Ascorbic acid improves the tolerance of wheat plants to lead toxicity
Among the heavy metals (HMs), lead (Pb) is considered as a toxic HM which adversely affects growth and development of crop plants. The present experiment was aimed to investigate the potential role of ascorbic acid (ASC) in the reversal of Pb-inhibited nitrogen and sulfur assimilation enzymes activi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-01-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/17429145.2018.1491067 |
| _version_ | 1818149051943616512 |
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| author | Saud A Alamri Manzer H Siddiqui Mutahhar YY Al-Khaishany M. Nasir Khan Hayssam M Ali Ibrahim A. Alaraidh Abdulaziz A. Alsahli Hala Al-Rabiah Mohammed Mateen |
| author_facet | Saud A Alamri Manzer H Siddiqui Mutahhar YY Al-Khaishany M. Nasir Khan Hayssam M Ali Ibrahim A. Alaraidh Abdulaziz A. Alsahli Hala Al-Rabiah Mohammed Mateen |
| author_sort | Saud A Alamri |
| collection | DOAJ |
| description | Among the heavy metals (HMs), lead (Pb) is considered as a toxic HM which adversely affects growth and development of crop plants. The present experiment was aimed to investigate the potential role of ascorbic acid (ASC) in the reversal of Pb-inhibited nitrogen and sulfur assimilation enzymes activity and activity of photosynthesis enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and growth response in wheat plants. Wheat seedlings were subjected to 0 mM (control) and 0.2 mM and 0.6 mM of ASC with and without 2 mM of Pb. Plants treated with Pb exhibited the following reduced growth characteristics (root length, shoot length, root fresh weight (FW), shoot FW, root dry weight (DW) and shoot DW). A decrease was also observed in the activity of Rubisco and ATP sulfurylase (ATP-S), relative water content (RWC), accumulation of total chlorophyll (Total Chl) and content of nutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg)] in Pb-treated plants. However, an increase in Chl degradation and in the activity of O-acetylserine(thiol)lyase (OAS-TL) and accumulation of cysteine (Cys), malondialdehyde (MDA) and hydrogen peroxide (H2O2) was observed in plants under Pb stress. On the contrary, exogenous application of ASC mitigated the Pb-toxicity-induced oxidative damage by enhancing the activities of antioxidant enzymes, such as superoxide dismutase, catalase and glutathione reductase. Improved activity of antioxidant enzymes suppressed the formation of MDA and H2O2, which was reflected in the form of improved growth characteristics. Moreover, ASC induced improvement in plants defense systems by reduced Chl degradation and improved the content of essential nutrients (N, P, K, Ca and Mg) and Cys, RWC and the activity of Rubisco, ATP-S, NR and OAS-TL. |
| first_indexed | 2024-12-11T13:00:54Z |
| format | Article |
| id | doaj.art-8138a3f3fc874adfa1b0a7072cbd309d |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-12-11T13:00:54Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-8138a3f3fc874adfa1b0a7072cbd309d2022-12-22T01:06:28ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532018-01-0113140941910.1080/17429145.2018.14910671491067Ascorbic acid improves the tolerance of wheat plants to lead toxicitySaud A Alamri0Manzer H Siddiqui1Mutahhar YY Al-Khaishany2M. Nasir Khan3Hayssam M Ali4Ibrahim A. Alaraidh5Abdulaziz A. Alsahli6Hala Al-Rabiah7Mohammed Mateen8College of Science, King Saud UniversityCollege of Science, King Saud UniversityCollege of Science, King Saud UniversityUniversity of TabukCollege of Science, King Saud UniversityCollege of Science, King Saud UniversityCollege of Science, King Saud UniversityCollege of Science, King Saud UniversityCollege of Science, King Saud UniversityAmong the heavy metals (HMs), lead (Pb) is considered as a toxic HM which adversely affects growth and development of crop plants. The present experiment was aimed to investigate the potential role of ascorbic acid (ASC) in the reversal of Pb-inhibited nitrogen and sulfur assimilation enzymes activity and activity of photosynthesis enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and growth response in wheat plants. Wheat seedlings were subjected to 0 mM (control) and 0.2 mM and 0.6 mM of ASC with and without 2 mM of Pb. Plants treated with Pb exhibited the following reduced growth characteristics (root length, shoot length, root fresh weight (FW), shoot FW, root dry weight (DW) and shoot DW). A decrease was also observed in the activity of Rubisco and ATP sulfurylase (ATP-S), relative water content (RWC), accumulation of total chlorophyll (Total Chl) and content of nutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg)] in Pb-treated plants. However, an increase in Chl degradation and in the activity of O-acetylserine(thiol)lyase (OAS-TL) and accumulation of cysteine (Cys), malondialdehyde (MDA) and hydrogen peroxide (H2O2) was observed in plants under Pb stress. On the contrary, exogenous application of ASC mitigated the Pb-toxicity-induced oxidative damage by enhancing the activities of antioxidant enzymes, such as superoxide dismutase, catalase and glutathione reductase. Improved activity of antioxidant enzymes suppressed the formation of MDA and H2O2, which was reflected in the form of improved growth characteristics. Moreover, ASC induced improvement in plants defense systems by reduced Chl degradation and improved the content of essential nutrients (N, P, K, Ca and Mg) and Cys, RWC and the activity of Rubisco, ATP-S, NR and OAS-TL.http://dx.doi.org/10.1080/17429145.2018.1491067Ascorbic acidATP sulfurylasechlorophyll degradationleadRubiscoO-acetylserine(thiol)lyase; Triticum aestivum |
| spellingShingle | Saud A Alamri Manzer H Siddiqui Mutahhar YY Al-Khaishany M. Nasir Khan Hayssam M Ali Ibrahim A. Alaraidh Abdulaziz A. Alsahli Hala Al-Rabiah Mohammed Mateen Ascorbic acid improves the tolerance of wheat plants to lead toxicity Journal of Plant Interactions Ascorbic acid ATP sulfurylase chlorophyll degradation lead Rubisco O-acetylserine(thiol)lyase; Triticum aestivum |
| title | Ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| title_full | Ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| title_fullStr | Ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| title_full_unstemmed | Ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| title_short | Ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| title_sort | ascorbic acid improves the tolerance of wheat plants to lead toxicity |
| topic | Ascorbic acid ATP sulfurylase chlorophyll degradation lead Rubisco O-acetylserine(thiol)lyase; Triticum aestivum |
| url | http://dx.doi.org/10.1080/17429145.2018.1491067 |
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