Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method
Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two regions. Using this method, we successfully l...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-09-01
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| Series: | Frontiers in Neural Circuits |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncir.2014.00110/full |
| _version_ | 1818510383074246656 |
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| author | Akiya eWatakabe Akiya eWatakabe Masafumi eTakaji Shigeki eKato Kazuto eKobayashi Hiroaki eMizukami Keiya eOzawa Sonoko eOhsawa Ryosuke eMatsui Dai eWatanabe Tetsuo eYamamori Tetsuo eYamamori |
| author_facet | Akiya eWatakabe Akiya eWatakabe Masafumi eTakaji Shigeki eKato Kazuto eKobayashi Hiroaki eMizukami Keiya eOzawa Sonoko eOhsawa Ryosuke eMatsui Dai eWatanabe Tetsuo eYamamori Tetsuo eYamamori |
| author_sort | Akiya eWatakabe |
| collection | DOAJ |
| description | Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two regions. Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral S1 for retrograde infection. The strength of this method is that we can observe the morphology of specific projection neuron subtypes en masse. We found that the group of CT cells extends their dendrites and intrinsic axons extensively below but not within the thalamorecipient layer in both S1BF and M1, suggesting that the primary target of this cell type is not layer 4. We also found that both ipsi- and contralateral targeting CC cells in M1 commonly exhibit widespread collateral extensions to contralateral M1 (layers 1-6), bilateral S1 and S2 (layers 1, 5 and 6), perirhinal cortex (layers 1, 2/3, 5 and 6), striatum and claustrum. These findings not only strengthened the previous findings of single cell tracings but also extended them by enabling cross-area comparison of CT cells or comparison of CC cells of two different labeling. |
| first_indexed | 2024-12-10T22:58:28Z |
| format | Article |
| id | doaj.art-81457d0b90f2475aa2e7b150f01bfc4d |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-10T22:58:28Z |
| publishDate | 2014-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-81457d0b90f2475aa2e7b150f01bfc4d2022-12-22T01:30:12ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102014-09-01810.3389/fncir.2014.00110106167Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection methodAkiya eWatakabe0Akiya eWatakabe1Masafumi eTakaji2Shigeki eKato3Kazuto eKobayashi4Hiroaki eMizukami5Keiya eOzawa6Sonoko eOhsawa7Ryosuke eMatsui8Dai eWatanabe9Tetsuo eYamamori10Tetsuo eYamamori11National Institute for Basic BiologySokendaiNational Institute for Basic BiologyFukushima Medical University School of MedicineFukushima Medical University School of MedicineJichi Medical UniversityJichi Medical UniversityNational Institute for Basic BiologyKyoto UniversityKyoto UniversityNational Institute for Basic BiologySokendaiHere we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two regions. Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral S1 for retrograde infection. The strength of this method is that we can observe the morphology of specific projection neuron subtypes en masse. We found that the group of CT cells extends their dendrites and intrinsic axons extensively below but not within the thalamorecipient layer in both S1BF and M1, suggesting that the primary target of this cell type is not layer 4. We also found that both ipsi- and contralateral targeting CC cells in M1 commonly exhibit widespread collateral extensions to contralateral M1 (layers 1-6), bilateral S1 and S2 (layers 1, 5 and 6), perirhinal cortex (layers 1, 2/3, 5 and 6), striatum and claustrum. These findings not only strengthened the previous findings of single cell tracings but also extended them by enabling cross-area comparison of CT cells or comparison of CC cells of two different labeling.http://journal.frontiersin.org/Journal/10.3389/fncir.2014.00110/fullcorticothalamicaavpyramidaltracerretrograde viral vectoraxon collateral |
| spellingShingle | Akiya eWatakabe Akiya eWatakabe Masafumi eTakaji Shigeki eKato Kazuto eKobayashi Hiroaki eMizukami Keiya eOzawa Sonoko eOhsawa Ryosuke eMatsui Dai eWatanabe Tetsuo eYamamori Tetsuo eYamamori Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method Frontiers in Neural Circuits corticothalamic aav pyramidal tracer retrograde viral vector axon collateral |
| title | Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method |
| title_full | Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method |
| title_fullStr | Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method |
| title_full_unstemmed | Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method |
| title_short | Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method |
| title_sort | simultaneous visualization of extrinsic and intrinsic axon collaterals in golgi like detail for mouse corticothalamic and corticocortical cells a double viral infection method |
| topic | corticothalamic aav pyramidal tracer retrograde viral vector axon collateral |
| url | http://journal.frontiersin.org/Journal/10.3389/fncir.2014.00110/full |
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