Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells.
Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biologi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0267653 |
| _version_ | 1818478786834857984 |
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| author | Renata Faria de Carvalho Lucas de Siqueira Penna Quintaes Thaís de Cássia de Souza Su Leticia Mitiko Kobayashi Ana Cristina Martins de Almeida Nogueira |
| author_facet | Renata Faria de Carvalho Lucas de Siqueira Penna Quintaes Thaís de Cássia de Souza Su Leticia Mitiko Kobayashi Ana Cristina Martins de Almeida Nogueira |
| author_sort | Renata Faria de Carvalho |
| collection | DOAJ |
| description | Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial dengue vaccine and test the feasibility of this approach in the development of a potency assay with human cell lines, as a methodological alternative to the golden standard potency assay with VERO cells. In this context, we used a batch of the commercial vaccine Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined by immunocolorimetric assay using monoclonal antibodies specific for each serotype. The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine, as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to the one observed with VERO cells. Moreover, it was possible to determine the infectivity and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing over the others. Thus, the human cell line HEK293T provides a potential candidate to be used in assays to determine potency and identity of tetravalent dengue vaccines. |
| first_indexed | 2024-12-10T09:52:27Z |
| format | Article |
| id | doaj.art-812ac776578144b5b7928043444eb67c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:52:27Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-812ac776578144b5b7928043444eb67c2022-12-22T01:53:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01175e026765310.1371/journal.pone.0267653Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells.Renata Faria de CarvalhoLucas de Siqueira Penna QuintaesThaís de Cássia de Souza SuLeticia Mitiko KobayashiAna Cristina Martins de Almeida NogueiraDengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial dengue vaccine and test the feasibility of this approach in the development of a potency assay with human cell lines, as a methodological alternative to the golden standard potency assay with VERO cells. In this context, we used a batch of the commercial vaccine Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined by immunocolorimetric assay using monoclonal antibodies specific for each serotype. The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine, as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to the one observed with VERO cells. Moreover, it was possible to determine the infectivity and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing over the others. Thus, the human cell line HEK293T provides a potential candidate to be used in assays to determine potency and identity of tetravalent dengue vaccines.https://doi.org/10.1371/journal.pone.0267653 |
| spellingShingle | Renata Faria de Carvalho Lucas de Siqueira Penna Quintaes Thaís de Cássia de Souza Su Leticia Mitiko Kobayashi Ana Cristina Martins de Almeida Nogueira Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. PLoS ONE |
| title | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. |
| title_full | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. |
| title_fullStr | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. |
| title_full_unstemmed | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. |
| title_short | Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells. |
| title_sort | short communication feasibility of dengue vaccine to infect different human cell lines an alternative potency test using hek293t cells |
| url | https://doi.org/10.1371/journal.pone.0267653 |
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