Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism

Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diver...

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Main Author: Xiao Chen
Format: Article
Language:English
Published: Firenze University Press 2022-09-01
Series:Caryologia
Online Access:https://riviste.fupress.net/index.php/caryologia/article/view/1515
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author Xiao Chen
author_facet Xiao Chen
author_sort Xiao Chen
collection DOAJ
description Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through  a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in  Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.
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spelling doaj.art-81379ce0cf824d30a5bac93223792a522022-12-22T03:15:21ZengFirenze University PressCaryologia0008-71142165-53912022-09-0110.36253/caryologia-1515Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism Xiao Chen0Department of Biology, Tehran North Branch, Islamic Azad University Genus Hypericum (Guttiferae, Hypericoideae) is perennial, belonging to the Hypericaceae family, having 484 species in forms of trees, shrubs, and herbs, distributed in 36 taxonomic sections. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Hypericum species, were studied through  a combination of morphological and molecular data. Eighty-five individuals related to 7 Hypericum were collected in 6 provinces. A total of 76 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Hypericum species. These bands were produced with the combinations of 5 selective primers. The total number of amplified fragments ranged from 10 to 20. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, H. perforatum and H. asperulum had the lowest similarity. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in  Hypericum species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran. https://riviste.fupress.net/index.php/caryologia/article/view/1515
spellingShingle Xiao Chen
Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
Caryologia
title Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
title_full Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
title_fullStr Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
title_full_unstemmed Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
title_short Morphometric analysis and genetic diversity in Hypericum L. Using Sequence related amplified polymorphism
title_sort morphometric analysis and genetic diversity in hypericum l using sequence related amplified polymorphism
url https://riviste.fupress.net/index.php/caryologia/article/view/1515
work_keys_str_mv AT xiaochen morphometricanalysisandgeneticdiversityinhypericumlusingsequencerelatedamplifiedpolymorphism