In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria
ABSTRACT Objective Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of ha...
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| Format: | Article |
| Language: | English |
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University of São Paulo
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| Series: | Journal of Applied Oral Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600582&lng=en&tlng=en |
| _version_ | 1819088002385707008 |
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| author | Talita Signoreti GRAZIANO Caroline Morini CALIL Adilson SARTORATTO Gilson César Nobre FRANCO Francisco Carlos GROPPO Karina COGO-MÜLLER |
| author_facet | Talita Signoreti GRAZIANO Caroline Morini CALIL Adilson SARTORATTO Gilson César Nobre FRANCO Francisco Carlos GROPPO Karina COGO-MÜLLER |
| author_sort | Talita Signoreti GRAZIANO |
| collection | DOAJ |
| description | ABSTRACT Objective Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. Material and Methods The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. Results The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H2S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H2S and methyl mercaptan (CH3SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H2S and CH3SH decreased (p<0.05, ANOVA-Dunnet). Conclusion M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine. |
| first_indexed | 2024-12-21T21:45:07Z |
| format | Article |
| id | doaj.art-813dad8fbcdd48ee878c4651283c8836 |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-21T21:45:07Z |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-813dad8fbcdd48ee878c4651283c88362022-12-21T18:49:16ZengUniversity of São PauloJournal of Applied Oral Science1678-776524658258910.1590/1678-775720160044S1678-77572016000600582In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteriaTalita Signoreti GRAZIANOCaroline Morini CALILAdilson SARTORATTOGilson César Nobre FRANCOFrancisco Carlos GROPPOKarina COGO-MÜLLERABSTRACT Objective Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. Material and Methods The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. Results The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H2S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H2S and methyl mercaptan (CH3SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H2S and CH3SH decreased (p<0.05, ANOVA-Dunnet). Conclusion M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600582&lng=en&tlng=enProducts with antimicrobial actionHalitosis. Natural productsPorphyromonas gingivalisPorphyromonas endodontalis |
| spellingShingle | Talita Signoreti GRAZIANO Caroline Morini CALIL Adilson SARTORATTO Gilson César Nobre FRANCO Francisco Carlos GROPPO Karina COGO-MÜLLER In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria Journal of Applied Oral Science Products with antimicrobial action Halitosis. Natural products Porphyromonas gingivalis Porphyromonas endodontalis |
| title | In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| title_full | In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| title_fullStr | In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| title_full_unstemmed | In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| title_short | In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| title_sort | in vitro effects of melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
| topic | Products with antimicrobial action Halitosis. Natural products Porphyromonas gingivalis Porphyromonas endodontalis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572016000600582&lng=en&tlng=en |
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