Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012.
BACKGROUND:The 13-valent pneumococcal vaccine (PCV13) was introduced in Cambodia in January 2015. There are limited data concerning the common serotypes causing invasive pneumococcal disease (IPD). Knowledge of the circulating pneumococcal serotypes is important to monitor epidemiological changes be...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4957771?pdf=render |
| _version_ | 1818138107537522688 |
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| author | Catrin E Moore Adam Giess Sona Soeng Poda Sar Varun Kumar Pheakdey Nhoung Rachel Bousfield Paul Turner Nicole Stoesser Nicholas P J Day Christopher M Parry |
| author_facet | Catrin E Moore Adam Giess Sona Soeng Poda Sar Varun Kumar Pheakdey Nhoung Rachel Bousfield Paul Turner Nicole Stoesser Nicholas P J Day Christopher M Parry |
| author_sort | Catrin E Moore |
| collection | DOAJ |
| description | BACKGROUND:The 13-valent pneumococcal vaccine (PCV13) was introduced in Cambodia in January 2015. There are limited data concerning the common serotypes causing invasive pneumococcal disease (IPD). Knowledge of the circulating pneumococcal serotypes is important to monitor epidemiological changes before and after vaccine implementation. METHODS:All episodes of IPD defined by the isolation of Streptococcus pneumoniae from blood, cerebrospinal fluid or other sterile site in Cambodian children admitted to the Angkor Hospital for Children in Siem Reap, Northwestern Cambodia, between 1st January 2007 and 1st July 2012 were retrospectively studied. Streptococcus pneumoniae isolates that could be retrieved underwent phenotypic typing and whole genome sequencing. RESULTS:There were 90 Cambodian children hospitalized with IPD with a median (IQR) age of 2.3 years (0.9-6.2). The case fatality was 15.6% (95% CI 8-23). Of 50 Streptococcus pneumoniae isolates available for further testing, 46% were penicillin non-susceptible and 8% were ceftriaxone non-susceptible, 78% were cotrimoxazole resistant, 30% were erythromycin resistant and 30% chloramphenicol resistant. There were no significant changes in resistance levels over the five-year period. The most common serotypes were 1 (11/50; 22%), 23F (8/50; 16%), 14 (6/50; 12%), 5 (5/50; 10%) and 19A (3/50; 6%). Coverage by PCV7, PCV10 and PCV13 was 44%, 76% and 92% respectively. We identified novel multilocus sequence types and resistotypes using whole genome sequencing. CONCLUSIONS:This study suggests IPD is an important disease in Cambodian children and can have a significant mortality. PCV13 coverage of the serotypes determined in studied strains was high and consistent with another recent study. The phenotypic resistance patterns observed were similar to other regional studies. The use of whole genome sequencing in the present study provides additional typing and resistance information together with the description of novel sequence types and resistotypes. |
| first_indexed | 2024-12-11T10:06:56Z |
| format | Article |
| id | doaj.art-813bc2beeec744bbbfc6aa117a3aa4a4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T10:06:56Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-813bc2beeec744bbbfc6aa117a3aa4a42022-12-22T01:11:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015935810.1371/journal.pone.0159358Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012.Catrin E MooreAdam GiessSona SoengPoda SarVarun KumarPheakdey NhoungRachel BousfieldPaul TurnerNicole StoesserNicholas P J DayChristopher M ParryBACKGROUND:The 13-valent pneumococcal vaccine (PCV13) was introduced in Cambodia in January 2015. There are limited data concerning the common serotypes causing invasive pneumococcal disease (IPD). Knowledge of the circulating pneumococcal serotypes is important to monitor epidemiological changes before and after vaccine implementation. METHODS:All episodes of IPD defined by the isolation of Streptococcus pneumoniae from blood, cerebrospinal fluid or other sterile site in Cambodian children admitted to the Angkor Hospital for Children in Siem Reap, Northwestern Cambodia, between 1st January 2007 and 1st July 2012 were retrospectively studied. Streptococcus pneumoniae isolates that could be retrieved underwent phenotypic typing and whole genome sequencing. RESULTS:There were 90 Cambodian children hospitalized with IPD with a median (IQR) age of 2.3 years (0.9-6.2). The case fatality was 15.6% (95% CI 8-23). Of 50 Streptococcus pneumoniae isolates available for further testing, 46% were penicillin non-susceptible and 8% were ceftriaxone non-susceptible, 78% were cotrimoxazole resistant, 30% were erythromycin resistant and 30% chloramphenicol resistant. There were no significant changes in resistance levels over the five-year period. The most common serotypes were 1 (11/50; 22%), 23F (8/50; 16%), 14 (6/50; 12%), 5 (5/50; 10%) and 19A (3/50; 6%). Coverage by PCV7, PCV10 and PCV13 was 44%, 76% and 92% respectively. We identified novel multilocus sequence types and resistotypes using whole genome sequencing. CONCLUSIONS:This study suggests IPD is an important disease in Cambodian children and can have a significant mortality. PCV13 coverage of the serotypes determined in studied strains was high and consistent with another recent study. The phenotypic resistance patterns observed were similar to other regional studies. The use of whole genome sequencing in the present study provides additional typing and resistance information together with the description of novel sequence types and resistotypes.http://europepmc.org/articles/PMC4957771?pdf=render |
| spellingShingle | Catrin E Moore Adam Giess Sona Soeng Poda Sar Varun Kumar Pheakdey Nhoung Rachel Bousfield Paul Turner Nicole Stoesser Nicholas P J Day Christopher M Parry Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. PLoS ONE |
| title | Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. |
| title_full | Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. |
| title_fullStr | Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. |
| title_full_unstemmed | Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. |
| title_short | Characterisation of Invasive Streptococcus pneumoniae Isolated from Cambodian Children between 2007 - 2012. |
| title_sort | characterisation of invasive streptococcus pneumoniae isolated from cambodian children between 2007 2012 |
| url | http://europepmc.org/articles/PMC4957771?pdf=render |
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