Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal
Lumpy skin disease (LSD) is a transboundary viral disease of cattle and buffaloes transmitted by blood-feeding vectors and causes high morbidity and low-to-moderate mortality. Since the first observation of LSD in Zambia in 1929, it has spread in cattle populations across African countries, the Midd...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/3/539 |
| _version_ | 1797444718317010944 |
|---|---|
| author | Pragya Koirala Irene Kasindi Meki Manju Maharjan Bharani Kumar Settypalli Salina Manandhar Sanjay Kumar Yadav Giovanni Cattoli Charles Euloge Lamien |
| author_facet | Pragya Koirala Irene Kasindi Meki Manju Maharjan Bharani Kumar Settypalli Salina Manandhar Sanjay Kumar Yadav Giovanni Cattoli Charles Euloge Lamien |
| author_sort | Pragya Koirala |
| collection | DOAJ |
| description | Lumpy skin disease (LSD) is a transboundary viral disease of cattle and buffaloes transmitted by blood-feeding vectors and causes high morbidity and low-to-moderate mortality. Since the first observation of LSD in Zambia in 1929, it has spread in cattle populations across African countries, the Middle East, Europe, and Asia. Following the recent outbreaks of LSD in South Asian countries such as India and Bangladesh, the disease was first reported in cattle farms in Nepal in June 2020. This study investigated the Nepalese LSD outbreak and confirmed that the disease spread rapidly to three neighboring districts in a month, infecting 1300 animals. Both cattle and buffaloes showed common clinical signs of LSD, with the exception that the buffaloes presented small nodular lesions without centered ulcerations. The collected samples were first tested for the presence of LSDV by real-time PCR. We further applied molecular tools, RPO30, GPCR, EEV glycoprotein gene, and B22R, for additional characterization of the LSDV isolates circulating in Nepal. Using a PCR-based Snapback assay, we confirmed that samples collected from cattle and buffaloes were positive of LSDV. Furthermore, sequence analysis (phylogenetic and multiple sequence alignments) of four selected LSDV genes revealed that the Nepal LSDVs resemble the Bangladesh and Indian isolates and the historic isolates from Kenya. We also highlight the importance of a unique B22R gene region harboring single-nucleotide insertions in LSDV Neethling and LSDV KSGPO-240 vaccine strains, enabling us to differentiate them from the Nepalese isolates and other fields isolates. This study demonstrates the importance of disease surveillance and the need to determine the source of the disease introduction, the extent of spread, modes of transmission, and the necessary control measures. |
| first_indexed | 2024-03-09T13:16:32Z |
| format | Article |
| id | doaj.art-813317dda21b47ac9439ba235fa372d5 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:32Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-813317dda21b47ac9439ba235fa372d52023-11-30T21:35:45ZengMDPI AGMicroorganisms2076-26072022-02-0110353910.3390/microorganisms10030539Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in NepalPragya Koirala0Irene Kasindi Meki1Manju Maharjan2Bharani Kumar Settypalli3Salina Manandhar4Sanjay Kumar Yadav5Giovanni Cattoli6Charles Euloge Lamien7Central Veterinary Laboratory, Veterinary Complex, Tripureshwor, Kathmandu 44600, NepalAnimal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaCentral Veterinary Laboratory, Veterinary Complex, Tripureshwor, Kathmandu 44600, NepalAnimal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaCentral Veterinary Laboratory, Veterinary Complex, Tripureshwor, Kathmandu 44600, NepalVeterinary Laboratory, Biratnagar, Morang 56613, NepalAnimal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaAnimal Production and Health Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, AustriaLumpy skin disease (LSD) is a transboundary viral disease of cattle and buffaloes transmitted by blood-feeding vectors and causes high morbidity and low-to-moderate mortality. Since the first observation of LSD in Zambia in 1929, it has spread in cattle populations across African countries, the Middle East, Europe, and Asia. Following the recent outbreaks of LSD in South Asian countries such as India and Bangladesh, the disease was first reported in cattle farms in Nepal in June 2020. This study investigated the Nepalese LSD outbreak and confirmed that the disease spread rapidly to three neighboring districts in a month, infecting 1300 animals. Both cattle and buffaloes showed common clinical signs of LSD, with the exception that the buffaloes presented small nodular lesions without centered ulcerations. The collected samples were first tested for the presence of LSDV by real-time PCR. We further applied molecular tools, RPO30, GPCR, EEV glycoprotein gene, and B22R, for additional characterization of the LSDV isolates circulating in Nepal. Using a PCR-based Snapback assay, we confirmed that samples collected from cattle and buffaloes were positive of LSDV. Furthermore, sequence analysis (phylogenetic and multiple sequence alignments) of four selected LSDV genes revealed that the Nepal LSDVs resemble the Bangladesh and Indian isolates and the historic isolates from Kenya. We also highlight the importance of a unique B22R gene region harboring single-nucleotide insertions in LSDV Neethling and LSDV KSGPO-240 vaccine strains, enabling us to differentiate them from the Nepalese isolates and other fields isolates. This study demonstrates the importance of disease surveillance and the need to determine the source of the disease introduction, the extent of spread, modes of transmission, and the necessary control measures.https://www.mdpi.com/2076-2607/10/3/539LSDVNepalsequenceoutbreak |
| spellingShingle | Pragya Koirala Irene Kasindi Meki Manju Maharjan Bharani Kumar Settypalli Salina Manandhar Sanjay Kumar Yadav Giovanni Cattoli Charles Euloge Lamien Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal Microorganisms LSDV Nepal sequence outbreak |
| title | Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal |
| title_full | Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal |
| title_fullStr | Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal |
| title_full_unstemmed | Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal |
| title_short | Molecular Characterization of the 2020 Outbreak of Lumpy Skin Disease in Nepal |
| title_sort | molecular characterization of the 2020 outbreak of lumpy skin disease in nepal |
| topic | LSDV Nepal sequence outbreak |
| url | https://www.mdpi.com/2076-2607/10/3/539 |
| work_keys_str_mv | AT pragyakoirala molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT irenekasindimeki molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT manjumaharjan molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT bharanikumarsettypalli molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT salinamanandhar molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT sanjaykumaryadav molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT giovannicattoli molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal AT charleseulogelamien molecularcharacterizationofthe2020outbreakoflumpyskindiseaseinnepal |