At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shared Science Publishers OG
2014-05-01
|
| Series: | Microbial Cell |
| Subjects: | |
| Online Access: | http://microbialcell.com/researcharticles/at-neutral-ph-the-chronological-lifespan-of-hansenula-polymorpha-increases-upon-enhancing-the-carbon-source-concentrations/ |
| _version_ | 1819178081739341824 |
|---|---|
| author | Adam Kawałek Ida J. van der Klei |
| author_facet | Adam Kawałek Ida J. van der Klei |
| author_sort | Adam Kawałek |
| collection | DOAJ |
| description | Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration on the chronological lifespan of the yeast Hansenula polymorpha using four different carbon sources. Our data indicate that reduction of the carbon source concentration has a negative (glucose, ethanol, methanol) or positive (glycerol) effect on the chronological lifespan. We show that the actual effect of carbon source concentrations largely depends on extracellular factor(s). We provide evidence that H. polymorpha acidifies the medium and that a low pH of the medium alone is sufficient to significantly decrease the chronological lifespan. However, glucose-grown cells are less sensitive to low pH compared to glycerol-grown cells, explaining why only the reduction of the glycerol-concentration (which leads to less medium acidification) has a positive effect on the chronological lifespan. Instead, the positive effect of enhancing the glucose concentrations is much larger than the negative effect of the medium acidification at these conditions, explaining the increased lifespan with increasing glucose concentrations. Importantly, at neutral pH, the chronological lifespan also decreases with a reduction in glycerol concentrations. We show that for glycerol cultures this effect is related to acidification independent changes in the composition of the spent medium. Altogether, our data indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration. |
| first_indexed | 2024-12-22T21:36:53Z |
| format | Article |
| id | doaj.art-81356d538c5d47d2bfbc36b7e42947ab |
| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2024-12-22T21:36:53Z |
| publishDate | 2014-05-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-81356d538c5d47d2bfbc36b7e42947ab2022-12-21T18:11:43ZengShared Science Publishers OGMicrobial Cell2311-26382014-05-011618920210.15698/mic2014.06.149At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrationsAdam Kawałek0Ida J. van der Klei1Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Systems Biology Centre for Metabolism and Ageing, University of Groningen, the Netherlands.Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Systems Biology Centre for Metabolism and Ageing, University of Groningen, the Netherlands.Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration on the chronological lifespan of the yeast Hansenula polymorpha using four different carbon sources. Our data indicate that reduction of the carbon source concentration has a negative (glucose, ethanol, methanol) or positive (glycerol) effect on the chronological lifespan. We show that the actual effect of carbon source concentrations largely depends on extracellular factor(s). We provide evidence that H. polymorpha acidifies the medium and that a low pH of the medium alone is sufficient to significantly decrease the chronological lifespan. However, glucose-grown cells are less sensitive to low pH compared to glycerol-grown cells, explaining why only the reduction of the glycerol-concentration (which leads to less medium acidification) has a positive effect on the chronological lifespan. Instead, the positive effect of enhancing the glucose concentrations is much larger than the negative effect of the medium acidification at these conditions, explaining the increased lifespan with increasing glucose concentrations. Importantly, at neutral pH, the chronological lifespan also decreases with a reduction in glycerol concentrations. We show that for glycerol cultures this effect is related to acidification independent changes in the composition of the spent medium. Altogether, our data indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration.http://microbialcell.com/researcharticles/at-neutral-ph-the-chronological-lifespan-of-hansenula-polymorpha-increases-upon-enhancing-the-carbon-source-concentrations/Hansenula polymorphachronological lifespanageingacidificationdietary restriction |
| spellingShingle | Adam Kawałek Ida J. van der Klei At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations Microbial Cell Hansenula polymorpha chronological lifespan ageing acidification dietary restriction |
| title | At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations |
| title_full | At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations |
| title_fullStr | At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations |
| title_full_unstemmed | At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations |
| title_short | At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations |
| title_sort | at neutral ph the chronological lifespan of hansenula polymorpha increases upon enhancing the carbon source concentrations |
| topic | Hansenula polymorpha chronological lifespan ageing acidification dietary restriction |
| url | http://microbialcell.com/researcharticles/at-neutral-ph-the-chronological-lifespan-of-hansenula-polymorpha-increases-upon-enhancing-the-carbon-source-concentrations/ |
| work_keys_str_mv | AT adamkawałek atneutralphthechronologicallifespanofhansenulapolymorphaincreasesuponenhancingthecarbonsourceconcentrations AT idajvanderklei atneutralphthechronologicallifespanofhansenulapolymorphaincreasesuponenhancingthecarbonsourceconcentrations |