Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota
AbstractContext Slow transit constipation (STC), the most common type of constipation, seriously affects the life of patients. Zhizhu decoction (ZZD), a traditional Chinese medicine compound, has is effective against functional constipation, but the mechanism is still unclear.Objective This research...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2157020 |
| _version_ | 1827302089335767040 |
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| author | Yong Wen Yu Zhan Shiyu Tang Fang Liu Rong Wu Pengfei Kong Qian Li Xuegui Tang |
| author_facet | Yong Wen Yu Zhan Shiyu Tang Fang Liu Rong Wu Pengfei Kong Qian Li Xuegui Tang |
| author_sort | Yong Wen |
| collection | DOAJ |
| description | AbstractContext Slow transit constipation (STC), the most common type of constipation, seriously affects the life of patients. Zhizhu decoction (ZZD), a traditional Chinese medicine compound, has is effective against functional constipation, but the mechanism is still unclear.Objective This research explores the mechanism of ZZD on STC from the perspective of metabolomics and gut microbiota.Materials and methods Fifty-four C57BL/6 mice were randomly divided into six groups (n = 9): control (control); STC (model); positive control (positive); low-dose (5 g/kg; L-ZZD), medium-dose (10 g/kg; M-ZZD), and high-dose (20 g/kg; H-ZZD) ZZD treatment. Following treatment of mice with ZZD for two weeks, the changes in intestinal motility, colon histology, intestinal neurotransmitters, and aryl hydrocarbon receptor (AHR) pathway determined the effects of ZZD on the pathophysiology of STC. LC-MS targeting serum metabolomics was used to analyze the regulation of ZZD on neurotransmitters, and 16S rRNA high-throughput sequencing was used to detect the regulation of the gut microbiome.Results ZZD had the highest content of naringin (6348.1 mg/L), and could significantly increase the 24 h defecations (1.10- to 1.42-fold), fecal moisture (1.14-fold) and intestinal transport rate (1.28-fold) of STC mice, increased the thickness of the mucosal and muscular tissue (1.18- to 2.16-fold) and regulated the neurotransmitters in the colon of STC mice. Moreover, ZZD significantly activated the AHR signaling pathway, and also affected the composition of gut microbiota in STC mice.Discussion and conclusions The beneficial effect and the possible mechanism of ZZD on STC could provide a theoretical basis for the broader clinical application of ZZD. |
| first_indexed | 2024-03-09T00:09:18Z |
| format | Article |
| id | doaj.art-81444633c4104bcb970d4f8452d898e2 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:28Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-81444633c4104bcb970d4f8452d898e22024-03-29T11:10:25ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-0161111112410.1080/13880209.2022.2157020Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiotaYong Wen0Yu Zhan1Shiyu Tang2Fang Liu3Rong Wu4Pengfei Kong5Qian Li6Xuegui Tang7Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaAffiliated Hospital of Integrated Chinese Medicine and Western Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Integrated Traditional and Western Medicine Anorectal, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Integrated Traditional and Western Medicine Anorectal, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaHospital of Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Integrated Traditional and Western Medicine Anorectal, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Integrated Traditional and Western Medicine Anorectal, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaDepartment of Integrated Traditional and Western Medicine Anorectal, Affiliated Hospital of North Sichuan Medical College, Nanchong, ChinaAbstractContext Slow transit constipation (STC), the most common type of constipation, seriously affects the life of patients. Zhizhu decoction (ZZD), a traditional Chinese medicine compound, has is effective against functional constipation, but the mechanism is still unclear.Objective This research explores the mechanism of ZZD on STC from the perspective of metabolomics and gut microbiota.Materials and methods Fifty-four C57BL/6 mice were randomly divided into six groups (n = 9): control (control); STC (model); positive control (positive); low-dose (5 g/kg; L-ZZD), medium-dose (10 g/kg; M-ZZD), and high-dose (20 g/kg; H-ZZD) ZZD treatment. Following treatment of mice with ZZD for two weeks, the changes in intestinal motility, colon histology, intestinal neurotransmitters, and aryl hydrocarbon receptor (AHR) pathway determined the effects of ZZD on the pathophysiology of STC. LC-MS targeting serum metabolomics was used to analyze the regulation of ZZD on neurotransmitters, and 16S rRNA high-throughput sequencing was used to detect the regulation of the gut microbiome.Results ZZD had the highest content of naringin (6348.1 mg/L), and could significantly increase the 24 h defecations (1.10- to 1.42-fold), fecal moisture (1.14-fold) and intestinal transport rate (1.28-fold) of STC mice, increased the thickness of the mucosal and muscular tissue (1.18- to 2.16-fold) and regulated the neurotransmitters in the colon of STC mice. Moreover, ZZD significantly activated the AHR signaling pathway, and also affected the composition of gut microbiota in STC mice.Discussion and conclusions The beneficial effect and the possible mechanism of ZZD on STC could provide a theoretical basis for the broader clinical application of ZZD.https://www.tandfonline.com/doi/10.1080/13880209.2022.2157020Intestinal neurotransmittersintestinal motilitymetabolomics16S rRNA sequencingfunctional constipation |
| spellingShingle | Yong Wen Yu Zhan Shiyu Tang Fang Liu Rong Wu Pengfei Kong Qian Li Xuegui Tang Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota Pharmaceutical Biology Intestinal neurotransmitters intestinal motility metabolomics 16S rRNA sequencing functional constipation |
| title | Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| title_full | Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| title_fullStr | Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| title_full_unstemmed | Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| title_short | Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| title_sort | zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota |
| topic | Intestinal neurotransmitters intestinal motility metabolomics 16S rRNA sequencing functional constipation |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2157020 |
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