Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method

Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method

Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two regions. Using this method, we successfully l...

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Bibliographic Details
Main Authors: Akiya eWatakabe, Masafumi eTakaji, Shigeki eKato, Kazuto eKobayashi, Hiroaki eMizukami, Keiya eOzawa, Sonoko eOhsawa, Ryosuke eMatsui, Dai eWatanabe, Tetsuo eYamamori
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-09-01
Series:Frontiers in Neural Circuits
Subjects:
corticothalamic
aav
pyramidal
tracer
retrograde viral vector
axon collateral
Online Access:http://journal.frontiersin.org/Journal/10.3389/fncir.2014.00110/full

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