Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum
cognition, learning and memory functions. This study presents a permanent model of a toxin produced cerebellar lesion characterized according to contemporary motor and cognitive abnormalities. Materials and Methods: In this experimental study, slow administration of quinolinic acid (QA, 5 μl of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2014-04-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_657823232344432344232423444254683553644411-Mohammad%20Amin%20Edalatmanesh-phone.pdf |
| _version_ | 1818033750709108736 |
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| author | Mohammad Amin Edalatmanesh Haniyeh Nikfarjam Marzieh Moghadas Aliakbar Haddad-Mashadrizeh Reza Robati Mohammad Reza Hashemzadeh |
| author_facet | Mohammad Amin Edalatmanesh Haniyeh Nikfarjam Marzieh Moghadas Aliakbar Haddad-Mashadrizeh Reza Robati Mohammad Reza Hashemzadeh |
| author_sort | Mohammad Amin Edalatmanesh |
| collection | DOAJ |
| description | cognition, learning and memory functions. This study presents a permanent model
of a toxin produced cerebellar lesion characterized according to contemporary motor
and cognitive abnormalities.
Materials and Methods: In this experimental study, slow administration of quinolinic acid
(QA, 5 μl of 200 μmol, 1 μl/minute) in the right cerebellar hemisphere (lobule VI) caused noticeable
motor and cognitive disturbances along with cellular degeneration in all treated animals.
We assessed behavioral and histopathological studies over ten weeks after QA treatment. The
data were analyzed with ANOVA and the student’s t test.
Results: The QA treated group showed marked motor learning deficits on the rotating rod
test (p≤0.0001), locomotor asymmetry on the cylinder test (p≤0.0001), dysmetria on the
beam balance test (p≤0.0001), abnormalities in neuromuscular strength on the hang wire test
(p≤0.0001), spatial memory deficits in the Morris water maze (MWM, p≤0.001) and fear conditioned
memory on the passive avoidance test (p≤0.01) over a ten-week period compared with
the control animals. Histopathological analysis showed loss of Purkinje cells (p≤0.001) and
granular cell density (p≤0.0001) in the lesioned hemisphere of the cerebellum.
Conclusion: Results of the present study show that QA can remove numerous cells which
respond to this toxin in hemispheric lobule VI and thus provide a potential model for functional
and cell-based studies. |
| first_indexed | 2024-12-10T06:28:14Z |
| format | Article |
| id | doaj.art-814a86b8b2314526a23550d75f0db977 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T06:28:14Z |
| publishDate | 2014-04-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-814a86b8b2314526a23550d75f0db9772022-12-22T01:59:09ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142014-04-01163325334Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The CerebellumMohammad Amin Edalatmanesh0Haniyeh Nikfarjam1Marzieh Moghadas2Aliakbar Haddad-Mashadrizeh3Reza Robati4Mohammad Reza Hashemzadeh5Department of Physiology, Science and Research Branch, Islamic Azad University, Fars, IranDepartment of Physiology, Science and Research Branch, Islamic Azad University, Fars, IranDepartment of Physiology, Science and Research Branch, Islamic Azad University, Fars, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Physiology, Science and Research Branch, Islamic Azad University, Fars, IranDepartment of Stem cell and Regenerative Biology, Eram Biotechnology Research Center, Technical and Vocational Training Organization, Mashhad, Irancognition, learning and memory functions. This study presents a permanent model of a toxin produced cerebellar lesion characterized according to contemporary motor and cognitive abnormalities. Materials and Methods: In this experimental study, slow administration of quinolinic acid (QA, 5 μl of 200 μmol, 1 μl/minute) in the right cerebellar hemisphere (lobule VI) caused noticeable motor and cognitive disturbances along with cellular degeneration in all treated animals. We assessed behavioral and histopathological studies over ten weeks after QA treatment. The data were analyzed with ANOVA and the student’s t test. Results: The QA treated group showed marked motor learning deficits on the rotating rod test (p≤0.0001), locomotor asymmetry on the cylinder test (p≤0.0001), dysmetria on the beam balance test (p≤0.0001), abnormalities in neuromuscular strength on the hang wire test (p≤0.0001), spatial memory deficits in the Morris water maze (MWM, p≤0.001) and fear conditioned memory on the passive avoidance test (p≤0.01) over a ten-week period compared with the control animals. Histopathological analysis showed loss of Purkinje cells (p≤0.001) and granular cell density (p≤0.0001) in the lesioned hemisphere of the cerebellum. Conclusion: Results of the present study show that QA can remove numerous cells which respond to this toxin in hemispheric lobule VI and thus provide a potential model for functional and cell-based studies.http://celljournal.org/library/upload/article/af_657823232344432344232423444254683553644411-Mohammad%20Amin%20Edalatmanesh-phone.pdfQuinolinic AcidCerebellumCognitionPurkinje CellGranular Cell |
| spellingShingle | Mohammad Amin Edalatmanesh Haniyeh Nikfarjam Marzieh Moghadas Aliakbar Haddad-Mashadrizeh Reza Robati Mohammad Reza Hashemzadeh Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum Cell Journal Quinolinic Acid Cerebellum Cognition Purkinje Cell Granular Cell |
| title | Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum |
| title_full | Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum |
| title_fullStr | Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum |
| title_full_unstemmed | Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum |
| title_short | Histopathological and Behavioral Assessment of Toxin-Produced Cerebellar Lesion: A Potent Model for Cell Transplantation Studies in The Cerebellum |
| title_sort | histopathological and behavioral assessment of toxin produced cerebellar lesion a potent model for cell transplantation studies in the cerebellum |
| topic | Quinolinic Acid Cerebellum Cognition Purkinje Cell Granular Cell |
| url | http://celljournal.org/library/upload/article/af_657823232344432344232423444254683553644411-Mohammad%20Amin%20Edalatmanesh-phone.pdf |
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