Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study
The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/24/4434 |
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| author | Maria António Tânia Lima Rui Vitorino Ana L. Daniel-da-Silva |
| author_facet | Maria António Tânia Lima Rui Vitorino Ana L. Daniel-da-Silva |
| author_sort | Maria António |
| collection | DOAJ |
| description | The use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were incubated with saliva or urine. After the interaction, the surface of gold nanoparticles was investigated using UV-VIS spectroscopy, zeta potential, and dynamic light scattering. The shifting of the localized surface plasmon resonance (LSPR) band, the increase in hydrodynamic diameter, and the changes in the surface charge of nanoparticles indicated the presence of biomolecules on the surface of AuNSs, AuNRs, and AuNFs. The incubation of AuNFs with saliva led to nanoparticle aggregation and minimal protein adsorption. AuNSs and AuNRs incubated in saliva were analyzed through liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the 96 proteins adsorbed on the surface of the gold nanoparticles. Among the 20 most abundant proteins identified, 14 proteins were common in both AuNSs and AuNRs. We hypothesize that the adsorption of these proteins was due to their high sulfur content, allowing for their interaction with gold nanoparticles via the Au-S bond. The presence of distinct proteins on the surface of AuNSs or AuNRs was also investigated and possibly related to the competition between proteins present on the external layers of corona and gold nanoparticle morphology. |
| first_indexed | 2024-03-09T16:01:24Z |
| format | Article |
| id | doaj.art-81318063bd3944c183491dbc6fc1eed2 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T16:01:24Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-81318063bd3944c183491dbc6fc1eed22023-11-24T17:04:18ZengMDPI AGNanomaterials2079-49912022-12-011224443410.3390/nano12244434Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory StudyMaria António0Tânia Lima1Rui Vitorino2Ana L. Daniel-da-Silva3CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugaliBiMED-Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugaliBiMED-Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, PortugalCICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalThe use of gold nanoparticles for drug delivery, photothermal or photodynamic therapy, and biosensing enhances the demand for knowledge about the protein corona formed on the surface of nanoparticles. In this study, gold nanospheres (AuNSs), gold nanorods (AuNRs), and gold nanoflowers (AuNFs) were incubated with saliva or urine. After the interaction, the surface of gold nanoparticles was investigated using UV-VIS spectroscopy, zeta potential, and dynamic light scattering. The shifting of the localized surface plasmon resonance (LSPR) band, the increase in hydrodynamic diameter, and the changes in the surface charge of nanoparticles indicated the presence of biomolecules on the surface of AuNSs, AuNRs, and AuNFs. The incubation of AuNFs with saliva led to nanoparticle aggregation and minimal protein adsorption. AuNSs and AuNRs incubated in saliva were analyzed through liquid chromatography with tandem mass spectrometry (LC-MS/MS) to identify the 96 proteins adsorbed on the surface of the gold nanoparticles. Among the 20 most abundant proteins identified, 14 proteins were common in both AuNSs and AuNRs. We hypothesize that the adsorption of these proteins was due to their high sulfur content, allowing for their interaction with gold nanoparticles via the Au-S bond. The presence of distinct proteins on the surface of AuNSs or AuNRs was also investigated and possibly related to the competition between proteins present on the external layers of corona and gold nanoparticle morphology.https://www.mdpi.com/2079-4991/12/24/4434gold nanoparticlesprotein adsorptionprotein coronasalivaurineLC-MS/MS |
| spellingShingle | Maria António Tânia Lima Rui Vitorino Ana L. Daniel-da-Silva Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study Nanomaterials gold nanoparticles protein adsorption protein corona saliva urine LC-MS/MS |
| title | Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study |
| title_full | Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study |
| title_fullStr | Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study |
| title_full_unstemmed | Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study |
| title_short | Interaction of Colloidal Gold Nanoparticles with Urine and Saliva Biofluids: An Exploratory Study |
| title_sort | interaction of colloidal gold nanoparticles with urine and saliva biofluids an exploratory study |
| topic | gold nanoparticles protein adsorption protein corona saliva urine LC-MS/MS |
| url | https://www.mdpi.com/2079-4991/12/24/4434 |
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