Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study
Abstract Background Building on the declining trend of malaria in Ethiopia, the Federal Ministry of Health aims to eliminate malaria by 2030. As Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia, the use of primaquine is indicated for both transmission interruption and radical cu...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12936-018-2538-4 |
| _version_ | 1811334388127891456 |
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| author | Ashenafi Assefa Ahmed Ali Wakgari Deressa Wendimagegn Tsegaye Getachew Abebe Heven Sime Amha Kebede Daddi Jima Moges Kassa Tesfay Abreha Hiwot Teka Hiwot Solomon Joseph Malone Ya Ping Shi Zhiyong Zhou Richard Reithinger Jimee Hwang |
| author_facet | Ashenafi Assefa Ahmed Ali Wakgari Deressa Wendimagegn Tsegaye Getachew Abebe Heven Sime Amha Kebede Daddi Jima Moges Kassa Tesfay Abreha Hiwot Teka Hiwot Solomon Joseph Malone Ya Ping Shi Zhiyong Zhou Richard Reithinger Jimee Hwang |
| author_sort | Ashenafi Assefa |
| collection | DOAJ |
| description | Abstract Background Building on the declining trend of malaria in Ethiopia, the Federal Ministry of Health aims to eliminate malaria by 2030. As Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia, the use of primaquine is indicated for both transmission interruption and radical cure, respectively. However, the limited knowledge of the local prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its associated variants has hindered the use of primaquine. Methods Some 11,138 dried blood spot (DBS) samples were collected in 2011 as part of a national, household Malaria Indicator Survey, a multi-stage nationally representative survey of all malaria-endemic areas of Ethiopia. A randomly selected sub-set of 1414 DBS samples was successfully genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique. Considering the geographical position and ethnic mix of the country, three common variants: G6PD*A (A376G), G6PD*A− (G202A) and Mediterranean (C563T) were investigated. Results Of the 1998 randomly selected individuals, 1429 (71.5%) DBS samples were genotyped and merged to the database, of which 53.5% were from females. G6PD*A (A376G) was the only genotype detected. No sample was positive for either G6PD*A− (G202A) or Mediterranean (C563T) variants. The prevalence of G6PD*A (A376G) was 8.9% [95% confidence interval (CI) 6.7–11.2] ranging from 12.2% in the Southern Nations, Nationalities and Peoples’ (95% CI 5.7–18.7) to none in Dire Dawa/Harari Region. Conclusion The common G6PD*A− (G202A) or Mediterranean (C563T) variants were not observed in this nationwide study. The observed G6PD*A (A376G) mutation has little or no clinical significance. These findings supported the adoption of primaquine for P. falciparum transmission interruption and radical cure of P. vivax in Ethiopia. As the presence of other clinically important, less common variants cannot be ruled out, the implementation of radical cure will be accompanied by active haematological and adverse events monitoring in Ethiopia. |
| first_indexed | 2024-04-13T17:07:08Z |
| format | Article |
| id | doaj.art-813638eb39ee4193953055ea3be7c202 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-13T17:07:08Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-813638eb39ee4193953055ea3be7c2022022-12-22T02:38:25ZengBMCMalaria Journal1475-28752018-10-011711710.1186/s12936-018-2538-4Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide studyAshenafi Assefa0Ahmed Ali1Wakgari Deressa2Wendimagegn Tsegaye3Getachew Abebe4Heven Sime5Amha Kebede6Daddi Jima7Moges Kassa8Tesfay Abreha9Hiwot Teka10Hiwot Solomon11Joseph Malone12Ya Ping Shi13Zhiyong Zhou14Richard Reithinger15Jimee Hwang16Ethiopian Public Health InstituteAddis Ababa UniversityAddis Ababa UniversityAddis Ababa UniversityAddis Ababa UniversityEthiopian Public Health InstituteEthiopian Public Health InstituteEthiopian Public Health InstituteEthiopian Public Health InstituteColumbia University ICAPUS President’s Malaria Initiative, United States Agency for International DevelopmentEthiopian Federal Ministry of HealthUS President’s Malaria Initiative, Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and PreventionMalaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and PreventionMalaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and PreventionUS President’s Malaria Initiative, United States Agency for International DevelopmentUS President’s Malaria Initiative, Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and PreventionAbstract Background Building on the declining trend of malaria in Ethiopia, the Federal Ministry of Health aims to eliminate malaria by 2030. As Plasmodium falciparum and Plasmodium vivax are co-endemic in Ethiopia, the use of primaquine is indicated for both transmission interruption and radical cure, respectively. However, the limited knowledge of the local prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its associated variants has hindered the use of primaquine. Methods Some 11,138 dried blood spot (DBS) samples were collected in 2011 as part of a national, household Malaria Indicator Survey, a multi-stage nationally representative survey of all malaria-endemic areas of Ethiopia. A randomly selected sub-set of 1414 DBS samples was successfully genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique. Considering the geographical position and ethnic mix of the country, three common variants: G6PD*A (A376G), G6PD*A− (G202A) and Mediterranean (C563T) were investigated. Results Of the 1998 randomly selected individuals, 1429 (71.5%) DBS samples were genotyped and merged to the database, of which 53.5% were from females. G6PD*A (A376G) was the only genotype detected. No sample was positive for either G6PD*A− (G202A) or Mediterranean (C563T) variants. The prevalence of G6PD*A (A376G) was 8.9% [95% confidence interval (CI) 6.7–11.2] ranging from 12.2% in the Southern Nations, Nationalities and Peoples’ (95% CI 5.7–18.7) to none in Dire Dawa/Harari Region. Conclusion The common G6PD*A− (G202A) or Mediterranean (C563T) variants were not observed in this nationwide study. The observed G6PD*A (A376G) mutation has little or no clinical significance. These findings supported the adoption of primaquine for P. falciparum transmission interruption and radical cure of P. vivax in Ethiopia. As the presence of other clinically important, less common variants cannot be ruled out, the implementation of radical cure will be accompanied by active haematological and adverse events monitoring in Ethiopia.http://link.springer.com/article/10.1186/s12936-018-2538-4EthiopiaMalariaG6PD deficiencyPrimaquine |
| spellingShingle | Ashenafi Assefa Ahmed Ali Wakgari Deressa Wendimagegn Tsegaye Getachew Abebe Heven Sime Amha Kebede Daddi Jima Moges Kassa Tesfay Abreha Hiwot Teka Hiwot Solomon Joseph Malone Ya Ping Shi Zhiyong Zhou Richard Reithinger Jimee Hwang Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study Malaria Journal Ethiopia Malaria G6PD deficiency Primaquine |
| title | Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study |
| title_full | Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study |
| title_fullStr | Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study |
| title_full_unstemmed | Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study |
| title_short | Glucose-6-phosphate dehydrogenase (G6PD) deficiency in Ethiopia: absence of common African and Mediterranean allelic variants in a nationwide study |
| title_sort | glucose 6 phosphate dehydrogenase g6pd deficiency in ethiopia absence of common african and mediterranean allelic variants in a nationwide study |
| topic | Ethiopia Malaria G6PD deficiency Primaquine |
| url | http://link.springer.com/article/10.1186/s12936-018-2538-4 |
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