Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method
Background: Vulvovaginal candidiasis (VVC) is a common vaginal infection, affecting up to 75% of women in child-bearing age. Approximately 5% of patients may experience recurrent VVC. Candida albicans is the most common causative agent with a prevalence of approximately 70–90%. Non-albicans Candida...
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Isfahan University of Medical Sciences
2012-02-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/1269 |
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| author | Rasoul Mohammadi Mehdi Nazeri Elaheh Mesdaghinia Seyed Hossein Mirhendi |
| author_facet | Rasoul Mohammadi Mehdi Nazeri Elaheh Mesdaghinia Seyed Hossein Mirhendi |
| author_sort | Rasoul Mohammadi |
| collection | DOAJ |
| description | Background: Vulvovaginal candidiasis (VVC) is a common vaginal infection, affecting up to 75% of women in child-bearing age. Approximately 5% of patients may experience recurrent VVC. Candida albicans is the most common causative agent with a prevalence of approximately 70–90%. Non-albicans Candida species, predominantly Candida glabrata, are responsible for the remainder. Precise diagnosis of causative agents of VVC is necessary for epidemiological purposes and for effective treatment.
Methods: One hundred and thirty three yeast isolates were collected from a gynecology clinic affiliated to Kashan University, Iran, during 2007-2009. Genomic DNA of each isolate was extracted from fresh cultures using FTA-card. The ITS1-ITS2 region was amplified by polymerase chain reaction (PCR) assay and was digested by the restriction enzyme HpaII. The products of restriction fragment length polymorphism (RFLP) were electrophoresed on agarose gel and the yeast species were determined according to differences in their electrophoretic band patterns.
Findings: Prominent species isolated from clinical specimens were Candida albicans responsible for 116 cases (87.2%), followed by 16 cases of C. glabrata (12%) and 1 case of C. kefyr (0.7%). The age bracket of 31-40 years had the highest and the age bracket of 11-20 years had the lowest frequency of occurrence.
Conclusion: Like the majority of similar studies performed in this field, the present study found Candida albicans as predominant species isolated from vulvovaginal candidiasis. As molecular diagnostic methods are rapid and reliable and as there are no any other studies that used PCR-RFLP for diagnosis of etiologic agents of VVC in Iran, we recommend this approach for other similar epidemiological studies. |
| first_indexed | 2024-03-12T20:15:39Z |
| format | Article |
| id | doaj.art-81303a0a2f194382b9f8229af2eee8a7 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T20:15:39Z |
| publishDate | 2012-02-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-81303a0a2f194382b9f8229af2eee8a72023-08-02T01:23:16ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2012-02-012916522302237816Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP MethodRasoul Mohammadi0Mehdi Nazeri1Elaheh Mesdaghinia2Seyed Hossein Mirhendi3Department of Medical Mycology, School of Medicine, Tarbiat Modares University, Tehran, IranDepartment of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, IranAssistant Professor, Department of Obstetrics and Gynecology and Trauma Research Center, Kashan University of Medical Sciences, Kashan, IranAssociate Professor, Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, IranBackground: Vulvovaginal candidiasis (VVC) is a common vaginal infection, affecting up to 75% of women in child-bearing age. Approximately 5% of patients may experience recurrent VVC. Candida albicans is the most common causative agent with a prevalence of approximately 70–90%. Non-albicans Candida species, predominantly Candida glabrata, are responsible for the remainder. Precise diagnosis of causative agents of VVC is necessary for epidemiological purposes and for effective treatment. Methods: One hundred and thirty three yeast isolates were collected from a gynecology clinic affiliated to Kashan University, Iran, during 2007-2009. Genomic DNA of each isolate was extracted from fresh cultures using FTA-card. The ITS1-ITS2 region was amplified by polymerase chain reaction (PCR) assay and was digested by the restriction enzyme HpaII. The products of restriction fragment length polymorphism (RFLP) were electrophoresed on agarose gel and the yeast species were determined according to differences in their electrophoretic band patterns. Findings: Prominent species isolated from clinical specimens were Candida albicans responsible for 116 cases (87.2%), followed by 16 cases of C. glabrata (12%) and 1 case of C. kefyr (0.7%). The age bracket of 31-40 years had the highest and the age bracket of 11-20 years had the lowest frequency of occurrence. Conclusion: Like the majority of similar studies performed in this field, the present study found Candida albicans as predominant species isolated from vulvovaginal candidiasis. As molecular diagnostic methods are rapid and reliable and as there are no any other studies that used PCR-RFLP for diagnosis of etiologic agents of VVC in Iran, we recommend this approach for other similar epidemiological studies.http://jims.mui.ac.ir/index.php/jims/article/view/1269CandidaVulvovaginitisPCR-RFLP |
| spellingShingle | Rasoul Mohammadi Mehdi Nazeri Elaheh Mesdaghinia Seyed Hossein Mirhendi Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method مجله دانشکده پزشکی اصفهان Candida Vulvovaginitis PCR-RFLP |
| title | Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method |
| title_full | Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method |
| title_fullStr | Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method |
| title_full_unstemmed | Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method |
| title_short | Identification of Candida Species among Patients with Vulvovaginal Candidiasis in Kashan by PCR-RFLP Method |
| title_sort | identification of candida species among patients with vulvovaginal candidiasis in kashan by pcr rflp method |
| topic | Candida Vulvovaginitis PCR-RFLP |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/1269 |
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