The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis
The multiple sclerosis (MS) incidence rate has been increasing in Russia, but the information about the gut bacteriobiome in the MS-afflicted patients is scarce. Using the Illumina MiSeq sequencing of 16S rRNA gene amplicons, we aimed to analyze the <i>Firmicutes</i> phylum and its taxa...
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MDPI AG
2021-01-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/1/55 |
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| author | Madina Kozhieva Natalia Naumova Tatiana Alikina Alexey Boyko Valentin Vlassov Marsel R. Kabilov |
| author_facet | Madina Kozhieva Natalia Naumova Tatiana Alikina Alexey Boyko Valentin Vlassov Marsel R. Kabilov |
| author_sort | Madina Kozhieva |
| collection | DOAJ |
| description | The multiple sclerosis (MS) incidence rate has been increasing in Russia, but the information about the gut bacteriobiome in the MS-afflicted patients is scarce. Using the Illumina MiSeq sequencing of 16S rRNA gene amplicons, we aimed to analyze the <i>Firmicutes</i> phylum and its taxa in a cohort of Moscow patients with relapsing-remitting MS, assessing the effects of age, BMI, disease modifying therapy (DMT), disability (EDSS), and gender. Among 1252 identified bacterial OTUs, 857 represented Firmicutes. The phylum was the most abundant also in sequence reads, overall averaging 74 ± 13%. The general linear model (GLM) analysis implicated <i>Firmicutes/Clostridia/Clostridiales/Lachospiraceae/Blautia/Blautia wexlerae</i> as increasing with BMI, and only <i>Lachospiraceae/Blautia/Blautia wexlerae</i> as increasing with age. A marked DMT-related decrease in <i>Firmicutes</i> was observed in females at the phylum, class (<i>Clostridia</i>), and order (<i>Clostridiales</i>) levels. The results of our study implicate DMT and gender as factors shaping the fecal <i>Firmicutes</i> assemblages. Together with the gender-dependent differential MS incidence growth rate in the country, the results suggest the likely involvement of gender-specific pathoecological mechanisms underlying the occurrence of the disease, switching between its phenotypes and response to disease-modifying therapies. Overall, the presented profile of <i>Firmicutes</i> can be used as a reference for more detailed research aimed at elucidating the contribution of this core phylum and its lower taxa into the etiology and progression of relapsing-remitting multiple sclerosis. |
| first_indexed | 2024-03-09T04:48:56Z |
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| language | English |
| last_indexed | 2024-03-09T04:48:56Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-813366b2d2e747dfb3b0ad04ccbbcca52023-12-03T13:12:15ZengMDPI AGLife2075-17292021-01-011115510.3390/life11010055The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple SclerosisMadina Kozhieva0Natalia Naumova1Tatiana Alikina2Alexey Boyko3Valentin Vlassov4Marsel R. Kabilov5Department of Neurology, Neurosurgery and Medical Genetics of the Pirogov Medical University, 117513 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaDepartment of Neuroimmunology of the Federal Center of CVPI, 117513 Moscow, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, RussiaThe multiple sclerosis (MS) incidence rate has been increasing in Russia, but the information about the gut bacteriobiome in the MS-afflicted patients is scarce. Using the Illumina MiSeq sequencing of 16S rRNA gene amplicons, we aimed to analyze the <i>Firmicutes</i> phylum and its taxa in a cohort of Moscow patients with relapsing-remitting MS, assessing the effects of age, BMI, disease modifying therapy (DMT), disability (EDSS), and gender. Among 1252 identified bacterial OTUs, 857 represented Firmicutes. The phylum was the most abundant also in sequence reads, overall averaging 74 ± 13%. The general linear model (GLM) analysis implicated <i>Firmicutes/Clostridia/Clostridiales/Lachospiraceae/Blautia/Blautia wexlerae</i> as increasing with BMI, and only <i>Lachospiraceae/Blautia/Blautia wexlerae</i> as increasing with age. A marked DMT-related decrease in <i>Firmicutes</i> was observed in females at the phylum, class (<i>Clostridia</i>), and order (<i>Clostridiales</i>) levels. The results of our study implicate DMT and gender as factors shaping the fecal <i>Firmicutes</i> assemblages. Together with the gender-dependent differential MS incidence growth rate in the country, the results suggest the likely involvement of gender-specific pathoecological mechanisms underlying the occurrence of the disease, switching between its phenotypes and response to disease-modifying therapies. Overall, the presented profile of <i>Firmicutes</i> can be used as a reference for more detailed research aimed at elucidating the contribution of this core phylum and its lower taxa into the etiology and progression of relapsing-remitting multiple sclerosis.https://www.mdpi.com/2075-1729/11/1/55multiple sclerosisrelapsing-remitting coursefecal bacteriobiome16S rRNA gene amplicon sequencing |
| spellingShingle | Madina Kozhieva Natalia Naumova Tatiana Alikina Alexey Boyko Valentin Vlassov Marsel R. Kabilov The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis Life multiple sclerosis relapsing-remitting course fecal bacteriobiome 16S rRNA gene amplicon sequencing |
| title | The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_full | The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_fullStr | The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_full_unstemmed | The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_short | The Core of Gut Life: <i>Firmicutes</i> Profile in Patients with Relapsing-Remitting Multiple Sclerosis |
| title_sort | core of gut life i firmicutes i profile in patients with relapsing remitting multiple sclerosis |
| topic | multiple sclerosis relapsing-remitting course fecal bacteriobiome 16S rRNA gene amplicon sequencing |
| url | https://www.mdpi.com/2075-1729/11/1/55 |
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