Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model
Summary: Two independent trials were conducted to assess the effect of Herbanoplex CP (HB) on broiler chicken's performance using a 1) a nondefined challenge or 2) necrotic enteritis (NE) challenge model with intestinal lesion scoring. Herbanoplex CP is a unique combination of phytocompounds (g...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Journal of Applied Poultry Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1056617121000246 |
| _version_ | 1818645935194898432 |
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| author | B. Vecchi E. Gumina F. Matte A. Bata S. Bata V. Molnar-Nagy J.W. Hall X. Hernandez-Velasco S. Layton |
| author_facet | B. Vecchi E. Gumina F. Matte A. Bata S. Bata V. Molnar-Nagy J.W. Hall X. Hernandez-Velasco S. Layton |
| author_sort | B. Vecchi |
| collection | DOAJ |
| description | Summary: Two independent trials were conducted to assess the effect of Herbanoplex CP (HB) on broiler chicken's performance using a 1) a nondefined challenge or 2) necrotic enteritis (NE) challenge model with intestinal lesion scoring. Herbanoplex CP is a unique combination of phytocompounds (ground hops, wheat germ, and chicory). In Exp 1, 400-day-old male chicks (Cobb 500) were individually tagged, weighed, and randomly assigned to one of 20 pens (n = 20 birds/pen). Pens were randomly assigned per treatment group: 4 treatments with 5 replicates each (n = 100 birds/treatment). T1: no modification; T2: antibiotic growth promoter bacitracin methylene disalicylate (BMD) 11% at 0.5 kg/metric ton); T3: HB 1 kg/metric ton; and T4: HB at 0.5 kg/metric ton. On day 7, 14, 15 of life, all birds in all treatment groups were challenged by drinking water with a liter filtrate as a nondefined challenge model. Their performance was evaluated at day 35. T3 had a significant (P-value < 0.05) increase in body weight gain (BWG) and a significant reduction in FCR compared with the rest of the experimental groups. Interestingly, chickens in T2, showed similar BWG and FCR when compared with chickens in T4. In Exp 2, 100-day-old male broiler chicks were divided into 5 groups and allocated in isolation cages. T1: birds without Clostridium perfringens (CP) challenge; T2: birds with CP challenge; T3: birds with CP challenge plus HB at 1 kg/metric ton; T4: birds with CP challenge plus HB at 0.75 kg/metric ton and T5: birds with CP challenge plus HB at 0.5 kg/metric ton. At day 13, all chickens were vaccinated with 10x coccidia oocysts. At day 14, all chickens were treated with a 10x dose of infectious bursal disease vaccine. From day 15 to day 19, chickens in challenge groups (T2, T3, T4, and T5) were orally gavaged with 108 cfu of C. perfringens twice a day. At day 21, all chickens were euthanized, and NE intestinal lesion scores were recorded. Chickens in T3 and T4 exposed to a NE challenge model showed significantly reduced lesion scores compared with the rest of the groups. Furthermore, in vitro minimal inhibitory concentration (MIC) assays revealed that HB had the lowest MIC at both 24 h (390 μg/mL) and 48 h (625 μg/mL) against C. perfringens. Herbanoplex CP showed similar response to BMD in a nonspecific challenge model in promoting growth performance (Exp 1). Moreover, HB reduced the severity of intestinal lesion score using a NE challenge model (Exp 2), suggesting that the antibacterial properties against C. perfringens by HB can promote growth during microbial challenges. |
| first_indexed | 2024-12-17T00:38:39Z |
| format | Article |
| id | doaj.art-813a2d35d5c84d6faf3f664e38db6966 |
| institution | Directory Open Access Journal |
| issn | 1056-6171 |
| language | English |
| last_indexed | 2024-12-17T00:38:39Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Applied Poultry Research |
| spelling | doaj.art-813a2d35d5c84d6faf3f664e38db69662022-12-21T22:10:05ZengElsevierJournal of Applied Poultry Research1056-61712021-06-01302100161Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge modelB. Vecchi0E. Gumina1F. Matte2A. Bata3S. Bata4V. Molnar-Nagy5J.W. Hall6X. Hernandez-Velasco7S. Layton8Department of Biotechnology, Vetanco SA, Provincia de Buenos Aires, ArgentinaDepartment of Biotechnology, Vetanco SA, Provincia de Buenos Aires, ArgentinaDepartment of Biotechnology, Vetanco SA, Provincia de Buenos Aires, ArgentinaDepartment of Research and Development, Dr. Bata Ltd., HungríaDepartment of Research and Development, Dr. Bata Ltd., HungríaDepartment of Research and Development, Dr. Bata Ltd., HungríaDepartment of Research and Development, Vetanco USA, Inc., Saint Paul, MN, USADepartamento de Medicina y Zootecnia de Aves, FMVZ, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico 04510, MexicoDepartment of Research and Development, BV Science, Lenexa, KS 66219, USA; Corresponding author:Summary: Two independent trials were conducted to assess the effect of Herbanoplex CP (HB) on broiler chicken's performance using a 1) a nondefined challenge or 2) necrotic enteritis (NE) challenge model with intestinal lesion scoring. Herbanoplex CP is a unique combination of phytocompounds (ground hops, wheat germ, and chicory). In Exp 1, 400-day-old male chicks (Cobb 500) were individually tagged, weighed, and randomly assigned to one of 20 pens (n = 20 birds/pen). Pens were randomly assigned per treatment group: 4 treatments with 5 replicates each (n = 100 birds/treatment). T1: no modification; T2: antibiotic growth promoter bacitracin methylene disalicylate (BMD) 11% at 0.5 kg/metric ton); T3: HB 1 kg/metric ton; and T4: HB at 0.5 kg/metric ton. On day 7, 14, 15 of life, all birds in all treatment groups were challenged by drinking water with a liter filtrate as a nondefined challenge model. Their performance was evaluated at day 35. T3 had a significant (P-value < 0.05) increase in body weight gain (BWG) and a significant reduction in FCR compared with the rest of the experimental groups. Interestingly, chickens in T2, showed similar BWG and FCR when compared with chickens in T4. In Exp 2, 100-day-old male broiler chicks were divided into 5 groups and allocated in isolation cages. T1: birds without Clostridium perfringens (CP) challenge; T2: birds with CP challenge; T3: birds with CP challenge plus HB at 1 kg/metric ton; T4: birds with CP challenge plus HB at 0.75 kg/metric ton and T5: birds with CP challenge plus HB at 0.5 kg/metric ton. At day 13, all chickens were vaccinated with 10x coccidia oocysts. At day 14, all chickens were treated with a 10x dose of infectious bursal disease vaccine. From day 15 to day 19, chickens in challenge groups (T2, T3, T4, and T5) were orally gavaged with 108 cfu of C. perfringens twice a day. At day 21, all chickens were euthanized, and NE intestinal lesion scores were recorded. Chickens in T3 and T4 exposed to a NE challenge model showed significantly reduced lesion scores compared with the rest of the groups. Furthermore, in vitro minimal inhibitory concentration (MIC) assays revealed that HB had the lowest MIC at both 24 h (390 μg/mL) and 48 h (625 μg/mL) against C. perfringens. Herbanoplex CP showed similar response to BMD in a nonspecific challenge model in promoting growth performance (Exp 1). Moreover, HB reduced the severity of intestinal lesion score using a NE challenge model (Exp 2), suggesting that the antibacterial properties against C. perfringens by HB can promote growth during microbial challenges.http://www.sciencedirect.com/science/article/pii/S1056617121000246Clostridium perfringensHumulus lupulusnecrotic enteritisperformancephytobiotic |
| spellingShingle | B. Vecchi E. Gumina F. Matte A. Bata S. Bata V. Molnar-Nagy J.W. Hall X. Hernandez-Velasco S. Layton Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model Journal of Applied Poultry Research Clostridium perfringens Humulus lupulus necrotic enteritis performance phytobiotic |
| title | Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| title_full | Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| title_fullStr | Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| title_full_unstemmed | Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| title_short | Effect of Herbanoplex CP on broiler chicken's performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| title_sort | effect of herbanoplex cp on broiler chicken s performance following a nondefined challenge or intestinal lesion score using a necrotic enteritis challenge model |
| topic | Clostridium perfringens Humulus lupulus necrotic enteritis performance phytobiotic |
| url | http://www.sciencedirect.com/science/article/pii/S1056617121000246 |
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