Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy
Abstract Surgical resection remains a critical treatment option for many patients with primary and secondary hepatic neoplasms. Extended hepatectomy (eHx) may be required for some patients with large tumors, which may cause liver failure and death. Partial hepatectomy (pHx) and eHx mouse models were...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09647-0 |
| _version_ | 1797453747233751040 |
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| author | Zeyuan Li Bo Peng Shilian Chen Jiaping Li Kai Hu Lijuan Liao Qiuli Xie Mei Yao Lixing Liang Stephen Tomlinson Guandou Yuan Songqing He |
| author_facet | Zeyuan Li Bo Peng Shilian Chen Jiaping Li Kai Hu Lijuan Liao Qiuli Xie Mei Yao Lixing Liang Stephen Tomlinson Guandou Yuan Songqing He |
| author_sort | Zeyuan Li |
| collection | DOAJ |
| description | Abstract Surgical resection remains a critical treatment option for many patients with primary and secondary hepatic neoplasms. Extended hepatectomy (eHx) may be required for some patients with large tumors, which may cause liver failure and death. Partial hepatectomy (pHx) and eHx mouse models were constructed, liver tissues were sampled at 18, 36, and 72 h posthepatectomy. Transcriptome and metabolome analyses were employed to explore the different potential mechanisms in regeneration and injury between pHx and eHx. The results showed that eHx was associated with more severe liver injury and lower survival rates than pHx. Transcriptomics data showed there were 1842, 2129, and 1277 differentially expressed genes (DEGs) in eHx and 962, 1305, and 732 DEGs in pHx at 18, 36, and 72 h posthepatectomy, respectively, compared with the those in the sham groups. Compared with pHx, the number of DEGs in the eHx group reached a maximum of 230 at 18 h after surgery and decreased sequentially to 87 and 43 at 36 and 72 h. Metabolomics analysis identified a total of 1399 metabolites, and 48 significant differentially produced metabolites (DPMs) were screened between eHx and pHx. Combined analysis of DEGs and DPMs indicated that cholesterol metabolism and insulin resistance may be two important pathways for liver regeneration and mouse survival postextended hepatectomy. Our results showed the global influence of pHx and eHx on the transcriptome and metabolome in mouse liver, and revealed cholesterol metabolism and insulin resistance pathways might be involved in regeneration post-pHx and -eHx. |
| first_indexed | 2024-03-09T15:27:20Z |
| format | Article |
| id | doaj.art-813875ed68504c2691befddd391db447 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:27:20Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-813875ed68504c2691befddd391db4472023-11-26T12:26:11ZengBMCBMC Genomics1471-21642023-09-0124111610.1186/s12864-023-09647-0Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomyZeyuan Li0Bo Peng1Shilian Chen2Jiaping Li3Kai Hu4Lijuan Liao5Qiuli Xie6Mei Yao7Lixing Liang8Stephen Tomlinson9Guandou Yuan10Songqing He11Division of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityGuangxi Key Laboratory of Precision Medicine in Cardio-cerebrovascular Diseases Control and Prevention, the First Affiliated Hospital of Guangxi Medical UniversityGuangxi Key Laboratory of Immunology and Metabolism for Liver Diseases, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityGuangxi Key Laboratory of Immunology and Metabolism for Liver Diseases, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Microbiology and Immunology, Medical University of South CarolinaDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityDivision of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical UniversityAbstract Surgical resection remains a critical treatment option for many patients with primary and secondary hepatic neoplasms. Extended hepatectomy (eHx) may be required for some patients with large tumors, which may cause liver failure and death. Partial hepatectomy (pHx) and eHx mouse models were constructed, liver tissues were sampled at 18, 36, and 72 h posthepatectomy. Transcriptome and metabolome analyses were employed to explore the different potential mechanisms in regeneration and injury between pHx and eHx. The results showed that eHx was associated with more severe liver injury and lower survival rates than pHx. Transcriptomics data showed there were 1842, 2129, and 1277 differentially expressed genes (DEGs) in eHx and 962, 1305, and 732 DEGs in pHx at 18, 36, and 72 h posthepatectomy, respectively, compared with the those in the sham groups. Compared with pHx, the number of DEGs in the eHx group reached a maximum of 230 at 18 h after surgery and decreased sequentially to 87 and 43 at 36 and 72 h. Metabolomics analysis identified a total of 1399 metabolites, and 48 significant differentially produced metabolites (DPMs) were screened between eHx and pHx. Combined analysis of DEGs and DPMs indicated that cholesterol metabolism and insulin resistance may be two important pathways for liver regeneration and mouse survival postextended hepatectomy. Our results showed the global influence of pHx and eHx on the transcriptome and metabolome in mouse liver, and revealed cholesterol metabolism and insulin resistance pathways might be involved in regeneration post-pHx and -eHx.https://doi.org/10.1186/s12864-023-09647-0Partial hepatectomyExtended hepatectomyTranscriptomeMetabolomeRegeneration |
| spellingShingle | Zeyuan Li Bo Peng Shilian Chen Jiaping Li Kai Hu Lijuan Liao Qiuli Xie Mei Yao Lixing Liang Stephen Tomlinson Guandou Yuan Songqing He Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy BMC Genomics Partial hepatectomy Extended hepatectomy Transcriptome Metabolome Regeneration |
| title | Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| title_full | Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| title_fullStr | Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| title_full_unstemmed | Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| title_short | Transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| title_sort | transcriptome sequencing and metabolome analysis reveal the metabolic reprogramming of partial hepatectomy and extended hepatectomy |
| topic | Partial hepatectomy Extended hepatectomy Transcriptome Metabolome Regeneration |
| url | https://doi.org/10.1186/s12864-023-09647-0 |
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