |
| spellingShingle | Jana Lisková Lucie Bačaková Agata L. Skwarczyńska Olga Musial Vitaliy Bliznuk Karel De Schamphelaere Zofia Modrzejewska Timothy E.L. Douglas Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications Journal of Functional Biomaterials chitosan hydrogel cytocompatibility magnesium mineralization |
| title | Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications |
| title_full | Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications |
| title_fullStr | Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications |
| title_full_unstemmed | Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications |
| title_short | Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications |
| title_sort | development of thermosensitive hydrogels of chitosan sodium and magnesium glycerophosphate for bone regeneration applications |
| topic | chitosan hydrogel cytocompatibility magnesium mineralization |
| url | http://www.mdpi.com/2079-4983/6/2/192 |
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